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Recombinant glucose-fructose oxidoreductase and fungal expression vector as well as fungal insecticide thereof

A technology of glucose and reductase, applied in the field of molecular biology, to achieve the effect of accelerating reproduction, avoiding removal, and improving insecticidal effect

Active Publication Date: 2015-02-18
SOUTHWEST UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

However, whether this gene can be introduced into insecticidal fungi to produce gluconolactone during fungal infection, inhibit the immune response of the host, and improve the insecticidal effect of fungi has not yet been reported.

Method used

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  • Recombinant glucose-fructose oxidoreductase and fungal expression vector as well as fungal insecticide thereof
  • Recombinant glucose-fructose oxidoreductase and fungal expression vector as well as fungal insecticide thereof
  • Recombinant glucose-fructose oxidoreductase and fungal expression vector as well as fungal insecticide thereof

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Embodiment Construction

[0041] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0042] A recombinant glucose-fructose oxidoreductase, containing a fusion protein composed of secreted signal peptide derived from Beauveria bassiana chitinase and glucose-fructose redox derived from Zymomonas mobilis The mature protein region of the enzyme gene is fused.

[0043] The nucleotide sequence of the recombinant glucose-fructose oxidoreductase of the present invention was constructed by using molecular biology technology, which contains the coding sequence of Beauveria bassiana ( Beauveria bassiana ) The signal peptide sequence of chitinase is identical to that encoded by Zymomonas mobilis ( Zymonomas mobilis ) The mature protein region sequence of glucose-fructose oxidoreductase has the nucleotide sequence shown in SEQ ID NO. 1 and the amino acid sequence shown in SEQ ID NO. 2.

[0044] The present invention also provides a reco...

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Abstract

The invention provides a recombinant glucose-fructose oxidoreductase and a fungal expression vector as well as a fungal insecticide thereof. A recombinant nucleotide sequence, which comprises a signal peptide sequence for coding the chitinase of beauveria bassiana and a mature protein region sequence for coding the glucose-fructose oxidoreductase of zymonomas mobilis, is constructed by use of the molecular biological technique. According to the recombinant glucose-fructose oxidoreductase, the PgpdA-BbSP-GFOR segment is cloned into a pUC-Bar vector to obtain the fungal expression vector; the fungal expression vector is imported into the wild-type beauveria bassiana Bb0062 strain to obtain the beauveria bassiana transformant of BbSP-GFOR. The recombinant glucose-fructose oxidoreductase effectively blocks the activity of the GNBPs in a host; the time that the fungi knock down insect pests is shortened by 48 hours and the insecticidal efficacy is remarkably improved.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a recombinant glucose-fructose oxidoreductase and its fungal expression vector and fungal pesticide, in particular to a glucose-fructose oxidoreductase GFOR containing fungal secreted signal peptide and bacteria Sequence recombinant protein and its fungal expression vector and fungal insecticide. [0002] Background technique [0003] my country is a large agricultural production country, and the grain output has been harvested year after year, and the use of pesticides has contributed a lot. However, data show that the average amount of pesticides used in my country is 2.5 times the world average. Excessive use of pesticides has polluted the environment and threatened the quality and safety of agricultural products and food safety. Therefore, developing high-efficiency, environmentally safe, and specific biopesticides and reducing the use of conventional chemical pesticides will...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/62C12N15/80A01N63/04A01P7/04
CPCA01N63/30C12N9/0006C12N15/80C12Y101/99028
Inventor 范艳华内马特·肯哈尼裴炎张永军金丹罗志兵
Owner SOUTHWEST UNIVERSITY
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