140 results about "Beauveria amorpha" patented technology

The invention belongs to the technical field of environment protection, and especially relates to a composite microbial preparation for garbage deodorizing and sterilization and a preparation method thereof. The microbial preparation comprises beauveria bassiana, saccharomyces cerevisiae, lactobacillus plantar, rhodopseudomonas palustris, and bacillus thruingiensis. The comprehensive composite inoculant provided by the invention can remove the garbage odor and kill insects such as mosquito, fly, cockroach, and the like; and can be used in locations such as rubbish transfer station, digestion tank, public lavatory, landfill, etc. to remove odors and kill insects. The stinky odors given off by the garbage can be rapidly degraded under the cooperation of five microorganisms, furthermore, the bacterium proliferation is inhibited, the insects in the garbage piles such as mosquito, fly, cockroach, and the like can be killed, and the microecological environment of garbage piles can be improved, so the composite inoculant has a very important economic and environment-protection meaning.

Relating to technical field of microorganisms, the invention discloses a beauveria bassiana XNBb-04 strain and a culture method thereof. The beauveria bassiana XNBb-04 strain is preserved in China Center for Type Culture Collection on March 6, 2014, and the preservation number is CCTCC M 2014075. The beauveria bassiana XNBb-04 strain provided by the invention belongs to Deuteromycota, Moniliales, Moniliaceae, Beauveria. The beauveria bassiana XNBb-04 strain is a native entomopathogenic fungus of China, can be further used for preparation of living biopesticides, has good pathogenic effect on nodulated alfalfa weevils, can effectively kill nodulated alfalfa weevils, has brand new action mechanism different from existing chemical insecticides, and has no environmental pollution or residue. The beauveria bassiana XNBb-04 strain provided by the invention can be applied to various ecological environments in terms of pest control, and is conducive to environmental protection.

The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

The invention relates to a preparation method and application of q beauveria-spinosad suspending agent. Beauveryia bassiana and spinosad are the active ingredients of the suspending agent which is used for preventing and treating agriculture and forestry injurious insects, such as leptinotarsa decemlineata and acridid and the like. The invention is characterized in that (1) the suspending agent comprises active ingredients, namely the beauveryia bassiana and the spinosad; and (2) the mass ratio of the beauveryia bassiana and the spinosad is (30:1)-(30:2), and the mass percent of the effective components in the preparation is 3.1-10%. The preparation method has the advantages that the product can be dissolved with oil or water; the effective duration of the beauveria vuillemin and the relative quick action of spinosad have complementary advantages, and injurious insects of various crops are treated; and the suspending agent prepared by the method has the advantages of good synergism and permeability, strong adhesivity and evaporation resistance and the like.

An agricultural chemical for preventing and killing pine moth is prepared from Beauveria bassiana (CGMCC No.1573) through slant culture, culturing in jar, culturing in bag, and mixing with nutritive wet material. It has high effect (70-80%) and no pollution.

The invention discloses a recombinant serine protease inhibitor, a fungus expression vector and a fungus insecticide of recombinant protein. The invention relates to the recombinant serine protease inhibitor; a molecular biology technology is used to construct a recombinant nucleotide sequence; and the recombinant serine protease inhibitor comprises a signal peptide sequence of encoded coccidio beauveriabassiana chitinase and a serine protease inhibitor gene of encoded drosophila, and has a nucleotide sequence shown as SEQ ID NO. 1 and an amino acid sequence shown as SEQ ID NO. 2. The obtained recombinant serine protease inhibitor can inhibit immunoreaction of insects, increase multiplication speed of fungi in haemocoeles, and therefore improve characteristics of insecticide fungus toxicity.

Provided are compositions derived from Beauveria bassiana, Brevibacillus brevis, Streptomyces spororaveus, Paenibacillus elgii, or Streptomyces sp. fermentation, having algicidal properties, and method of use thereof.

The invention relates to the technical field of microbial colonization and concretely relates to a method for colonizing Beauveria bassiana in a corn seedling stage and a use thereof. The method comprises disinfecting surfaces of corn seeds, and soaking the seeds in a Beauveria bassiana spore suspension, wherein the Beauveria bassiana strain is preserved in the China general microbiological culture collection center on January 23, 2013 and has an accession number of CGMCC7192. The method is simple and easy and ensures the optimal seed soaking time and colonization preference of Beauveria bassiana in a corn seedling stage. Through colonization of entomogenous fungi in a plant, plant resistance is improved so that pathogen-caused damage to plants is reduced and plant pests are avoided through indirect regulation and control of phytophagous insects.

The invention discloses a Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta. The strain is preserved at China Center for Type Culture Collection on February 16, 2017 and the preservation number is CCTCC NO: M2017045. The Beauveria bassiana BbL25 strain is highly pathogenic to the solenopsis invicta, and half lethal concentration (LC50) to the solenopsis invicta is 8.15x10<5> conidium / mL on the 14th day after inoculation, and the strain has a good field controlling effect on the solenopsis invicta; the controlling effect of 1x10<7> / mL condinal suspension on the solenopsis invicta reaches 82.52% on the 14th day after drug delivering, and the effect is same as chemical insecticide 0.25% fluoxane. The Beauveria bassiana BbL25 strain can be used as a living biological insecticide and has no pollution to the environment and is non-toxic to human beings and animals and is suitable for the production of organic agricultural products, and great application prospects are achieved.

The invention discloses a complex microbial inoculant for straw compost maturity and european corn borer prevention, as well as preparation and application of the complex microbial inoculant. The complex microbial inoculant comprises a mixed strain and an additive, wherein the mixed strain comprises trichoderma harzianum, bacillus licheniformis and beauveria bassiana, and is prepared by the following steps: inoculating three bacterial strain test tube slant culture media subjected to purification and rejuvenation to corresponding fluid nutrient media respectively, performing fermentation to obtain liquid strains, inoculating the three fermented fluid strains into a solid culture medium, culturing for 48-72h at the temperature being 28-30 DEG C, dying, and crushing; the additive comprises glucose, superphosphate and humic acid; the mixed strain and the additive are mixed according to a mass ratio being (2.0-8.0):1.0 so as to obtain the complex microbial inoculant. The mixed strain and the additive are mixed so as to play a relatively good synergy role; the complex microbial inoculant has the capabilities of increasing the speed of straw compost maturity, killing european corn borer larvae and pupae, reducing the incidence of european corn borer, reducing the harm, improving granhle soil structures, increasing the fertilizer utilization rate and playing a yield increasing role.

The invention relates to a beauveria bassiana strain having insect intestinal infection effect, and an application thereof in pest prevention and treatment. In particular, the invention relates to beauveria bassiana which is assigned the accession number of CGMCC No.13180, or spores, mycelia or a fermented product of the beauveria bassiana, or a composition including the strain or the spores thereof, as well as a preparation method thereof, and application of the products in pest prevention and treatment. Herein, the strain and the composition have synergistic effect in pest prevention and treatment (e.g. lepidoptera and dipteran insects) in a unique manner of intestinal infection and intestinal / integument dual-infection, so that the strain has extensive application prospect.

The beauveria bassiana SB063 and the spinetoram in an insecticide disclosed by the invention have a remarkable synergistic effect on the poisoning and killing effects of megalurothrips usitatus. Long-term infection biological research and indoor biological assay show that the combined use of beauveria bassiana and spinetoram has a good prevention and treatment effect on megalurothrips usitatus, and the insecticide has a very strong application potential in biological prevention and treatment, can reduce the use amount of chemical pesticides, has the characteristics of low toxicity and low drug resistance, does not pollute the environment, does not have residues, and can favorably delay the occurrence and development of the insecticide resistance of thrip pests.

The invention discloses a protease inhibitor BmSPI38, which comprises the 23rd and 80th amino acids of SEQIDNo. 2. The temperature and the pH of the BmSPI38 are very stable, the activity of subtilisin, proteinase K, degrading protease CDEP-1 on the wall of beauveria bassiana and semialkaline paotease can be inhibited strongly, the CDEP-1-induced excessive or harmful blackening of silkworms can be blocked, and the protease inhibitor can be applied in preparation of subtilisin inhibitors, blockers against CDEP-1-induced insecticidal blackening reaction, and can also be used for culturing silkworms with pathogenic fungi resistance and improving the resistance of the silkworms against invasion of pathogenic fungi. In addition, the operation of the BmSPI38 prokaryotic expression is simple, and the expression quantity is large.

The invention discloses a growth promoting endophytic beauveria bassiana strain and application thereof. The endophytic beauveria bassiana strain is Beauveria bassiana Bb4, is registered and preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 28, 2014, and the preservation number is CGMCC No.9097. The strain is inoculated to maize, wheat and the like, can colonize in a host body and promote the growth of the host plant, and has certain toxicity to corn borer larvae. Application of the strain to production of microbial fertilizer preparations is conducive to reducing pesticide residues and protecting the ecological environment.

The invention provides a method for improving high-seepage property, oxidative stress resistance and toxicity of beauveria bassiana by utilizing genetic engineering. The method comprises the steps of: replacing partial sequences of Bbtmp1 gene coding regions of the beauveria bassiana by utilizing bar expression element sequences, and breaking Bbtmp1 genes of the beauveria bassiana, so as to obtain a mutant strain of the beauveria bassiana with high seepage property and the improved oxidative stress resistance and toxicity. The invention further provides a fungus pesticide containing the mutant strain of the beauveria bassiana and an application of the mutant strain of the beauveria bassiana in preparation of the fungus pesticide.

The invention discloses beauveria bassiana capable of catalyzing an asymmetric reduction reaction, the bacterial strain is preserved in a China microorganism culture preservation management council common microorganism center, a preservation number is CGMCC NO.6345, and preservation time is July 12th, 2012. The bacterial strain can express carbonyl reductase high in stereoselectivity. When the bacterial strain is used for conducting whole-cell catalysis of 4-ethyl acetoacetate, enantiomer excess of S-4-chlorine-ethyl-3-hydroxybutyrate in products is 100% e.e.; and when the bacterial strain is used for conducting whole-cell catalysis of N,N-dimethyl-3-oxo-3-(2-thiophene)-1-propylamine hydrochloride, enantiomer excess of (S)-N,N-dimethyl-3-hydroxy-3-(2-thiophene)-1- propylamine hydrochloride is 100% e.e..

The invention discloses a pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as a preparation method and application thereof. The pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension disclosed by the invention comprises effective active components, wherein the active components comprise a pine moth cytoplasmic polyhedrosis virus suspension and beauveria bassiana powder; the active components account for 2-30% of the total mass of the whole preparation. The suspension is safe and non-toxic, has the characteristics ofhigh efficiency and broad spectrum, is harmless to livestock and human beings and is harmless to natural enemies.

The beauveria bassiana SB038 and the spinetoram in an insecticide disclosed by the invention have a remarkable synergistic effect on the poisoning and killing effects of megalurothrips usitatus. Long-term infection biological research and indoor biological assay show that the combined use of beauveria bassiana and spinetoram has a good prevention and treatment effect on megalurothrips usitatus, and the insecticide has a very strong application potential in biological prevention and treatment, can reduce the use amount of chemical pesticides, has the characteristics of low toxicity and low drug resistance, does not pollute the environment, does not have residues, and can favorably delay the occurrence and development of the insecticide resistance of thrip pests.

The invention relates to the field of agricultural microorganisms, and discloses a beauveria bassiana and an application thereof. The preservation number of the beauveria bassiana disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 21047. In addition, the invention also provides an application of the beauveria bassiana in preparation of a hydroxamic acid type siderophore and regulation of plant diseases. The beauveria bassiana disclosed by the invention is a dominant strain of high-yield siderophore, and can be used for synthesizing and secreting the hydroxamic acid type siderophore. The secondary metabolites of the beauveria bassiana disclosed by the invention have antibacterial activity on pseudomonas solanacearum, pectobacterium carotovorumand ralstonia solanacearum.

The invention discloses a method for producing a heavy metal absorbent through microbiological treatment on lignite and a related compound microbial agent, relating to a method for preparing the heavy metal absorbent through the microbiological treatment. The compound microbial agent is composed of fermentation liquor of beauveria bassiana, bacillus subtilis and candida tropicalis. The compound microbial agent can react with various groups in the lignite, so that the capacity of the lignite for adsorbing heavy metal ions is further promoted, and the synergy of the three microbial strains is superior to the effect of any one microbial strain and the effect of any two microbial strains.

The invention discloses a Beauveria bassiana-buprofezin compound pesticide and application thereof. The compound pesticide is composed of the following components in percentage by mass: 5-65% of main agent and 35-95% auxiliary agent. The main agent is a mixture of Beauveria bassiana and buprofezin in a mass ratio of 100:(1-20); and the auxiliary agent is composed of a stabilizer, assistants and a filler. The compound pesticide can be processed into wettable powder, suspending agents or oil solutions. The long-term infective biological research and indoor bioassay indicate that the Beauveria bassiana has high infective pesticidal effect on multiple pests, especially pests with piercing-sucking mouthparts. The compound pesticide has favorable preventing and treating effect on sweetpotato whitefly, Trialeurodes vaporariorum and other aleyrodids, and smaller green leafhoppers of tea and other leafhoppers.

The invention belongs to the technical field of microorganisms, and particularly relates to beauveria bassiana USCM6, a fungicide, application of the beauveria bassiana USCM6 and a pest control method. The invention provides Beauveria bassiana Bb0901, and the collection number of the Beauveria bassiana Bb0901 is CGMCC (China General Microbiological Culture Collection Center) No.23232. The Beauveria bassiana Bb0901 can be used for preparing a strain of Beauveria bassiana. The strain provided by the invention can effectively prevent and treat spodoptera frugiperda, and has the advantages of environmental protection and difficult generation of resistance.

The invention discloses an insecticidal composition containing beauveria bassiana and spinetoram. The insecticidal composition is characterized by being prepared from an effective component A, i.e., the beauveria bassiana, and an effective component B, i.e., the spinetoram, wherein the quantity of the effective component A, i.e., the beauveria bassiana, is 5 to 40 billion spores per gram; the content of the effective component B, i.e., the spinetoram, is 5 percent to 50 percent; the preferable quantity of the effective component A, i.e., the beauveria bassiana, is 20 to 40 billion spores per gram; the preferable content of the effective component B, i.e., the spinetoram, is 10 percent to 30 percent. The insecticidal composition disclosed by the invention has a remarkable synergetic effect;the dosage of pesticide can be reduced, the residual amount of the pesticide on crops is reduced and the environment pollution is alleviated; the insecticidal composition is safe to human and livestock and has good environmental compatibility, and insect pests do not easily have drug resistance. The insecticidal composition has a function of preventing and controlling lepidoptera pests and especially has a remarkable prevention and control effect on cabbage / plutella xylostella, corn / ostrinia nubilalis and paddy rice / cnaphalocrocis medinalis.

The invention provides a method for improving the beauveria bassiana chitinase gene disease resistance and culturing disease resistance plants adopting the method. The method is characterized in that a chitin binding structure domain of silkworm chitinase genes is fused with beauveria bassiana chitinase genes, and the disease resistance of the beauveria bassiana chitinase genes is improved. A 35S promoter is used for controlling fusion genes SwBbchit1, in addition, plant expression vectors are built and are respectively transferred into cotton and tomatoes, and the disease resistance of the transgenic cotton and tomatoes can be further improved. Compared with the disease resistance of the Bbchit1 transgenic cotton and tomatoes, the disease resistance of the SwBbchit1 cotton and tomatoes obtained by the method provided by the invention is obviously improved.

The invention discloses a strain of beauveria bassiana bh001 for preventing and treating curculio arakawai pests and application thereof. The invention provides the beauveria bassiana bh001 which is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No.17975. The biocontrol strain of the beauveria bassiana bh001 provided by the invention can be used for effectively preventing and treating curculio arakawai, has a good biocontrol potential, provides new resources for biological control on forest pests of the curculio arakawai, andalso provides help for protecting Mongolian oak seed resources.

The invention discloses a composition containing a microorganism complex. The composition containing the microorganism complex comprises, by weight, 1-2 parts of Bacillus cereus, 1-2 part sof thiamethoxam and 5-25 parts of Beauveria bassiana. The pesticide composition containing the microorganism complex can be processed to prepare a wettable powder, a water-dispersible granule, a missible oil, amicroemulsion, a suspending agent or a dispersible oil suspending agent, is used for controlling pests and diseases of rice, peanut, soybean, potato, citrus, beet, rape, pepper, cucumber and eggplant,and has the use of controlling Lepidoptera, Coleoptera, Homoptera and Diptera pests, underground pests and soil-borne bacterial diseases on vegetables. The pesticide composition containing the microorganism complex can reduce the use amount of pesticides and delay the drug resistance, has a better control effect than the single doses, and reduces the cost of farmers.

The invention relates to a recombined insect molting hormone inactivation gene Bbsp::egt and an insecticidal fungistatic agent thereof. The recombined insect molting hormone inactivation gene Bbsp::egt contains coding beauveria bassiana chitinase signal peptide sequences (Bbsp) and coding lymantria dispar nuclepolyhedrovirus (hereinafter referred to as LdMNPV) ecdysteroid uridine 5minute-diphosphonic acid-glucosyltransferase gene (egt), and has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The molecular biological technique is adopted to shift into beauveria bassiana to obtain correct genetic transformation bacterial strains. The recombined Bbsp::egt bacterial strain obtained by the invention can disturb the normal operation of a hormone system in vivo of insects, and is beneficial to reducing immune resistance of insects on insecticidal fungi beauveria bassiana and accelerating knockout of fungus on hosts, so that the insecticidal fungi virulence is improved.

The invention discloses a protease inhibitor BmSPI38, which is composed of the 23rd to 80th amino acids in SEQ ID No.2. BmSPI38 is very stable to temperature and pH, and can not only strongly inhibit subtilisin, proteinase K, and Beauveria bassiana The activity of wall degrading protease CDEP-1 and Aspergillus melissae protease, and can block the excessive and harmful melanization of silkworm induced by CDEP-1, can be used for the preparation of subtilisin protease inhibitors, and can also be used for the preparation of CDEP-1 induced The blocking agent of the insecticidal melanization reaction can also be used to cultivate silkworm strains with resistance to pathogenic fungi, and improve the ability of silkworms to resist the invasion of pathogenic fungi; in addition, the BmSPI38 prokaryotic expression method of the present invention is simple to operate, and the expression level high.

The invention discloses a strain of spodoptera frugiperda beauveria bassiana Bbsfa202007 and application thereof, the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is Beauveria bassiana, the preservation name of the strain is Beauveria bassiana, and the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is a strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 and application of the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The strain is separated from the adult body of the spodoptera frugiperda for the first time, and is relatively simple to culture, rapid in growth and large in sporulation quantity. The compound has strong pathogenicity to spodoptera frugiperda pupa, is environment-friendly and pollution-free, is not easy to generate drug resistance, and can be widely used for preventing and treating spodoptera frugiperda pupa.

The invention belongs to the technical field of microorganisms, and particularly relates to beauveria bassiana with a function of infecting young silkworms, a fungicide, application of the fungicide and a preparation method of white muscardine silkworms. The Beauveria bassiana provided by the invention comprises Beauveria bassiana UMCM2, and is characterized in that the Beauveria bassiana UMCM2 can be used for preparing the Beauveria bassiana. The strain provided by the invention has high pathogenicity to young silkworms, has the advantages of high stiffness rate and high sporulation quantity, and has wide application prospects in production and research of white muscardine silkworms.