138 results about "Beauveria amorpha" patented technology

Relating to technical field of microorganisms, the invention discloses a beauveria bassiana XNBb-04 strain and a culture method thereof. The beauveria bassiana XNBb-04 strain is preserved in China Center for Type Culture Collection on March 6, 2014, and the preservation number is CCTCC M 2014075. The beauveria bassiana XNBb-04 strain provided by the invention belongs to Deuteromycota, Moniliales, Moniliaceae, Beauveria. The beauveria bassiana XNBb-04 strain is a native entomopathogenic fungus of China, can be further used for preparation of living biopesticides, has good pathogenic effect on nodulated alfalfa weevils, can effectively kill nodulated alfalfa weevils, has brand new action mechanism different from existing chemical insecticides, and has no environmental pollution or residue. The beauveria bassiana XNBb-04 strain provided by the invention can be applied to various ecological environments in terms of pest control, and is conducive to environmental protection.

The invention relates to a preparation method and application of q beauveria-spinosad suspending agent. Beauveryia bassiana and spinosad are the active ingredients of the suspending agent which is used for preventing and treating agriculture and forestry injurious insects, such as leptinotarsa decemlineata and acridid and the like. The invention is characterized in that (1) the suspending agent comprises active ingredients, namely the beauveryia bassiana and the spinosad; and (2) the mass ratio of the beauveryia bassiana and the spinosad is (30:1)-(30:2), and the mass percent of the effective components in the preparation is 3.1-10%. The preparation method has the advantages that the product can be dissolved with oil or water; the effective duration of the beauveria vuillemin and the relative quick action of spinosad have complementary advantages, and injurious insects of various crops are treated; and the suspending agent prepared by the method has the advantages of good synergism and permeability, strong adhesivity and evaporation resistance and the like.

Provided are compositions derived from Beauveria bassiana, Brevibacillus brevis, Streptomyces spororaveus, Paenibacillus elgii, or Streptomyces sp. fermentation, having algicidal properties, and method of use thereof.

The invention relates to the technical field of microbial colonization and concretely relates to a method for colonizing Beauveria bassiana in a corn seedling stage and a use thereof. The method comprises disinfecting surfaces of corn seeds, and soaking the seeds in a Beauveria bassiana spore suspension, wherein the Beauveria bassiana strain is preserved in the China general microbiological culture collection center on January 23, 2013 and has an accession number of CGMCC7192. The method is simple and easy and ensures the optimal seed soaking time and colonization preference of Beauveria bassiana in a corn seedling stage. Through colonization of entomogenous fungi in a plant, plant resistance is improved so that pathogen-caused damage to plants is reduced and plant pests are avoided through indirect regulation and control of phytophagous insects.

The invention discloses a complex microbial inoculant for straw compost maturity and european corn borer prevention, as well as preparation and application of the complex microbial inoculant. The complex microbial inoculant comprises a mixed strain and an additive, wherein the mixed strain comprises trichoderma harzianum, bacillus licheniformis and beauveria bassiana, and is prepared by the following steps: inoculating three bacterial strain test tube slant culture media subjected to purification and rejuvenation to corresponding fluid nutrient media respectively, performing fermentation to obtain liquid strains, inoculating the three fermented fluid strains into a solid culture medium, culturing for 48-72h at the temperature being 28-30 DEG C, dying, and crushing; the additive comprises glucose, superphosphate and humic acid; the mixed strain and the additive are mixed according to a mass ratio being (2.0-8.0):1.0 so as to obtain the complex microbial inoculant. The mixed strain and the additive are mixed so as to play a relatively good synergy role; the complex microbial inoculant has the capabilities of increasing the speed of straw compost maturity, killing european corn borer larvae and pupae, reducing the incidence of european corn borer, reducing the harm, improving granhle soil structures, increasing the fertilizer utilization rate and playing a yield increasing role.

The invention discloses a growth promoting endophytic beauveria bassiana strain and application thereof. The endophytic beauveria bassiana strain is Beauveria bassiana Bb4, is registered and preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 28, 2014, and the preservation number is CGMCC No.9097. The strain is inoculated to maize, wheat and the like, can colonize in a host body and promote the growth of the host plant, and has certain toxicity to corn borer larvae. Application of the strain to production of microbial fertilizer preparations is conducive to reducing pesticide residues and protecting the ecological environment.

The invention provides a method for improving high-seepage property, oxidative stress resistance and toxicity of beauveria bassiana by utilizing genetic engineering. The method comprises the steps of: replacing partial sequences of Bbtmp1 gene coding regions of the beauveria bassiana by utilizing bar expression element sequences, and breaking Bbtmp1 genes of the beauveria bassiana, so as to obtain a mutant strain of the beauveria bassiana with high seepage property and the improved oxidative stress resistance and toxicity. The invention further provides a fungus pesticide containing the mutant strain of the beauveria bassiana and an application of the mutant strain of the beauveria bassiana in preparation of the fungus pesticide.

The invention discloses beauveria bassiana capable of catalyzing an asymmetric reduction reaction, the bacterial strain is preserved in a China microorganism culture preservation management council common microorganism center, a preservation number is CGMCC NO.6345, and preservation time is July 12th, 2012. The bacterial strain can express carbonyl reductase high in stereoselectivity. When the bacterial strain is used for conducting whole-cell catalysis of 4-ethyl acetoacetate, enantiomer excess of S-4-chlorine-ethyl-3-hydroxybutyrate in products is 100% e.e.; and when the bacterial strain is used for conducting whole-cell catalysis of N,N-dimethyl-3-oxo-3-(2-thiophene)-1-propylamine hydrochloride, enantiomer excess of (S)-N,N-dimethyl-3-hydroxy-3-(2-thiophene)-1- propylamine hydrochloride is 100% e.e..

The invention relates to a recombined insect molting hormone inactivation gene Bbsp::egt and an insecticidal fungistatic agent thereof. The recombined insect molting hormone inactivation gene Bbsp::egt contains coding beauveria bassiana chitinase signal peptide sequences (Bbsp) and coding lymantria dispar nuclepolyhedrovirus (hereinafter referred to as LdMNPV) ecdysteroid uridine 5minute-diphosphonic acid-glucosyltransferase gene (egt), and has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The molecular biological technique is adopted to shift into beauveria bassiana to obtain correct genetic transformation bacterial strains. The recombined Bbsp::egt bacterial strain obtained by the invention can disturb the normal operation of a hormone system in vivo of insects, and is beneficial to reducing immune resistance of insects on insecticidal fungi beauveria bassiana and accelerating knockout of fungus on hosts, so that the insecticidal fungi virulence is improved.

The invention discloses a protease inhibitor BmSPI38, which is composed of the 23rd to 80th amino acids in SEQ ID No.2. BmSPI38 is very stable to temperature and pH, and can not only strongly inhibit subtilisin, proteinase K, and Beauveria bassiana The activity of wall degrading protease CDEP-1 and Aspergillus melissae protease, and can block the excessive and harmful melanization of silkworm induced by CDEP-1, can be used for the preparation of subtilisin protease inhibitors, and can also be used for the preparation of CDEP-1 induced The blocking agent of the insecticidal melanization reaction can also be used to cultivate silkworm strains with resistance to pathogenic fungi, and improve the ability of silkworms to resist the invasion of pathogenic fungi; in addition, the BmSPI38 prokaryotic expression method of the present invention is simple to operate, and the expression level high.

The invention discloses a strain of spodoptera frugiperda beauveria bassiana Bbsfa202007 and application thereof, the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is Beauveria bassiana, the preservation name of the strain is Beauveria bassiana, and the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 is a strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007 and application of the strain of the spodoptera frugiperda beauveria bassiana Bbsfa202007. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is May 31, 2021; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The strain is separated from the adult body of the spodoptera frugiperda for the first time, and is relatively simple to culture, rapid in growth and large in sporulation quantity. The compound has strong pathogenicity to spodoptera frugiperda pupa, is environment-friendly and pollution-free, is not easy to generate drug resistance, and can be widely used for preventing and treating spodoptera frugiperda pupa.