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Beauveria bassiana preparation used for preventing and controlling pine moth, its preparation method and use

A technology of Beauveria bassiana and pine caterpillars is applied in the fields of botanical equipment and methods, chemicals for biological control, applications, etc., and can solve the problems of inability to germinate spores, spores dry and die due to dehydration, and freeze to death at low temperature. To achieve the effect of promoting the occurrence of epidemics, the concentration of applied bacteria, and the rapid spread of bacteria

Inactive Publication Date: 2006-08-30
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the growth and reproduction of Beauveria bassiana requires high humidity and appropriate temperature, its application is still limited to 12 provinces and regions in the south, mainly to control the masson pine caterpillar.
There are two main reasons for restricting the application of Beauveria bassiana in the north. First, there is a lack of local excellent strains adapted to the arid and low temperature environment of northern forests.
The Beauveria bassiana strains used in the south are isolated from the infected pine pine caterpillars. They generally have the genetic characteristics of adapting to the hot and humid climate in the south and adapting to parasitism on the original host pine pine caterpillars. They cannot adapt well to the north.
Second, there is a lack of suitable formulations and application techniques suitable for use in boreal forest environments
However, in the north, due to the dry and low temperature in the forest, after applying the above-mentioned formulations and methods, the spores of Beauveria bassiana will soon dry out or freeze to death due to dehydration, or the spores cannot germinate or their vitality will decrease due to lack of water absorption. Failure to successfully infect, making control failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 Preparation of nutritional wet fungus powder Beauveria bassiana preparation of the present invention

[0027] (1) Cultivation of first-level slant strains:

[0028] 1. Preparation of slant medium:

[0029] Slant medium components and their proportions: 200g of potatoes, 20g of glucose, 20g of agar, 20g of peptone, 30g of dried insect corpses, and 1000mL of water. Its preparation steps:

[0030] ①. Weigh 30g of the dry corpse of 3-5 instar Chinese pine caterpillar larvae, add 1000mL of clear water and soak for 2 hours.

[0031] ②. Peel the potatoes and dig out the buds, weigh 200g, cut into small pieces with a length and width of about 1cm, add them to the soaked dry insect corpse liquid and boil them together, until the potatoes are soft, filter them with gauze, and take the filtrate.

[0032] ③. Add water to the filtrate to 1000mL, add 20g of peptone, and heat until completely dissolved.

[0033] ④. During the boiling process, add 20g of agar, add 20g ...

Embodiment 2

[0057] The forest application test of embodiment 2 nutrient type wet fungus powder Beauveria bassiana preparation:

[0058] (1) Application of nutrient-type wet fungus powder Beauveria bassiana preparation in northern winter: In November 2003, it was applied in the 35-year-old artificial Pinus tabulaeformis forest of Xishan Mineral Bureau Forest Farm, Lingqiu County, northern Shanxi Province, and the control area was 15 hectares. Bring the prepared nutrient-type wet fungus powder to the forest where the pine caterpillar occurs and apply it. Gently dig open the litter hidden by the pine caterpillar at the base of the tree root, spread the fungal powder evenly, and then cover the litter. The dosage is 250g / plant.

[0059] Effect inspection: In late April, before the pine caterpillars sting on the tree, the control effect is checked by sampling method. Count the percentages of healthy living insects and infected dead, and then calculate the corrected death based on the control,...

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PUM

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Abstract

An agricultural chemical for preventing and killing pine moth is prepared from Beauveria bassiana (CGMCC No.1573) through slant culture, culturing in jar, culturing in bag, and mixing with nutritive wet material. It has high effect (70-80%) and no pollution.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a Beauveria bassiana preparation for controlling pine caterpillars, in particular to a Beauveria bassiana preparation suitable for preventing and controlling pine caterpillars in northern arid areas, and its preparation method and application. Background technique [0002] Pine forest is the main type of forest in our country, and it is the main content of natural forest protection projects. It plays an important role in the ecological security of our country, especially in the ecologically fragile areas in the north, where pine forest plays a huge role as a barrier. However, pine caterpillars have always been the main pests of pine forests. There are 27 species (including 4 subspecies) of pine caterpillars in China, among which the masson pine caterpillar is the most widely distributed and the most harmful in the south. In the north, Chinese pine caterpillars, red pine caterpillars, H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P5/00
Inventor 谢映平原贵生薛皎亮王国昌牛宇范丽华
Owner SHANXI UNIV
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