Protease inhibitor BmSPI38 and preparation method and application thereof
A technology of protease inhibitors and proteases, which is applied in the direction of protease inhibitors, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems such as unclear specific mechanisms, and improve the ability to resist the invasion of pathogenic fungi, Simple operation and high expressive effect
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[0042] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. Experimental methods not indicating specific conditions in the examples are usually in accordance with conventional conditions, such as described in Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang et al., Science Press, 2002) conditions, or as recommended by the manufacturer.
[0043] The silkworm species used in the examples is Dazao, provided by the Silkworm Gene Resource Bank of Southwest University of China. The silkworm is fed with mulberry leaves or artificial feed, and the feeding conditions are: temperature 25°C, relative humidity 75% ± 5%, photoperiod 12 hours of light + 12 hours of darkness. The silkworm samples from the middle silk gland on the third day of the fifth instar and the silkworm on the seven...
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