Method for producing heavy metal absorbent through microbiological treatment on lignite and related compound microbial agent
A technology of composite microbial agent and heavy metal, applied in the field of resource utilization of lignite, can solve few problems and achieve the effect of improving capacity
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Embodiment 1
[0024] The preparation of embodiment 1 fermented liquid
[0025] In this example, Beauveria bassiana was purchased from ATCC, numbered ATCC7159; Bacillus subtilis was purchased from ACCC, numbered ACCC19374; Candida tropicalis was purchased from ATCC, numbered ATCC20326.
[0026] (1) Activate the above-mentioned Beauveria bassiana (ATCC7159), Bacillus subtilis (ATCC11775), and Candida tropicalis (ATCC20326) into their respective slant media for preservation, and culture them at 26-37°C for 16-72 hours.
[0027] Preservation of three kinds of microorganisms uses the slant culture medium formula as follows:
[0028] The medium used for Beauveria bassiana: 200 grams of potato, 20 grams of glucose, 10 grams of peptone, 15 grams of agar, 1000 ml of tap water, natural pH;
[0029] Medium for Bacillus subtilis: 10 grams of peptone, 5 grams of glucose, 3 grams of beef extract, 5 grams of sodium chloride, 15 grams of agar, 1000 ml of tap water, pH7.2±0.2;
[0030] The medium used for...
Embodiment 2
[0035] Example 2 Adsorption of copper-containing test solution after processing lignite with composite microbial bacterial agent
[0036] 1. Select 8kg of lignite coal powder (moisture content 20.0%) that has been crushed through a 80 mesh sieve, divide it into 4 parts on average, add in 4 20L reactors respectively, add 150g of soybean meal hydrolyzate (Shandong Yangcheng Biotechnology Co., Ltd.) and A total of 50g of urea, 100g of glucose, adding water to adjust the moisture content of the material to 90%, adjust the pH to 7.0, and the total volume is 16L;
[0037] 2, three kinds of microbial fermented liquids prepared in embodiment 1 are combined into composite microbial inoculum by following parts by weight: 20 parts by weight of Beauveria bassiana fermented liquid, 9 parts by weight of Bacillus subtilis fermented liquid, 8 parts by weight of Candida tropicalis fermented liquid parts by weight;
[0038] 3. Add 48ml each of the following microbial fermentation broth or micr...
Embodiment 3
[0044] Example 3 Adsorption of lead-containing test solution after processing lignite with composite microbial bacterial agent
[0045] 1. Select 12kg of lignite coal powder (moisture content 28.6%) that has been crushed through a 80-mesh sieve, divide it into 4 parts on average, add them to 4 20L reactors, then add 150g of peptone and 210g of glucose, and add water to adjust the moisture content of the material to 88%. , adjust the pH to 6.8, and the total volume is 17.9L;
[0046] 2. Prepare three kinds of microbial fermented liquids according to the method of Example 1, and mix uniformly by the following parts by weight to become composite microbial inoculum I: 19 parts by weight of Beauveria bassiana fermented liquid, 10 parts by weight of Bacillus subtilis fermented liquid, tropical pseudo 7 parts by weight of fermented liquid of Trichosomyces; mix uniformly according to the following parts by weight to form composite microbial agent II: 4 parts by weight of Beauveria bas...
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