62 results about "Dryopteris fragrans" patented technology

The invention discloses a fluoro methoxylpyrazole-containing o-formylaminobenzamide compound shown as a structural formula (I) or agriculturally suitable salts thereof, a synthesis method and application thereof. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has the advantages of effectiveness, low toxicity, low cost and environmental safety and can be used for preventingand removing various pests in the fields of agriculture and horticulture or pests parasitized on animals. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has very high activityon lepidopteran pests (plutella xylostella, spodoptera exigua and cotton bollworm), hemipteran pests (black peach aphid and aphis craccivora koch), homopteran pests (nilaparvata lugens), dipteran pests (liriomyza trifolii) and leaf beetle pests (phaedon conchleariae). Particularly, good effect on the lepidopteran pests, the hemipteran pests and the dipteran pests can be achieved under the condition of low dosage of the compound.

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

The invention discloses the separation, clone and application of insecticidal crystal protein gene from Bacillus thuringiensis. In this invention a novel insecticidal crystal protein cry7Ba1 is separated from Bacillus thuringiensis Central China species YBt-978 strains. The genes code a novel insecticidal crystal protein Cry7Ba1 with insecticidal activity to Lepidoptera insects. Cry7Ba1 protein is consisted by 1,154 amino acid, and its homology with the existing published proteins is no more than 80% and the half of amino acid sequence near the protein N-terminal has no more than 50% homology with the existing published proteins. The invention also discloses the gene sequence of this novel insecticidal crystal protein and its coding protein Cry7Ba1 expressed by microorganism transformed by appropriate expression vector, qualifying it with lepidoptera insecticidal activity.

The invention provides a mitochondrial gene sequence for identifying authenticity and quality of Cordyceps sinensis and an operation method thereof. According to the method, differences of mitochondrial genes between ghostmoth and other lepidopteran insects are compared and thus high-variability areas can be searched. Specific primers of the ghostmoth are designed to obtain sequences of corresponding sections of mitochondria of host insects; if a nucleotide difference rate is less than 1-1.5 %, the insects are determined to be a same species but different individuals, and if the nucleotide difference rate is more than 6 %, the insects are determined to be a same genus but different species, thereby not only identifying whether to-be-detected samples are the Cordyceps sinensis formed with Hepialus larvae as the host insects, but also determining producing areas of the to-be-detected samples, and thus determining the authenticity and grades of the to-be-detected samples The method is simple in steps, easy to operate, and few in sample amount, and can minimize sample loss. According three pairs of the specific primers provided by the invention, the authenticity and the grades of the samples can be rapidly and conveniently detected through detecting whether the samples have same or similar nucleotide sequences as well as similarity of the nucleotide sequences.

The invention discloses a Bacillus thuringiensis with activity on resisting Bacillus thuringiensis (Bt) plutella xylostella, and relates to a Bacillus thuringiensi. The Bacillus thuringiensis is Bacillus thuringiensis AH-2 and belongs to the Bacillus, and is preserved in the China General Microbiological Culture Collection Center with the collection number of CGMCC No: 5220 in September 6, 2011. The Bacillus thuringiensis AH-2 has strong action on poisoning and killing Lepidoptera and Coleoptera main pests and resisting Bt and Cry1Ac toxalbumin pests. The Bacillus thuringiensis can be used for producing efficient broad-spectrum insecticide, can be used for preventing the Lepidoptera important pests such as plutella xylostella and the Coleoptera pest such as yellow mealworm, has good effect on resisting pests and is favorable for preventing and controlling field resistance plutella xylostella.

The invention discloses a microwave extraction method for dryopteris fragrans(L.)schott essential oil. The optimum microwave extraction process is selected by controlling the sizes and water content of particles of dryopteris fragrans(L.)schott powder, the microwave intensity is set to be 100 to 500W, the extraction time is 10 to 50 minutes, and the high-quality dryopteris fragrans(L.)schott essential oil can be obtained. The method is easy to operate, and low in production cost; and the prepared product is high in activity and yield. The dryopteris fragrans(L.)schott essential oil prepared by the method has golden yellow color; and cosmetics which are prepared from extractive have the effect of resisting corynebaterium acnes and an obvious anti-aging effect.

The invention clones insect specific neurotoxin gene BmkIT of East Asian scorpion and insect chitinase gene Chi to an insect cell-converting medium carrier pAcPDIEI to obtain a recombinant plasmid containing divalent anti-insect genes pAcPDIEI-Bmkit-Chi and transfecting the recombinant plasmid and DNA of AcNPV genome together in sfg cells, and obtaining a recombinant rhabdovirus containing divalent anti-insect genes AcNPV-BmKIT-Chi. The cooperation of the insect specific neurotoxin with the chitinase improves the poisoning effect of virus on cotton bollworms and other lepidopteral pests.

Novel Bacillus thuringiensis genes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-active B. thuringiensis microbes. The DNA encoding the B. thuringiensis toxins can be used to transform various prokaryotic and eukaryotic microbes to express the B. thuringiensis toxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

The invention discloses application of a compound for efficient inhibition of chitinase OfChi-h and hexosaminidase OfHex1 activity, and relates to application of a compound phlegmacin B1 and its derivatives in inhibiting chitinase OfChi-h and hexosaminidase OfHex1 activity. The used final concentration is not less than 13ppm, under the concentration, the measured inhibition rates are 90.4% and 71.4% respectively, and inhibition constants Ki are 5.5 microM and 26 microM respectively. The invention proves that the compound phlegmacin B1 has good application prospect in the prevention and control of agricultural pests, especially in prevention and control of lepidoptera insects.

The invention discloses an induction method and application of dryopteris fragrans callus tissues, and belongs to the technical field of biology. The method provided by the invention comprises the following steps: preparing dryopteris fragrans spores into a sterile spore suspension, inoculating the sterile spore suspension to improved Knop' s culture mediums, performing dark treatment, and after germination, performing culturing so as to obtain sporophytes; using stem tips and young and tender leaf bases of the obtained dryopteris fragrans sporophytes as explants, inoculating the explants to culture mediums in which 6-BA and NAA are added, and performing culturing so as to obtain green spherules; inoculating stem tips of the green spherules or the stem tips of the sporophytes into culture mediums containing 6-BA and 2,4-D, and performing proliferation and differentiation so as to obtain the callus tissues. The method disclosed by the invention lays the theory and practice foundation for establishing a rapid propagation system of dryopteris fragrans, so that wild resources of the dryopteris fragrans are protected, and the blank of performing callus tissue induction through stem tips and young and tender leaf bases in the tissue culture research of the dryopteris fragrans is filled.

Disclosed is a novel cadherin peptide that enhances the toxicity of Cry proteins. A novel insecticide composition comprising an effective amount of cadherin peptide having SEQ. ID. NO:2 and an effective amount of Bacillus thuringiensis Cry protein wherein the cadherin peptide comprises a Cry3Aa toxin binding region from the full-length T. molitor cadherin and has synergistic characteristics of a binary toxin potentiating Cry3 and Cry1 toxins against coleopterans and lepidopteran species, respectively

The invention discloses a pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The protein comprises an amino acid sequence shown as SEQ ID NO: 2. The invention further discloses genes coding the pteromalus puparum venom Kazal-type serine protease Inhibitor PpSPI20 protein. The genes comprise a nucleotide sequence at sites 70-246 in SEQ ID NO:1. The inhibitor disclosed bythe invention can be used for inhibiting activation of hemolymph PPO (Poly Phenylene Oxide) of imported cabbageworms or papilio xuthus. The invention further discloses a method for improving prevention ability of brassicaceous vegetables on lepidoptera pests. The method comprises the following steps: transforming brassicaceous vegetable cells by using the genes comprising the nucleotide sequenceshown as SEQ ID NO:1, and cultivating the transformed brassicaceous vegetable cells into plants.

The invention relates to a beauveria bassiana strain having insect intestinal infection effect, and an application thereof in pest prevention and treatment. In particular, the invention relates to beauveria bassiana which is assigned the accession number of CGMCC No.13180, or spores, mycelia or a fermented product of the beauveria bassiana, or a composition including the strain or the spores thereof, as well as a preparation method thereof, and application of the products in pest prevention and treatment. Herein, the strain and the composition have synergistic effect in pest prevention and treatment (e.g. lepidoptera and dipteran insects) in a unique manner of intestinal infection and intestinal / integument dual-infection, so that the strain has extensive application prospect.

The invention discloses a cordyceps militaris culture medium. The culture medium adopts water as a solvent. The obtained cordyceps militaris culture medium is prepared from, by weight, 30-70 parts ofmain materials, 5-20 parts of lepidopteron powder, 3-12 parts of glucose, 2-8 parts of peptone, 1-5 parts of corn starch, 0.5-5 parts of ammonia citrate, 0.5-5 parts of magnesium sulfate, 0.5-5 partsof monopotassium phosphate, 7-12 parts of hydroxyethyl cellulose and 15-30 parts of traditional Chinese medicine additives. The traditional Chinese medicine additives include, by weight, 40-55% of tribulusterrestris, 20-40% of aloe leaves and 5-25% of astragalus membranaceus. The invention further discloses a preparation method of the cordyceps militaris culture medium. The cordyceps militaris culture medium obtained according to the preparation method can effectively restrain infectious microbe growth, accelerate hypha forming, enhance cordyceps militaris sporocarp oxidation resistance and have the wide application prospect.

The invention belongs to the technical field of microbial insecticides and particularly relates to bacillus thuringiensis YN108 with high toxicity to lepidopteran pests, a culture method and application of the bacillus thuringiensis YN108. The bacillus thuringiensis YN108 is bacillus thuringiensis subsp.aizawai and has a preservation number of CGMCC No. 15140 in the China General Microbiological Culture Collection Center (CGMCC) on January 02, 2018. The bacillus thuringiensis YN108 dislcosed by the invention has high toxicity to the lepidopteran pests, and particularly has a median lethal concentration (LC500) of only 3.25*105 cfu / ml for the 3<rd> instar larva of spodoptera litura, which is smaller than a published YN1-1 bacterial strain and a published CAB109 bacterial strain. Meanwhile,the bacillus thuringiensis YN108 is also better than a commercial bacillus thuringiensis catfish subspecies I product, an SB product and a bacillus thuringiensis catfish subspecies SL product in indoor drug efficacy and field control effect and shows excellent insecticidal activity. In addition, the bacillus thuringiensis YN108 disclosed by the invention has no residue after use, avoids drug resistance and is green and environmentally-friendly.

Novel Bacillus thuringiensis genes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-active B. thuringiensis microbes. The DNA encoding the B. thuringiensis toxins can be used transform various prokaryotic and eukaryotic microbes to express the B. thuringiensis toxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

The invention relates to the field of pesticides, in particular to a pesticide containing copperleaf herb and derris elliptica extracts, and a preparation method and application thereof. Active ingredients of the pesticide containing the copperleaf herb and derris elliptica extracts are extracted from 1 weight part of copperleaf herb and 1 to 8 weight parts of derris elliptica. The pesticide containing the copperleaf herb and derris elliptica extracts has a synergistic effect, has an obvious effect of controlling Lepidoptera pests, can strengthen immune systems of plants while quickly killing the pests, and can obviously improve plant vitality; the pesticide is prepared from Chinese herbal medicines, so the problem of pesticide residue is solved and the pests hardly generate resistance; during use, the effective concentration is low and the cost is low.

The invention discloses a Wudalianchi dryopteris fragrans mineral spring effervescent tablet and a preparation method thereof, and relates to a dryopteris fragrans mineral spring effervescent tablet mainly used for solving the problems of existing Wudalianchi dryopteris fragrans which is insufficient in active ingredient extraction, is incapable of effectively developing the efficacy and is poor in application effect, and the problems that existing bath supplies which cannot effectively improve skin diseases caused by bacterial and fungal infection and antibiotic and corticoid bath supplies can cause serious injury to body and skin. The effervescent tablet is prepared from the following raw materials in parts by mass: 5-30 parts of a Wudalianchi dryopteris fragrans extract, 5-20 parts of a Wudalianchi heavy-carbonate mineral spring extract, 5-20 parts of Wudalianchi volcanic mud powder, 5-50 parts of an acid source, 5-50 parts of an alkali source, 2-20 parts of a lubricant, 0.1-50 parts of filler and 0.1-5 parts of essence. The preparation method comprises the following steps: extracting Wudalianchi dryopteris fragrans, mineral spring extract and Wudalianchi volcanic mud powder, and mixing the extracted Wudalianchi dryopteris fragrans, mineral spring extract and Wudalianchi volcanic mud powder with the acid source, the alkali source, the lubricant and the filler so as to obtain the effervescent tablet.

The invention relates to a bacillus thuringiensis insecticidal gene cry79Aa1, an expression protein and application thereof and belongs to the technical field of biological control. The insecticidal protein Cry79Aa separated from bacillus thuringiensis LTS290 (preseravtion number: CGMCC No.10232) has an amino acid sequence shown as SEQ ID NO.2, and a gene encoding the insecticidal protein, preferably the nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene has certain toxicity to lepidopteran pests, so that the gene can be applied to transformation of microobes and plants, can show toxicity to related pests, and can overcome and delay the generation of drug resistance of the pests to engineering bacteria and transgenic plants.

The invention relates to the technical field of medicine and discloses an antibacterial application of a Dryopteris fragrans phloroglucinol compound flavaspidic acid BB. The invention provides the flavaspidic acid BB obtained by the chemical synthesis method. The flavaspidic acid BB has good antibacterial effects, effectively fills in the application deficiency of the antibacterial natural compound and provides an effective antibacterial solution for drug-resistant bacteria. The experimental results show that the compound has a strong antibacterial effect and good curative effects especially on drug-resistant bacteria.

The present invention relates to the technical field of medicines and discloses dryopteris fragrans phloroglucinol derivatives, a separation and preparation method for isomers thereof and an application thereof Phloroglucinol compounds are separated and prepared from dryopteris fragrans(L.)Schott, which belongs to dryopteridaceae dryopteris plants, for the first time, and 4 pairs of isomers are discovered, wherein the 4 pairs of isomeric compounds have relatively strong effect of resisting skin superficial fungi. According to the in-vitro bacteriostatic experiment, the compounds provided by the present invention have good bacteriostatic effect on two skin pathogenic fungi, namely trichophyton rubrum and microsporum gypseum, and have characteristics of being low in side effect and being not liable to become resistant to the drug, and the like, thereby providing a novel natural compound for the treatment of skin superficial fungi infection and providing powerful technical support for preparing anti skin superficial fungi infection drugs or pharmaceutical compositions.

The invention discloses a pteromalus puparum venom Kazal-type serine protease inhibitor protein PpSPI24 with an amino acid sequence as shown in SEQ ID NO:2. The invention further discloses a gene forcoding the chrysalis nasonia venom Kazal-type serine protease inhibitor protein PpSPI24, and the gene has nucleotide sequences of sites 70-231 in SEQ ID NO:1. The inhibitor can be used for inhibitingactivation of imported cabbageworm or papilio xuthus linnaeus hemolymph PPO. The invention further discloses a method of improving prevention force, on lepidoptera pests, of brassicaceous vegetables,and the method comprises the following steps: converting cells of the brassicaceous vegetables by the gene with the nucleotide sequences as shown in SEQ ID NO:1; and cultivating the converted cells ofthe brassicaceous vegetables into plants.

The invention discloses bacillus thuringiensis for inhibiting stem bleeding disease of coconut and killing lepidoptera pests and application thereof. The bacillus thuringiensis has a collection number of CGMCC No.7967. A bacillus thuringiensis strain WENCHANG-BT20 is directly separated and screened from a soil sample on the coast of Hainan and carries four insecticidal crystal protein encoding genes of crylAa, crylBe, cryllia and cry2Aa. The bacillus thuringiensis has high insecticidal capacity and good insecticidal effect and has a certain effect of inhibiting fungal diseases.

The invention relates to a multifunctional composite deterging liquid, comprising cocoamidopropyl betaine, sodium dodecyl diphenyl ether disulfonate, Herba Houttuyniae extract, Dryopteris fragrans extract, sodium glycyrrhizinate, methylglycine proline, 2-amino-2-ethyl-1-pentanol, 2-methylbutyrate-hexa-5-ene ester, soap of fatty acid potassium, polyepoxysuccinic acid and water.The multifunctional composite deterging liquid shows excellent performances such as antibiosis, detergence, softness and smoothness, aroma enhancement and low foaminess, meets the consumption needs of people and has a promising industrial application prospect.

The invention discloses an anti-allergic plant composition and application thereof and belongs to the technical field of cosmetics. The composition comprises Dryopteris fragrans extract, radix scutellariae extract, opuntia extract and radix glycyrrhizae extract. The anti-allergic plant composition has the advantages that the raw materials used by the composition are mild natural components and contain rich substances such as amino acid and trace elements capable satisfying human body physiological needs and anti-allergic substances; the anti-allergic plant composition can be prepared into three skincare products which include a facial cleanser, a toner and a moisturizing lotion according to the needs and convenience of users, and the users can select and use the skincare products according to the needs.

The invention discloses a method for extracting aspidinol from blocks. Dryopteris fragrans (L.)Schott is taken as an original material, and a method for quickly extracting and separating the aspidinol is found; the extracting time can be shortened to 4-10 hours; and a foundation is laid for wide application of the Dryopteris fragrans (L.)Schott.

The invention discloses Bacillus bombyseptieus parasporal crystal toxin (Bb toxin) protein and a truncated segment thereof as well as a recombinant expression vector and an application of the Bacillus bombyseptieus parasporal crystal toxin protein. The amino acid sequence of the Bacillus bombyseptieus parasporal crystal toxin protein is shown by SEQ ID NO.4, and the truncated segment of the Bacillus bombyseptieus parasporal crystal toxin protein is shown by SEQ ID NO.8. The survival rate of domestic silkworms is obviously reduced (about 30%) after silkworm larvae are additionally fed with the truncated segment, which shows that the gene can be used for coding a toxic protein capable of causing lepidopteron, namely the domestic silkworms to death, and the Bacillus bombyseptieus parasporal crystal toxin protein can be used for preparing biological pesticides for resisting lepidoptera pests and has wide application prospect.

The invention discloses a pinellia ternate trypsin inhibitor and its use in pest resistance. The pinellia ternate trypsin inhibitor belongs to the technical field of biochemistry and molecular biology. The pinellia ternate trypsin inhibitor has an amino acid sequence shown in the formula of SEQ ID NO1 in the sequence table. The invention also discloses an active mutant of the pinellia ternate trypsin inhibitor. The pinellia ternate trypsin inhibitor has obvious effects of killing lepidopteron and hemipterous pests.

The invention discloses a trypsin inhibitor gene with insecticide resistance in populus nigra and application thereof. The trypsin inhibitor gene with insecticide resistance in populus nigra is shown as SEQ ID NO.1 or SEQ ID NO.2 in a sequence table; populus nigra Kunitz type trypsin inhibitor gene (PnKTIs) separated in the invention has high inductivity to two external inducing stimulations, namely mechanical damage and insect bite. Experiments prove that: the trypsin inhibitor gene has insecticide resistance, and the survival rate of lepidopteran insects is remarkably reduced. According to the trypsin inhibitor gene with insecticide resistance in populus nigra and the application thereof, a novel insecticide-resistance gene is provided to related searchers working on insect-resistant transgenic engineering and biological control, and a novel insecticide resistance means is developed on the basis of the application, pesticide use and vicious pollution to the environment are reduced, and balance of an ecological system and sustainable development are maintained.