38 results about "Nojirimycin" patented technology

The invention discloses a method for extracting a deoxynojirimycin monomer. The method comprises the following steps of: soaking a mulberry raw material in acidic aqueous solution, and extracting for 1 to 3 times; adsorbing extracting solution on a cation exchange resin, washing, and eluting by using ammonia water; performing reverse osmosis pre-concentration on eluent, recovering ammonia, dissolving an intermediate in a hydrophilic solvent, performing catalytic conversion, allowing the solution to pass through a gel chromatographic column, and eluting the hydrophilic solvent; recovering the solvent, dissolving in a mixed solvent, allowing the mixed solvent to pass through the chromatographic column, and eluting the mixed solvent; and recovering the mixed solution to obtain pure deoxynojirimycin products. The method is low in energy consumption and environment-friendly, and the pure deoxynojirimycin products with various purity grades of 10 percent, 50 percent, 99 percent and the like.

The invention provides a preparation method of mulberry leaf extract containing 1-deoxynojirimycin. The method mainly including the steps of saline solution water-proof cooking and softening, enzymolysis, polyamide column purification and simulated moving bed refining is determined by sufficiently considering the raw material characteristics of mulberry leaves and the dissolution characteristics of the target component 1-deoxynojirimycin and conducting system screening and optimizing. The separation effect is extremely good, and the concentration of 1-deoxynojirimycin in the finally obtained mulberry leaf extract is larger than 95 wt%. All materials used for the whole method are conventional materials, cost is low, yield is high, solvent can be recycled, environment pollution can be reduced, and industrialization can be easily achieved.

The invention discloses an oral preparation for slowing down the absorption of an alpha-glycosidase inhibitor and enhancing a hypoglycemic drug effect. The preparation is an oral preparation prepared by adding the following adhesive auxiliary materials based on the certain proportion: carboxymethyl cellulose, hydroxyethyl cellulose, methyl cellulose or beta-cyclodextrin and the like, to each alpha-glycosidase inhibitor (acarbose, voglibose, miglitol, 1-deoxynojirimycin and extract of the 1-deoxynojirimycin). Compared with singly taken alpha-glycosidase inhibitors, the adhesives can be used for prolonging the action time of the alpha-glycosidase inhibitor in the intestine, slowing down the absorption of a medicament and glucose in the intestine and further reducing a plasma concentration, thus the hypoglycemic drug effect is improved. The oral preparation disclosed by the invention can be applied to the prevention and therapy of diseases, such as diabetes, obesity and the like.

The invention provides a method for extracting 1-deoxynojirimycin (DNJ) from mulberry leaves, which is obtained by adding inorganic acid to the extract of mulberry leaves for acidification, cooling to remove impurities, and then obtaining through column chromatography. The content of mulberry leaf extract can be no less than 10%. The mulberry leaf extract obtained by the method of the invention has been proved by animal experiments that it has no effect on normal mice, but has a relatively obvious hypoglycemic effect on diabetic model mice, and can be used in the preparation of health products for reducing blood sugar. Since the method of the present invention does not need to add flocculants, it can reduce the cost and increase the content while ensuring that no exogenous substances are brought in during the post-treatment process. After the acidification solution is concentrated, it is directly separated by domestic ion exchange column chromatography. The cost is greatly reduced. The method of the invention is reasonable in design, easy to operate, does not need special equipment, and is very suitable for industrial production.

The invention discloses a preparation method and application of a healthy food base material capable of improving metabolic disturbance of glycolipid. The preparation method comprises the following steps of picking mulberry leaves, and performing pretreatment; preparing fresh mulberry leaf virgin pulp; preparing mulberry leaf acidic polysaccharose; preparing a tartarian buckwheat extract; preparing an oat extract; preparing composite virgin pulp; performing sterilization and fermentation; and performing drying, packaging and sterilization. The healthy food base material is rich in gamma-aminobutyric acid, N-saccharide compounds (1-deoxidized nojirimycin, fagopyrin and the like), micromolecular oligosaccharide prebiotics and probiotics. According to the method, on one hand, the content of the gamma-aminobutyric acid and the content of the N-saccharide compounds in raw materials are increased through optimizing microorganism fermentation strains and a fermentation technology, besides, conversion of natural macromolecular polysaccharide in the raw materials into the oligosaccharide prebiotics can also be promoted in the fermentation process, and on the other hand, the bioactivity of functional factors of the gamma-aminobutyric acid, the N-saccharide compounds, the micromolecular oligosaccharide prebiotics, the probiotics and the like in products can be reserved to the maximum extent through optimizing a preparation technology.

The invention relates to the fields of medicine and food, in particular to 1-deoxynojirimycin-hydroxychalcone hybrid derivatives and their preparation methods and applications. 1-deoxynojirimycin-hydroxychalcone hybrid derivatives have a structure shown in formula (I), and it has been proved by in vitro tests that this type of compound is a potent inhibitor of α-glucosidase. The inhibitory activity is significantly higher than that of acarbose, the most commonly used postprandial blood glucose control agent at present. Among them, the activities of the two compounds DC‑5 and DC‑2 are also significantly higher than 1‑deoxynojirimycin. Since these compounds can strongly inhibit the activity of sugar-digesting enzymes such as α-glucosidase, and can effectively reduce postprandial blood sugar, they have very good application prospects in hypoglycemic drugs and auxiliary hypoglycemic foods.

The invention provides a method for detecting the content of alpha-homonojirimycin in a suregade glomerulata medicinal material. The method comprises the following steps: preparing a reference substance solution, namely, precisely weighing an alpha-nojirimycin reference substance, and adding a solvent for dissolving to obtain the reference substance solution with required concentration; preparinga test solution, namely, taking suregade glomerulata medicinal material powder to be detected, adding an extraction solvent for extracting, carrying out centrifuging, taking a supernatant, and fixingthe volume to obtain the test solution; and carrying out content determination, namely, determining the peak areas of the alpha-homonojirimycin in the reference substance solution and the test solution through a liquid chromatography-tandem mass spectrometry method, and calculating the content of the alpha-homonojirimycin in the suregade glomerulata medicinal material according to an external standard method. According to the method, a liquid chromatography-tandem mass spectrometry method is adopted, resolution of the polyhydroxy alkaloid substance alpha-homonojirimycin and the four chiral isomers of the polyhydroxy alkaloid substance alpha-homonojirimycin is achieved for the first time, direct qualitative and quantitative analysis of the alpha-homonojirimycin is achieved, and the method has good system applicability, stability, repeatability and reproducibility.

The invention discloses a method for extracting a deoxynojirimycin monomer. The method comprises the following steps of: soaking a mulberry raw material in acidic aqueous solution, and extracting for 1 to 3 times; adsorbing extracting solution on a cation exchange resin, washing, and eluting by using ammonia water; performing reverse osmosis pre-concentration on eluent, recovering ammonia, dissolving an intermediate in a hydrophilic solvent, performing catalytic conversion, allowing the solution to pass through a gel chromatographic column, and eluting the hydrophilic solvent; recovering the solvent, dissolving in a mixed solvent, allowing the mixed solvent to pass through the chromatographic column, and eluting the mixed solvent; and recovering the mixed solution to obtain pure deoxynojirimycin products. The method is low in energy consumption and environment-friendly, and the pure deoxynojirimycin products with various purity grades of 10 percent, 50 percent, 99 percent and the like.

The invention relates to the preparation of tea and aims to provide a preparation method of ultramicro mulberry leaf tea powder. For obtaining ultramicro mulberry leaf powder and ultramicro tea powder, the method comprises the following steps of: selecting raw materials, killing out, kneading, drying, primarily crushing and ultromicro crushing; sufficiently mixing ultramicro mulberry leaf powder with a nojirimycin crude product with a V type mixer, and sufficiently mixing the mixture with ultramicro tea powder to obtain the ultromicro mulberry leaf tea powder, wherein the weight content of nojirimycin in the nojirimycin crude product is 10-12 percent; and finally packing. For the ultromicro mulberry leaf tea prepared by the invention, ultromicro crushed to obtain the grain size less than 10 mum, the powder can be sufficiently suspended in hot water and can be completely taken, thereby the health care and nutritious value of the mulberry leaf are fully exerted. The nojirimycin crude product is added in the ultramicro mulberry leaf tea powder so that the activity of alpha-glucosidase can be more effectively restrained, glucose entering the blood is reduced, and the blood sugar is reduced. The invention can be used for health care or auxiliarily treating diabetes.

The invention relates to a technical method for separating monomers with hypoglycemic alkaloid activity such as fagopyrin, 1,4-dideoxy-1,4-imino-d-arabinitol and 1-deoxynojirimycin from white mulberry root-bark total alkaloid. The method comprises the following steps: by using the self-extracted white mulberry root-bark total alkaloid as a primary material, re-dissolving through methyl alcohol to remove most of DNJ (1-deoxynojirimycin); concentrating eluent, then performing derivatization reaction, performing column chromatography through a silica gel column, obtaining multiple active alkaloid monomers through column elution, and finally, dissociating derivatized groups to obtain the alkaloid monomers. The content of the obtained monomers exceeds 95.0 percent, and the monomers can be used as standard reference materials or other materials.

The invention relates to the technology field of effective ingredient separation of the Chinese traditional medicine, particularly a method for separating 1-desoxy nojirimycin monomer from total alkaloid of mulberry leaf. The method comprises the steps as follows: preparing a sample solution; pretreating adsorbent and packing into a column; passing through the resin column at the room temperature; collecting eluates part by part; subjecting to the qualitative detection; mixing the eluates with the same composition; concentrating under the reduced pressure; vacuum-drying; and recrystallizing to obtain 1-desoxy nojirimycin crystals. According to the high-efficiency liquid chromatography measurement, the purity of the crystal is higher than 95% and the yield of the crystal is higher than 75%.

The invention relates to a preparation method of miglitol intermediate, in particular to a preparation method of a derivative of N-substitution-1-deoxynojirimycin which is the key intermediate of miglitol. Gluoconobacter oxydans or the Gluoconobacter oxydans treated by immobilized cell technology are used repeatedly for transforming the substrate to the derivative of N-substitution-1-deoxynojirimycin which is the miglitol intermediate, the transfer temperature is controlled at 5 to 25 DEG C, pH value is controlled at 4.0 to 6.5 and dissolved oxygen is controlled at 5 to 80 percent (in volume). Adopting the invention can decrease the biotransformation cost, reduce the inter-assay of cell, and be suitable for the large scale industrial production, increase the mechanical strength of the wall of the strain prepared, make the strain not to dissolve during the cultivation in long-term liquid environment, lengthen the service life and have outstanding slow release performance.

Novel pro-drugs and methods for their use to alter the growth and biological characteristics of living cells, tissues, or whole organisms are described. Such cells may include animal cells, plant cells and bacterial cells. The methods allow for selective activation of the pro-drugs at or near transformant host cells expressing a gene for an enzyme that activates the pro-drugs. Pro-drugs according to a preferred embodiment of the invention are conjugates of a bioactive compound and a chemical group that is capable of being cleaved from the bioactive compound by action of an enzyme. Methods according to this invention include, (a) introducing into targeted cells a gene encoding an enzyme and (b) administering a pro-drug, wherein the enzyme releases the pro-drug from conjugation. In a preferred embodiment of the invention, the gene encoding the enzyme is a marker gene. Methods according to a preferred embodiment of the invention include altering protein production in a plant cell such as altering glycosylation of a protein produced by the plant cell.

The invention discloses a preparation method and application of a health food base material capable of improving glucose and lipid metabolism disorders, comprising the following steps: picking and pretreatment of mulberry leaves; preparation of fresh mulberry leaf puree; preparation of mulberry leaf acidic polysaccharide; preparation of tartary buckwheat extract ; Preparation of oat extract; Preparation of compound puree; Sterilization and fermentation; Drying, packaging and sterilization. The health food base material of this invention is rich in γ-aminobutyric acid, N-saccharide compounds (1-deoxynojirimycin and buckwheat alkali, etc.), small molecule oligosaccharide prebiotics and probiotics; Optimize the microbial fermentation strain and fermentation process to increase the content of γ-aminobutyric acid and N-saccharide compounds in the raw material, and at the same time promote the conversion of natural macromolecular polysaccharides in the raw material into oligosaccharide prebiotics during the fermentation process. On the one hand, the biological activity of functional factors such as γ-aminobutyric acid, N-saccharide compounds, small molecular oligosaccharides, prebiotics and probiotics can be retained to the greatest extent through optimization of the preparation process.