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Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material

A technology of konojirimycin and a detection method is applied in the field of content detection of α-konojirimycin in white tree medicinal materials, and can solve the problems of high risk, insufficient resolution, cumbersome reaction procedures, etc. The effect of good system suitability

Active Publication Date: 2020-04-17
CHINA RESOURCES SANJIU MEDICAL & PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of inaccurate detection results caused by the cumbersome reaction procedures, high risk and insufficient resolution ability of the content detection method of α-konojirimycin in the prior art, thereby providing a Determination method of α-takanojirimycin in the medicinal material of Baishu with simple operation, rapidity, environmental friendliness and ability of isomer resolution

Method used

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  • Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material
  • Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material
  • Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material

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Embodiment 1

[0059] A method for detecting the content of α-konojirimycin in Radix Radix Medicae, the specific operations are as follows:

[0060] (1) Preparation of reference substance solution: take 150 mg of α-konojirimycin reference substance, accurately weigh it, transfer it to a 50mL volumetric flask, add ethanol solution with a volume concentration of 60% to dissolve, and constant volume to obtain a stock solution. Dilute to a concentration of 3 μg / mL, and filter through a 0.22 μm organic filter membrane to obtain the reference substance solution;

[0061] (2) Preparation of the test solution: take 1.0 g of the white tree medicinal material powder to be tested (passed through a No. 4 sieve), accurately weigh it, place it in a stoppered conical flask, and accurately transfer it into 100 mL of ethanol solution with a volume concentration of 60%. Ultrasonic extraction at 50°C for 50 minutes, let it cool, supplement the lost weight with ethanol solution with a volume concentration of 60...

Embodiment 2

[0075] A method for detecting the content of α-takanojirimycin in the Radix Gelvinia medicinal material, the specific operation is the same as that in Example 1, the difference is that in step (2): the extraction solvent is ultrapure water, and Gelbelina is added before the ultrasonic extraction The mass volume ratio of medicinal material powder to extraction solvent is 0.5:100g / mL, and the ultrasonic extraction condition is ultrasonic extraction at 40°C for 50min; in liquid chromatography conditions, column temperature: 20°C.

Embodiment 3

[0077] A method for detecting the content of α-konojirimycin in Radix Radix Medicinae, the specific operation is the same as in Example 1, the difference is that in step (2): the extraction solvent is an ethanol solution with a volume concentration of 20%, and ultrasonic extraction The conditions are ultrasonic extraction at 60°C for 50min; in liquid chromatography conditions, flow rate: 0.5mL / min, column temperature: 50°C.

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Abstract

The invention provides a method for detecting the content of alpha-homonojirimycin in a suregade glomerulata medicinal material. The method comprises the following steps: preparing a reference substance solution, namely, precisely weighing an alpha-nojirimycin reference substance, and adding a solvent for dissolving to obtain the reference substance solution with required concentration; preparinga test solution, namely, taking suregade glomerulata medicinal material powder to be detected, adding an extraction solvent for extracting, carrying out centrifuging, taking a supernatant, and fixingthe volume to obtain the test solution; and carrying out content determination, namely, determining the peak areas of the alpha-homonojirimycin in the reference substance solution and the test solution through a liquid chromatography-tandem mass spectrometry method, and calculating the content of the alpha-homonojirimycin in the suregade glomerulata medicinal material according to an external standard method. According to the method, a liquid chromatography-tandem mass spectrometry method is adopted, resolution of the polyhydroxy alkaloid substance alpha-homonojirimycin and the four chiral isomers of the polyhydroxy alkaloid substance alpha-homonojirimycin is achieved for the first time, direct qualitative and quantitative analysis of the alpha-homonojirimycin is achieved, and the method has good system applicability, stability, repeatability and reproducibility.

Description

technical field [0001] The invention relates to the field of content detection of traditional Chinese medicinal materials, in particular to a content detection method of α-konojirimycin in white tree medicinal materials. Background technique [0002] White tree, known in Latin as Suregade glomerulata (BL.), belongs to the Euphorbiaceae white tree genus, arbor, 2-13 meters high, with gray-black or brown twigs. Existing studies have proved that the extract of white tree can specifically inhibit α-glucosidase and has strong activity, which is beneficial to the improvement of glucose metabolism and lipid metabolism disorders in diabetes, and can be used for the treatment of diabetes and abnormal glucose tolerance. Azasaccharide-type polyhydroxyalkaloids represented by α-takanojirimycin structural core are natural α-glucosidase inhibitors with hypoglycemic activity extracted and isolated from white tree, which have important development value. However, due to the characteristics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 徐冰王勇韩晓强吴正军王洪庆代志李琼娅李文艳袁勤芬段娟慧马鹏岗
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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