46 results about "N-Glycosylation Site" patented technology

The present invention relates to novel polypeptide variants of factor VII (FVII) or factor VIIa (FVIIa) polypeptides, where said variants comprise an amino acid substitution in position 10 and 32 and where said variants further comprise a sugar moiety covalently attached to an introduced in vivo N-glycosylation site located outside the Gla domain. Such polypeptide variants are useful in therapy, in particular for the treatment of a variety of coagulation-related disorders, such as trauma.

The present invention relates to novel polypeptide variants of factor VII (FVII) or factor VIIa (FVIIa) polypeptides, where said variants comprise an amino acid substitution in position 10 and 32 and where said variants further comprise a sugar moiety covalently attached to an introduced in vivo N-glycosylation site located outside of the Gla domain. Such polypeptide variants are useful in therapy, in particular for the treatment of a variety of coagulation-related disorders, such as trauma.

The invention relates to a method for selective enrichment and identification of N-glycosylation sites in a biological sample based on a reductive amination reaction of amino and aldehyde groups and application of the method to proteomic analysis of N-glycoprotein. The method comprises the following steps: taking a biological sample containing glycoprotein, carrying out enzymatic hydrolysis by using trypsin and then blocking the terminated amino group and side-chain amino group of a peptide fragment by using formaldehyde; then oxidizing a sugar chain on glycopeptide by using a sodium periodate solution so as to produce an aldehyde group; then enriching oxidized glycopeptide with amino microspheres and carrying out treatment with peptide glycosidase PNGase F; and carrying out mass spectrometry on released N-glycopeptide so as to obtain proteome information of N-glycoprotein in the sample. The method can be used for proteomic analysis of glycosylation and can acquire identification results of corresponding glycoprotein, glycopeptide and glycosylation sites at the same time. The method is simple and highly efficient and has high throughput in identification of N-glycosylation sites on glycoprotein.

The present invention relates to novel conjugates between polypeptide variants of protein C and a non-polypeptide moiety, such as PEG or sugar moieties. In particular, the present invention provides novel protein C conjugates having an increased resistance to inactivation by e.g. human plasma and α1-antitrypsin. Consequently, such conjugates have an increased in vivo half-life. Preferred examples include protein C conjugates, wherein at least one additional in vivo N-glycosylation site has been introduced. The conjugates of the invention are useful for treating a variety of diseases, including septic shock.

The invention discloses a method for increasing lipase expression through glycosylation modification as well as a mutant enzyme and an application thereof and belongs to the field of enzyme engineering. The N-glycosylation mutation is performed on a leading peptide sequence of rhizopus oryzae lipase, the SAS and / or NT amino acid are / is respectively modified to an N-glycosylation site NGT and / or NLT, the extracellular protein concentrations of the obtained mutant enzyme proROLA, proROLB and proROLAB are increased by 211%, 188% and 233% compared with those of the un-glycosylated proROL, the enzyme activities when culturing in a fermentation tank are respectively 8210 U.mL<-1>, 8457 U.ml<-1> and 9366 U.mL<-1>, and the un-mutated proROL extracellular enzyme activity is almost zero. The rhizopus oryzae lipase provided by the invention has obviously increased lipase enzyme activity and can be used in the fields such as food, chemical engineering and biological energy source.

The present invention relates to novel interferon gamma polypeptide variants having interferon gamma (IFNG) activity, methods for their preparation, pharmaceutical compositions comprising the polypeptide variants and their use in the treatment of diseases, in particular for the treatment of interstitial pulmonary diseases, such as idiopathic pulmonary fibrosis. These novel polypeptide variants all comprise the substitution S99T as compared to the amino acid sequence of huIFNG or fragments thereof. By performing this mutation the naturally occurring N-glycosylation site present at position 97 is significantly better utilized. Preferably, the variants comprise further modifications, e.g. in order to increase the AUC of such variants when administered subcutaneously.

The present invention relates to modified HIV-1 envelope proteins where one or more N-glycosylation sites have been deleted or modified, which produce a broadly cross reactive neutralizing response, their methods of use and antibodies which bind to these proteins. The invention also provides for nucleic acids, vectors, antibodies and pharmaceutical compositions that comprise said modified HIV-1 envelope proteins.

The present invention relates to novel conjugates between polypeptide variants of protein C and a non-polypeptide moiety, such as PEG or sugar moieties. In particular, the present invention provides novel protein C conjugates having an increased resistance to inactivation by e.g. human plasma and alpha1-antitrypsin. Consequently, such conjugates have an increased in vivo half-life. Preferred examples include protein C conjugates, wherein at least one additional in vivo N-glycosylation site has been introduced. The conjugates of the invention are useful for treating a variety of diseases, including septic shock.

The invention relates a gene for coding low-temperature alkaline lipase (LipY). The gene is obtained by cloning cold-adapted marine yeast (BoHai Sea-9145), which can secrete low-temperature alkaline lipase and is screened from sea mud of Bohai, by a method of combining degenerate primer polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE). The gene contains a 1206bp open reading frame (ORF), codes 401 amino acids and contains a signal peptide with 28 amino acids at the amino acid end. Each coded amino acid contains a conserved sequence (G-X-S-X-G) of the lipase and a potential N-glycosylation site. The lipase gene is cloned on an eukaryotic expression vector (pIc9K) and performs heterologous expression in Pichia pastoris (Gs115). High-activity recombinant lipase is obtained from supernate 96 hours after fermentation, wherein methanol serves as an inducer. An electrophoretic pure lipase sample is obtained by purifying the recombinant lipase by a nickel ion affinity column. The recombinant lipase has a series of advantages of high low-temperature catalytic activity, stable performance under the alkaline condition and the like, and has wide application prospect in the low-temperature catalytic fields of daily chemicals, textile, food processing and the like.

The invention discloses a N glycosyl transferase AaNGT. An amino acid sequence thereof is shown as SEQ ID NO.1. The invention also discloses an application of the N glycosyl transferase AaNGT in polypeptide galactosylated modification. AaNGT is capable of supplying a simple and convenient method for the forming of glycopeptide; the capacity of AaNGT for utilizing UDP-Glc is obviously higher than that of the reported N glycosyl transferase ApNGT which is sourced from Actinobacillus pleuropneumoniae; the AaNGT also can transfer the special glycosyl donor UDP-GlcNH2 to the polypeptide containing N glycosylation sites, and then the other glycosyl transferases can be utilized to convert the GlcNH2 into the GlcNAc so as to form natural N glycan linking, so that a new method is supplied for the development of the glycoprotein vaccine. The invention is beneficial to the AaNGT becoming a protein modified tool enzyme.

The present application in one aspect provide Fc-containing polypeptide conjugates comprising an Fc-containing polypeptide conjugated to a conjugate moiety, wherein the Fc-containing polypeptide comprises an N-glycosylated Fc region comprising an acceptor glutamine residue flanked by an N-glycosylation site and wherein the conjugate moiety is conjugated to the Fc-containing polypeptide via the acceptor glutamine residue. Also provided are methods of making such Fc-containing polypeptide conjugates by using a wildtype or engineered transglutaminases. Further provided are engineered transglutaminases specifically designed for carrying out such reactions.

The invention discloses low-saccharification mutant interferon lambda1 as well as expression and purification methods thereof. The low-saccharification mutant interferon lambda1 is protein obtained after replacing Asn in the N46 bit in the IFN-lambda1 mature peptide sequence into Gln. The blockage on the high-mannose modification process of the N-glycosylation site of the IFN-lambda1 by the Pichia pastoris is realized so that the IFN-lambda1-Nm genes are correctly expressed in the Pichia pastoris in a high-efficiency secretory way, the uniformity of the expressed recombination IFN-lambda1-Nm protein is obviously improved, the expression level is higher than 65mg / L, the purity of the expressed recombination protein after two-step column chromatography is higher than 98 percent, and the low-saccharification modified recombination IFN-lambda1-Nm protein has the same biological activity as the original Pichia pastoris expressed wild IFN-lambda1 and simultaneously has better safety. The invention provides an effective method for efficient low-cost mass production of low-saccharification mutant interferon lambda1 with biological activity and good uniformity, higher practical application values are realized, and the application prospects are wide.

The present application in one aspect provide Fc-containing polypeptide conjugates comprising an Fc-containing polypeptide conjugated to a conjugate moiety, wherein the Fc-containing polypeptide comprises an N-glycosylated Fc region comprising an acceptor glutamine residue flanked by an N-glycosylation site and wherein the conjugate moiety is conjugated to the Fc-containing polypeptide via the acceptor glutamine residue. Also provided are methods of making such Fc-containing polypeptide conjugates by using a wildtype or engineered transglutaminases. Further provided are engineered transglutaminases specifically designed for carrying out such reactions.

The invention belongs to the field of a therapeutic biological preparation, and particularly relates to new carboxyl terminal peptide. The invention further relates to an application of the new carboxyl terminal peptide and long-acting interferon made from the carboxyl terminal peptide. Through a synergistic effect of an O-glycosylation site and an N-glycosylation site, the half life of a protein molecule fused with the carboxyl terminal peptide is prolonged, and the activity of the fused protein molecule isn't obviously affected or is enhanced.

The present invention relates to novel conjugates between polypeptide variants of protein C and a non-polypeptide moiety, such as PEG or sugar moieties. In particular, the present invention provides novel protein C conjugates having an increased resistance to inactivation by e.g. human plasma and alpha1-antitrypsin. Consequently, such conjugates have an increased in vivo half-life. Preferred examples include protein C conjugates, wherein at least one additional in vivo N-glycosylation site has been introduced. The conjugates of the invention are useful for treating a variety of diseases, including septic shock.

The invention provides a full-length cDNA of the wingless gene of Periplaneta americana and an encoded protein and cloning method thereof, and application thereof. The full-length cDNA of the wingless gene of Periplaneta americana is named as PaWg, with an accession number of KJ680328 in the GeneBank. The full-length sequence of the cDNA has a length of 1, 456 bp; the open reading frame (ORF) of the cDNA contains 1, 194 bases, coding 397 amino acids; and the nucleotide sequence of the cDNA is as shown in SEQ ID No. 1 in a sequence table. The amino acid sequence of the protein coded by the gene sequence is as shown in SEQ ID No. 2 in the sequence table. The protein has a theoretical isoelectric point PI of 9.46 and a molecular weight of 4.4 kD and is a stable hydrophilic protein. The segment, composed of amino acids from site 1 to site 21, of the amino acid sequence of the protein is a segment of signal peptide; an enzyme site is located between site 21 and site 22; and the protein may contain two N-glycosylation sites, respectively located on asparagine at site 107 and site 343. The invention provides application of the gene, the protein and the cloning method to preparation of cockroach pesticides and biological probes and lays a foundation for research and application related to the wingless gene of Periplaneta americana.

The present application in one aspect provide Fc-containing polypeptide conjugates comprising an Fc-containing polypeptide conjugated to a conjugate moiety, wherein the Fc-containing polypeptide comprises an N-glycosylated Fc region comprising an acceptor glutamine residue flanked by an N-glycosylation site and wherein the conjugate moiety is conjugated to the Fc-containing polypeptide via the acceptor glutamine residue. Also provided are methods of making such Fc-containing polypeptide conjugates by using a wildtype or engineered transglutaminases. Further provided are engineered transglutaminases specifically designed for carrying out such reactions.

There is provided a DNA molecule derived from a prokaryotic cell in which at lest one of the DNA regions encoding NXB (N is asparagine, X is any amino acid other than proline, and b is serine or threonine) has been modified so that no N-glycosylation occurs buring the expression in a eukaryotic cell, and since the DNA molecule has been modified at the N-glycosylation site, it produces a non-N-glycosylated protein, which thereby exhibits a high immunogenicity when, for example, it is allowed to produce, in a eukaryotic cell, an antigen protein derived from a prokaryotic cell.

The present invention concerns a recombinant or transgenic factor VII compound, each factor VII molecule of the compound having glycan forms linked to N-glycosylation sites, wherein among all the factor VII molecules in said compound, glycan, biantennary, bisialylated and non-fucosylated forms are in the majority. The invention also concerns such a compound for use as a medication, and a method for preparing said compound, among others.

The invention provides a method of improving enzymatic thermostability via artificially designed glycosylation modification and belongs to the field of bioengineering. The method includes: subjecting a primary sequence of enzyme protein and its spatial structure to combinatory analysis, designing an N-glycosylation site on a subunit joint of the enzyme protein or on a joint of internal motifs of subunits of the enzyme protein, and utilizing site-directed mutagenesis to introduce an N-glycosylation modification feature recognition enhancer sequon so as to enable carbohydrate chains to form a specific glycosyl hairpin structure among subunits of the enzyme protein or form a specific glycosyl pad structure among the internal motifs of the subunits of the enzyme protein, thereby maximally improving the rigidity of the tertiary structure of the enzyme protein and stabilizing the spatial conformation of the enzyme protein to be free from stress of high-temperature environments. The method of enzyme protein modification has the advantages that thermostability of the enzyme protein is greatly improved, the improvement in the thermostability of the enzyme protein is under artificial control, enzyme activity is partly improved, and catalytic features of the enzyme protein are enhanced.

The invention discloses a N-glycosylation algin lyase mutant capable of being efficiently applied and a genetic engineering bacterium construction method, and belongs to the technical field of biology. Based on algin lyase Aly-Cob, a DQNAT repeated sequence fragment is introduced at an N- terminal to be used as an N-glycosylation site, besides, expression carrier protein YebF is fused to be subjected to exocytosis, and further a glycosylation system pgl from campylobacter jejuni performs glycosylation modification through a sugar chain GalNAc2(Glc)GalNAc3Bac. The enzyme activity and the stability of an obtained mutant enzyme NACob are improved than those of an enzyme before glycosylation. The N-glycosylation modified algin lyase mutant provided by the invention can be widely applied to thefields of foods, medicines, agriculture and the like relevant to seaweed processing and utilizing.

The invention discloses an SARS-CoV-2 receptor bind region (RBD) glycosylation modification antigen, which is characterized in that the N glycosylation site of the SARS-CoV-2 receptor bind region (RBD) is truncated and connected in series to form a single-chain polymer form, and natural glycosyl shielding on the surface of the RBD is reduced so as to more favorably expose antigen epitopes. Compared with a wild RBD monomer and RBD polymer form, the antigen can excite an organism to generate a higher-level SARS-CoV-2 specific antibody and a neutralizing antibody; an immune effect obtained through one-needle immunization can be equal to the immune effect of two-needle immunization of the wild type RBD monomer; and immune serum has similar neutralizing titer by aiming at an original strain and a variant strain, and shows a good cross protection effect. The invention also provides application of the glycosylation modification antigen in preparation of SARS-CoV-2 treatment and prevention drugs or vaccines.

The invention concerns a recombinant or transgenic factor VII composition, each factor VII molecule of the composition having two N-glycosylation sites, wherein among all the FVII molecules of the composition the proportion of glycan units Gala1, 3Gal ranges between 0 and 40%. The invention also concerns a method for preparing such a FVII composition.