44 results about "Macromolecular binding" patented technology

The present invention relates, in part, to methods and compositions for needleless delivery of macromolecules to a subject. In one aspect, the methods and compositions involve administering to the subject a delivery construct comprising a carrier construct non-covalently bound to a binding partner, wherein the carrier construct comprises a receptor-binding domain, a transcytosis domain, and a macromolecule to which the binding partner non-covalently binds, wherein the binding partner binds to the macromolecule with a K_a that is at least about 10⁴ M⁻¹.

Disclosed are methods for detecting and quantitatively measuring a binding property of a compound to a target macromolecule, wherein the target macromolecule is subject to denaturation and is linked to a labeling peptide, such as a short enzyme fragment. The method uses a fluid mixture comprising (i) a chimeric molecule comprising a target macromolecule linked to the labeling peptide and (ii) a compound being measured for binding to the target macromolecule, wherein said target macromolecule is subject to denaturation. After allowing for binding of the compound (e.g. a small molecule inhibitor of the target macromolecule), one detects a signal from the labeling peptide, such as by enzyme fragment complementation. This signal indicates a differential between denatured and non-denatured target macromolecules and thereby indicates a differential between target macromolecules not bound to the compound and target macromolecules bound to the compound, respectively.

This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.

The invention is manufactured for aiding in removing moisture, smell and bacterial when people wear masks, in the meantime, is able to release negative ions and infrared ray that are favorable for human body, and provides a formulation of raw materials for an antibiotic, moisture-absorbing, smell-eliminating and healthcare mask and a production process thereof, the formulation of raw materials thereof includes: macromolecular binding agent, bamboo charcoal powder, surfactant and polypropylene fiber film. The proportion in the formulation of raw materials of the mask is as below: 10-50% of macromolecular binding agent, 0.1-30% of bamboo charcoal powder, 0.1-20% of surfactant and 1-10% of polypropylene fiber film. The process flow of the mask includes the steps of: adding a polypropylene fiber film interlayer into a filtration layer of the mask; then binding the bamboo charcoal powders using the macromolecular binding agent to form a bamboo charcoal powder layer as a surface layer of the mask, afterwards, adding the surfactant on the surface layer thereof; eventually, combining the layers of the mask together by a mechanically sewing process.

The invention discloses a method for preparing TiO2@Au core-shell structure in a template method. The method comprises the following steps: preparing PS@Au in a template method, coating the PS@Au witha layer of amorphous titanium dioxide to obtain TiO2@PS@Au, and calcining the TiO2@PS@Au to obtain TiO2@Au. PS is used as a template, Au is wrapped in the TiO2, a uniform and stable TiO2@Au core-shell structure can be obtained after the calcinations, and the dispersion property is good. The core-shell structure is a hollow structure, the weight is light, and the surface area is relatively large under the same mass. Nano gold can be combined with various biological macromolecules, the bioactivity is not influenced, the nano gold can be applied to the biological catalysis and biological sensing, the TiO2@Au obtained by the invention can be used as an enzyme reactor, compared with the method only adopting the pure titanium dioxide as the carrier, the separation of the nano gold in the subsequent reaction can be avoided, the advantages of the titanium dioxide semiconductor can be played, and the overall catalytic efficiency can be increased.

The present invention relates to a novel tobacco fermenting enzyme preparation production method. The purpose is to solve the technical problems that how improve the quality of the tobacco fermenting product is improved and how the reactivity protection of the tobacco fermenting enzyme preparation is realized in the natural fermenting field of tobacco. The enzyme preparation consists of a glucoseoxidase, a chlorophyl oxidase, a carotenoid oxidase, a protease, and a nicotine-degradation enzyme. Through the cell disruption of fresh leaves, (NH ₄) ₂ SO ₄ is utilized to operate the second fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca ^{2 +} and Mg ^{2 +} to operate the metal ion exchange, to accomplish the molecule modification; a macro molecule combination modification is accomplished through adopting 0.01 percent of cane sugar low molecular polymer, thereby prolonging the half life period of theenzyme preparation and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the nicotine is decreased by 9.3 percent, the total nitrogen is decreased by 5.7 percent, the protein is decreased by 7.1 percent; cigarette smoke condensates are decreased by 8.4 percent, the tar content is decreased by 5.1 percent, the cigarette smoke nicotine content is decreased by 28.0 percent, and the carbon monoxide is decreased by 1.6 percent. The enzyme preparation is employed when the tobacco leaf is wet for the second time before defolat and redrying.

The present invention provides a preparation method and related products of SrVO3 nanofibers. The method comprises the steps of dissolving a strontium-containing soluble compound and a vanadium-containing soluble compound in a solvent, adding a macromolecular binding agent, and stirring the components uniformly to obtain an electrostatic spinning solution; transferring the electrostatic spinning solution into an electrostatic spinning device for electrostatic spinning to obtain precursor nanofibers and; putting the precursor nanofibers into air for peroxidization calcining to obtain SrO/V2O5 nanofibers, and then performing calcining in a reducing atmosphere to obtain the SrVO3 nanofibers of one-dimensional structures. The application structures of traditional block bodies and powder are changed.

The invention provides an ethoxylated trimethylolpropane core hyperbranched polymer taking dithiocarboxylate as a side group and an end group and a preparation method and application of the ethoxylated trimethylolpropane core hyperbranched polymer as a heavy metal chelating agent, and relates to the technical field of chemical engineering and environmental protection. The chemical formula of the ethoxylated trimethylolpropane core hyperbranched polymer taking dithiocarboxylate as a side group and an end group is CH3CH2C [CH2OCH2CH2OCOCH2CH2CH2N (CSSM) CH2CH2NHCSSM] 3, wherein M is Na<+>, K<+>or NH4<+>. The hyperbranched polymer provided by the invention is simple in preparation method, easily available in raw materials and easy to industrialize. The hyperbranched polymer can be used as aheavy metal chelating agent, the special three-dimensional space structure of the hyperbranched polymer can be alternately chelated with heavy metals to form a three-dimensional super-macromolecular combination with low solubility and strong stability, and wastewater and wastes containing heavy metals can be effectively treated.