The invention discloses a bitterness-free
shrimp-type low-molecular-weight peptides as well as a preparation method and application thereof, belonging to the technical field of
biology. The invention provides
bacillus subtilis YBPE-4 with a preservation number of CCTCC NO: M2016359. The preparation method comprises the following steps:
drying and crushing shrimps, enzymolyzing the shrimps by using
neutral protease, and separating to obtain an enzymolysis solution; preparing a
fermentation culture medium by utilizing the enzymolysis solution, soluble
starch and
potassium dihydrogen
phosphate, inoculating a mature bacillus subtiliss YBPE-4 culture solution, and stirring, ventilating and fermenting for 132 to 144 hours; centrifuging a fermented solution to remove
thallus to obtain supernatant, and filtering by virtue of an
ultrafiltration membrane to obtain filtrate with a molecular weight of 360 to 5000 Da; and
drying the filtrate to finally obtain the bitterness-free
shrimp-type low-molecular-weight
peptide product. The bitterness-free
shrimp-type low-molecular-weight peptides have the advantages that the bitterness removal effect is good and the yield of products is high, and the preparation method is relatively low in production cost, suitable for producing the bitterness-free low-molecular-weight peptides by utilizing low-value shrimps in an industrialization manner and good in application prospect.