43 results about "Lonicera hypoglauca" patented technology

The invention discloses a method for distinguishing lonicera hypoglauca miq and honeysuckle, which distinguishes the lonicera hypoglauca miq and the honeysuckle by utilizing thin-layer chromatography. The method further distinguishes the lonicera hypoglauca miq and the honeysuckle by utilizing high performance liquid chromatography. The distinguishing method can be used for evaluating real or fake lonicera hypoglauca miq or honeysuckle and the quality of both, used for distinguishing lonicera hypoglauca miq medicine material and honeysuckle medicine material, and also can be used for quality evaluation of feeding medicine materials in preparations so as to ensure the stable, uniform and controllable quality of the preparations. The method has the advantages of simple and convenient operation, good repeatability, strong visual impression and the like.

The invention discloses a method for preparing chlorogenic acid from leaves of lonicera hypoglauca miq. The method comprises the following steps of: carrying out pretreatment on medicinal materials; carrying out reflux extraction on ethanol; concentrating; filtering; carrying out flocculation and sedimentation; concentrating; obtaining a crude product of chlorogenic acid; carrying out recrystallization; and obtaining a fine product of chlorogenic acid. The method obtains the optimal process conditions of separating from traditional Chinese leaves of lonicera hypoglauca miq and purifying the chlorogenic acid, i.e. the optimal conditions are screened for a process of separating and purifying the chlorogenic acid in the leaves of lonicera hypoglauca miq by single factors and an orthogonal test, an orthogonal table L9(34) is adopted and testing is carried out by using the ethanol volume, the ethanol concentration, pH and flow rate and using the maximum ultraviolet adsorption wavelength of 320nm of the chlorogenic acid as an index. The obtained optimal elution process conditions of separating and purifying the chlorogenic acid from macroporous resin in the leaves of lonicera hypoglauca miq are that the ethanol volume is 20ml, the ethanol concentration is 20 percent, the flow rate is 80 drops per minute, the pH is 7 and extraction efficiency is 1.43 percent; and the optimal feeding conditions are that the solid-to-liquid ratio is 1g:6ml, the pH of ethanol is 2 and the flow rate is 40 drops per minute.

The invention discloses triploid lonicera confusa and a cultivation method thereof. The cultivation method comprises the following steps: taking grown-up plants of diploid lonicera hypoglauca miq and lonicera macranthoides as original parents, planting the parents according to the conventional technology, and then mutually pollinating corresponding plants in a hybridization manner; inducing the parents to form diploid pollen when the parents just enter a green bud period; picking the induced flower in a white bud period, taking out and drying anther, and then screening the diploid pollen for low temperature storage; hybridizing the diploid pollen with haploid oocytes of corresponding plants to obtain hybrid seeds; breeding the hybrid seeds into complete plants and then screening triploid plants; and planting the triploid plant according to the conventional technology, and budding to obtain triploid lonicera confusa. Compared with the prior art, by adopting the method, the diploid pollen can be screened more quickly, the difficulty of sterility in a hybridization period can be solved, the breeding time can be obviously shortened, the operation is simple and feasible, the genetic advantages of the triploid lonicera confusa are higher than those of diploid or tetraploid plants, and the planting economic benefit is high.

The present invention relates to lonicera hypoglauca miq effective region extract, namely, stem and leaf extract of lonicera hypoglauca miq. Its preparation method includes the following steps: using lonicera hypoglauca miq, stem and leaf as raw material, degreasing by using low-polarity organic solvent, using ethyl alcohol to make extraction to obtain ethyl alcohol extract, concentrating, feeding the concentrate into macroporous resin column, using water to make elution, then using ethyl alcohol to make elution, collecting ethyl alcohol eluent, concentrating eluent, centrifugalization to remove precipitate, taking out supernatant fluid, using n-butyl alcohol to make extraction, concentrating extract and drying so as to obtain the invented product with high activity for protecting liver.

The invention belongs to the field of health food, and particularly relates to a greengage-containing auxiliary blood glucose lowering health food composition and a preparation method thereof. The greengage-containing auxiliary blood glucose lowering health food composition comprises the following preparation raw materials by weight: 30-40 parts of greengage, 10-20 parts of passiflora edulia sims, 5-15 parts of intermediate peashrub flower, 5-15 parts of lychee shell, 8-15 parts of lonicera hypoglauca, 10-15 parts of avena chinensis, 8-18 parts of bamboo leaf, 6-13 parts of L-arabinose, 3-7 parts of manuka honey freezing drying powder, 5-10 parts of peach gum polysaccharide, 0.2-0.4 part of sodium hexametaphosphate, and 0.1-0.2 part of sodium D-isoascorbate. According to the present invention, the greengage-containing auxiliary blood glucose lowering health food composition has advantages of scientific and reasonable compatibility, no preservative and essence, safety, stability, low toxic-side effect, synergistic action of various components, significant auxiliary blood glucose lowering effect, strong stability, and easy long-term preservation achieving.

The invention discloses an adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca. The method comprises the steps of carrying out adventitious bud induction on the petioles of the sterile tissue culture seedlings of Lonicera hypoglauca with the seedling age of 45-55 days, carrying out wound area increasing treatment on the petioles, then, inoculating the treated petioles to an adventitious bud induction culture medium for culture so as to induce petiole callus tissue, and continuing to culture the petiole callus tissue so as to induce adventitious buds, wherein conditions for both callus tissue induction culture and adventitious bud induction culture are as follows: the culture temperature is 25+ / -3 DEG C, the relative humidity is 50-70%, the illumination is 12 hours per day, and the intensity of illumination is 2,000-3,000Lux. The method disclosed by the invention is simple and convenient, and the adventitious buds can be rapidly and effectively induced from the Lonicera hypoglauca petioles in a short time, so that new technical reference is provided for the excellent germplasm preservation and rapid breeding of Lonicera hypoglauca and application of Lonicera hypoglauca in research and production.