Adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca

Abstract

The invention discloses an adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca. The method comprises the steps of carrying out adventitious bud induction on the petioles of the sterile tissue culture seedlings of Lonicera hypoglauca with the seedling age of 45-55 days, carrying out wound area increasing treatment on the petioles, then, inoculating the treated petioles to an adventitious bud induction culture medium for culture so as to induce petiole callus tissue, and continuing to culture the petiole callus tissue so as to induce adventitious buds, wherein conditions for both callus tissue induction culture and adventitious bud induction culture are as follows: the culture temperature is 25+ / -3 DEG C, the relative humidity is 50-70%, the illumination is 12 hours per day, and the intensity of illumination is 2,000-3,000Lux. The method disclosed by the invention is simple and convenient, and the adventitious buds can be rapidly and effectively induced from the Lonicera hypoglauca petioles in a short time, so that new technical reference is provided for the excellent germplasm preservation and rapid breeding of Lonicera hypoglauca and application of Lonicera hypoglauca in research and production.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for inducing petiole adventitious buds of aseptic tissue culture seedlings of Lonicera red glandularis. Background technique [0002] Red gland honeysuckle (Lonicerahypoglauca Miq.), also known as pan gland honeysuckle, wild gland honeysuckle, gland back honeysuckle, etc., is a plant of Lonicera (Lonicera), a perennial semi-entangled vine or rattan shrub, with hollow old stems, mainly produced in Henan, Guangxi, Shandong, Yunnan and other places. The 2005 edition of "Chinese Pharmacopoeia" stipulated Lonicera hypoglauca Miq., Lonicera nuwranthoides Hand. Mazz. and Lonicera confuse DC. as three sources of Shanyinhua (one of the precious Chinese medicinal materials in my country). There is a large social demand for Lonicera red glandularis, and the gap between production and demand is large. Its medicinal value and health care function are enough to promote the develop...

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Problems solved by technology

At present, there are few reports on the tissue culture of Lonicerae japonicus, and there are not many reports on the tissue culture of Lonicera red glandularis. Lonicera red glandularis belongs to woody vines, and tissue culture is difficult. At present, conventional seedlings ( The stems with axillary buds or terminal buds of conventional seedlings grown in non-sterile environments such as mountains or experimental bases are used as explants for tissue culture, and the semi-lignified stems with axillary buds are the preferred material, which need to be sterilized After sterilization, the first-generation buds are induced to solve some technical problems within the range. However, as the first-generation buds continue to be cultivated, some problems will appear later, such as the performance of endophytes, which make the final aseptic seedlings grow into seedlings. The rate is extremely low, which hinders the progress of transplanting strong seedlings. The cost of seedling cultivation is high, and it is not used in practice on a large scale. However, there is no report on the induction of adventitious buds on petioles of Lonicera red glandular tissue culture seedlings. The present invention has been passed by the applicant through a large number of After exploring and experimenting, a new method of inducing aseptic seedlings of Lonicera red glandularis was found, that is, the petiole of Lonicera red glandularis was used as the induction object, and a complete aseptic seedling plant was successfully induced, which provided a new method for basic research and applied research in this field. the reference direction

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