Method for preparing chlorogenic acid from leaves of lonicera hypoglauca miq

Abstract

The invention discloses a method for preparing chlorogenic acid from leaves of lonicera hypoglauca miq. The method comprises the following steps of: carrying out pretreatment on medicinal materials; carrying out reflux extraction on ethanol; concentrating; filtering; carrying out flocculation and sedimentation; concentrating; obtaining a crude product of chlorogenic acid; carrying out recrystallization; and obtaining a fine product of chlorogenic acid. The method obtains the optimal process conditions of separating from traditional Chinese leaves of lonicera hypoglauca miq and purifying the chlorogenic acid, i.e. the optimal conditions are screened for a process of separating and purifying the chlorogenic acid in the leaves of lonicera hypoglauca miq by single factors and an orthogonal test, an orthogonal table L9(34) is adopted and testing is carried out by using the ethanol volume, the ethanol concentration, pH and flow rate and using the maximum ultraviolet adsorption wavelength of 320nm of the chlorogenic acid as an index. The obtained optimal elution process conditions of separating and purifying the chlorogenic acid from macroporous resin in the leaves of lonicera hypoglauca miq are that the ethanol volume is 20ml, the ethanol concentration is 20 percent, the flow rate is 80 drops per minute, the pH is 7 and extraction efficiency is 1.43 percent; and the optimal feeding conditions are that the solid-to-liquid ratio is 1g:6ml, the pH of ethanol is 2 and the flow rate is 40 drops per minute.

Description

technical field [0001] The invention belongs to the technical field of chlorogenic acid preparation, in particular to a method for preparing chlorogenic acid from honeysuckle leaves. Background technique [0002] Red Gland Honeysuckle [1，2] It is the dry flower bud or the flower with the first bloom of Lonicera hypoglauca Miq., a plant of Lonicera family Lonicera hypoglauca Miq. Guangxi and other places are one of the main sources of Shanyinhua medicinal products. Honeysuckle is a commonly used clinical traditional Chinese medicine. It is sweet in taste and cold in nature. It is used to treat carbuncle, sore throat, erysipelas, bloody dysentery, wind-heat cold, and fever. It is an important medicine for clearing away heat and detoxifying. It has inhibitory effect on a variety of pathogenic bacteria and is a broad-spectrum antibacterial traditional Chinese medicine, known as "penicillin in traditional Chinese medicine". Chemical and pharmacological studies confirm [3，4] I...

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Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a method for preparing chlorogenic acid from Lonicera red glandular leaves, aiming at solving the problem of extracting chlorogenic acid from honeysuckle as an industrial raw material for a long time, but the price is expensive, and the development and utilization of other plants of the same genus are not enough, especially It is a problem of insufficient attention and research on the leaves of red glandular honeysuckle

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