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Method for identifying lonicera hypoglauca with different bases

A technology of syringae and base, which is applied in the field of chemical medicine and traditional Chinese medicine ingredient research, can solve the problems of inability to distinguish syringae, and the applicability of identification personnel is not strong, and achieves good reproducibility, strong characteristics, and map information. rich effects

Pending Publication Date: 2022-07-01
GUANGDONG YIFANG PHARMA +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] There are slight differences in the native plants, medicinal materials (decoction pieces) and microscopic characteristics of the three different bases of Shanyinhua, but the overall shape is very similar, and the applicability is not strong for those with inexperienced identification
Especially for the Jasmine Japonica extract and related preparations when the original plant traits cannot be obtained or the medicinal materials, the shape of the decoction pieces, and the microstructure are lost, the traditional identification methods cannot effectively distinguish the Jasmine Jasmine from different bases

Method used

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  • Method for identifying lonicera hypoglauca with different bases
  • Method for identifying lonicera hypoglauca with different bases
  • Method for identifying lonicera hypoglauca with different bases

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Embodiment 1

[0077] The present embodiment provides a kind of identification method of the different bases of silver flower, which is specifically as follows:

[0078] 1. Instruments and Reagents

[0079] 1.1 Instruments

[0080] Thermo ultra-high performance liquid chromatograph (Vanquish, Thermo Fisher Scientific (China) Co., Ltd.), WatersACQUITY HSS T3 column (100mm×2.1mm, 1.8μm), 1 / 10,000 analytical electronic balance (ME204E, Mettel) Le-Toledo Corporation), one-millionth analytical electronic balance (XP26, METTLER TOLEDO Corporation), ultrapure water system (Milli-QDirect, Merck AG).

[0081] 1.2 Reagents

[0082] Methanol (Xilong Science Co., Ltd.) analytically pure; Phosphoric acid (Tianjin Kemeiou Chemical Reagent Co., Ltd.), acetonitrile (Merck Co., Ltd.) HPLC chromatographic grade for liquid phase, ultrapure water (laboratory self-made) .

[0083] 1.3 Reagents

[0084] Chlorogenic acid (batch number: 110753-201817, content: 96.80%, China National Institute for Food and Drug...

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Abstract

The invention provides a method for identifying lonicera confusa with different base sources, which comprises the following steps: taking neochlorogenic acid, chlorogenic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-O-dicaffeoylquinic acid, lonicera macranthoides saponin B, dipsacus asper saponin B, cryptochlorogenic acid, isochlorogenic acid B, secoxyloganin and lonicera macranthoides saponin A reference substances; preparing a mixed reference substance solution; the method comprises the following steps: respectively preparing test sample solutions of different original lonicerae flos from samples of different original lonicerae flos; respectively carrying out ultra-high performance liquid chromatography analysis on the mixed reference substance solution and the test solutions of the different original lonicerae flos, and respectively constructing characteristic spectrums of the different original lonicerae flos; and taking an object to be detected, carrying out ultra-high performance liquid chromatography analysis, and comparing the obtained spectrum of the object to be detected with the characteristic spectrum of the lonicera confusa with different bases. The identification method has the advantages of being simple to operate, rich in map information, strong in characteristic and good in reproducibility.

Description

technical field [0001] The invention relates to the technical field of research on chemical medicines and traditional Chinese medicine ingredients, in particular to a method for identifying the different bases of silver flowers. Background technique [0002] The 2020 edition of the Pharmacopoeia of the People's Republic of China stipulates that the base of the honeysuckle is Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera confusa DC. or Lonicera Lonicera Dried flower buds or flowers with first blooms of fulvotomentosa Hsu et S.C.Cheng. The identification of the origin of traditional Chinese medicine is the main basis for the inheritance, research, production, development and utilization of traditional Chinese medicine. There are many types and sources of Chinese medicinal materials, and the correct medicinal material base is an important prerequisite to ensure accurate medication and quality control. At present, the identification methods of differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N2030/065
Inventor 朱德全丁青刘晓霞李振雨孙冬梅陈向东陶晨璐
Owner GUANGDONG YIFANG PHARMA
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