299 results about "Immunologic Technique" patented technology

The present invention provides novel osmium-based electrochemical species for the detection of wide variety of analytes using immunological techniques. The present invention also provides diagnostic kits and test sensors supporting electrode structures that can be used with the osmium-based electrochemical species. The test sensor can be fabricated to support interdigitated arrays of electrodes that have been designed to provide amplification of the electrical signal amplification desired to analyze analytes that may be present at low concentrations.

The invention belongs to the field of immunological technique and discloses a method for lymphocyte subsets and a kit thereof. The method of lymphocyte subsets provided by the invention includes T lymphocyte subsets and B lymphocyte subsets. T lymphocyte subsets contain the following steps of: getting different fluorescence labeled antibodies, mixing with a sample to be detected, incubating, detecting by flow cytometry to obtain test data and analyzing the test data. B lymphocyte subsets contain the following steps of: getting different fluorescence labeled antibodies, mixing with a new aforementioned sample to be detected, incubating, detecting by flow cytometry to obtain test data and analyzing the test data. The method provided by the invention is used to realize a more comprehensive fine immunophenotyping of lymphocyte, has advantages of less sample needed to be detected, simple operation, short time needed and high precision, and can be widely applied in immunophenotyping of lymphocyte subpopulation.

The invention discloses a tumor marker CD25 autoantibody and application of the tumor marker CD25 autoantibody, belonging to the technical field of immunology. The invention provides an amino acid sequence of antigen of immune response regulation gene CD25. The CD25 polypeptide antigen is used for detecting corresponding specific autoantibody in blood of lung cancer and esophageal cancer patients; and the autoantibody can be used as a tumor marker to evaluate risk level of occurrence of lung cancer and esophageal cancer. The antigen polypeptide and the antibody can be used for preparing early-stage tumor diagnostic reagent and developing target drugs for treating tumor.

The invention relates to a colloidal gold sensitization chromatography test strip for quickly detecting copper ions, which belongs to the technical field of immunology, and comprises a lining board, a sample cushion, a gold-marking combined cushion, a sensitization cushion, a coating film and a water absorption cushion, wherein the sample cushion, the gold-marking combined cushion, the sensitization cushion, the coating film and the water absorption cushion are sequentially jointed on the lining board; the gold-marking combined cushion is made of glass fiber cotton and used for absorbing small gold mark copper ion antibodies; the sensitization cushion is made of glass fiber cotton and used for absorbing large gold mark goat anti-mouse IgG (immunoglobulin G); and the coating film is provided with a linear invisible detection line T and a linear invisible control line C which are arrayed in parallel, wherein the linear invisible detection line T is printed by the solution formed by the coupling of the copper ions and carrier protein, and the linear invisible control line C is printed by the goat anti-mouse IgG solution. The colloidal gold sensitization chromatography test strip for quickly detecting the copper ions, provided by the invention, has the advantages of strong specificity and high sensitivity, can detect the residue of the copper ions more quickly, sensitively, simply and conveniently, is lifelike, intuitive, accurate, simple and clear in result determination, is not easy to appear human misjudgment such as false positive, false negative and the like, saves the expense, has wide application range, facilitates the popularization, and has wide market prospect and remarkable economic and social benefits.

The invention relates to immunogen and coatinggen of chloramphenicol and application thereof in collaurum test paper, and belongs to the technical field of immunology. The immunogen and the coatinggen are a product of hapten coupling carrier protein obtained by oxidizing the chloramphenicol through potassium permanganate. The hapten is similar to a chloramphenicol counterpart in the aspects of molecular structures, stereochemistry and electron distribution; and hapten molecules have active groups which are convenient to couple with a protein carrier, and the active groups do not influence the electron distribution of analyte molecules. After hemocyanin is coupled by the hapten, organisms can generate antibodies with high valence and good specificity aiming at the chloramphenicol, and the antibodies are adsorbed to glass cellulose membranes; and the coatinggen constructed by coupling human serum protein can be adsorbed to nitrocellulose membranes and is used for constructing the chloramphenicol collaurum test paper.

The invention belongs to the technical field of clinical immunology, and discloses a monoclonal antibody prepared from African swine fever virus truncated protein p54 and application of the monoclonalantibody. The monoclonal antibody clone antibody 4-4C is finally obtained by immunizing the effectively truncated African swine fever virus protein p54 and systematically screening hybridoma. The obtained monoclonal antibody can react with the African swine fever totivirus to meet the basic requirements of the monoclonal antibody; in addition, the monoclonal antibody is good in specificity and has no cross reaction with other proteins of the African swine fever virus; in addition, after the monoclonal antibody is detected by Elisa, the titer of the monoclonal antibody reaches up to 1600000; finally, the monoclonal antibody has a blocking effect, the established blocking Elisa detection method has a detection effect equivalent to that of a Spanish detection kit recommended by OIE, the detection time is shortened, and the monoclonal antibody has the capacity of detecting the antibody level of African swine fever for pig farms.