30 results about "Heparan Sulfate Proteoglycan" patented technology

The invention discloses a tissue-engineered bone cartilage composite scaffold and a preparation method thereof, belonging to the technical field of biomaterials. The scaffold is of a multilayer integrated structure and comprises a cartilage tissue scaffold layer, a cartilage tissue calcified layer, a porous cell isolation membrane and a bone tissue scaffold layer. The cartilage tissue scaffold layer is inoculated with cartilage cells, and growth factors for promoting formation of cartilage are introduced to promote growth of cartilage cells. Main raw materials like heparan sulfate proteoglycan with good biocompatibility and degradability are selected and used and the method consisting of a cross-linking reaction, freeze drying, directional pore formation, compounding of multiple layers, overall integration and the like are carried out so as to obtain the functionalized multilayer integrated tissue-engineered bone cartilage composite scaffold with good mechanical properties. According to the invention, micro-nano hydroxyapatite crystal, degradable non-stoichiometric polyethylene glycol / poly(epsilon-caprolactone) nanometer coaxial short fiber, RGD-grafted heparan sulfate proteoglycan / oxidized sodium alginate and N-succinyl chitosan are compounded together to prepare the composite scaffold; and the composite scaffold is mainly used for restoration of full-thickness defects of articular cartilage and subchondral bone.

The invention discloses cross-linked hydrogel for muscle stem cell culture as well as a preparation method and application of the cross-linked hydrogel, and belongs to the technical field of biological food materials. The preparation method comprises the following steps: dissolving collagen to prepare a solution, then adding a certain amount of alginate and heparan sulfate proteoglycan, and uniformly mixing the alginate and heparan sulfate proteoglycan with the collagen solution; and adding epsilon-PL and TG enzyme into the solution, uniformly conducting stirring, and putting slurry into a mold for crosslinking to obtain the hydrogel. According to the hydrogel disclosed by the invention, collagen, polylysine and heparan sulfate proteoglycan are connected through TG enzyme to form covalent crosslinking, and a compact three-dimensional 'egg box' net structure is formed through physical electrostatic interaction between polylysine and alginate. By utilizing the hydrogel, the absorption of the muscle stem cells on nutrient substances can be enhanced, and the growth of the muscle stem cells is facilitated. The bi-crosslinked network hydrogel has the potential to be used as a scaffold for muscle stem cell growth of stem cell culture meat.

The present invention relates to an in vitro method for identifying a molecule, which interferes with the interaction between a glial cell-line derived neurotrophic factor family ligand (GFL) and a heparan sulfate proteoglycan (HSPG), by screening a library of molecules against a matrix anchored complex comprising at least one immobilized glial cell-line derived neurotrophic factor family ligand (GFL) and at least one heparan sulfate proteoglycan (HSPG), wherein the interfering molecule is isolated based on its capacity to replace a glial cell-line derived neurotrophic factor family ligand (GFL) in said anchored complex. The invention also relates to a complex for identifying such a molecule. The invention also relates to methods for preventing or delaying a neurodegenerative process as well as to method for prophylactic treatment or treatment of a disorder in the nervous system.

Provided herein are improved rAAV (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of ocular disorders or CNS disorders wherein the rAAV comprise one or more substitutions of amino acids that interact with heparan sulfate proteoglycan. The invention provides methods for improved transduction of retinal cells and methods for treating ocular diseases with improved compositions of rAAV particles. Further provided herein are improved recombinant adeno-associated virus (rAAV) (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of disorders of the CNS. The invention provides methods for delivering the rAAV to the CNS, methods for treating disorders of the CNS with improved compositions of rAAV particles, and kits for delivering the rAAV to the CNS and / or treating a CNS disorder.

Provided herein are improved rAAV (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of ocular disorders or CNS disorders wherein the rAAV comprise one or more substitutions of amino acids that interact with heparan sulfate proteoglycan. The invention provides methods for improved transduction of retinal cells and methods for treating ocular diseases with improved compositions of rAAV particles. Further provided herein are improved recombinant adeno-associated virus (rAAV) (e.g., rAAV2, rAAVrh8R, etc.) for enhanced gene therapy of disorders of the CNS. The invention provides methods for delivering the rAAV to the CNS, methods for treating disorders of the CNS with improved compositions of rAAV particles, and kits for delivering the rAAV to the CNS and / or treating a CNS disorder.

The invention relates to the field of daily chemicals and plant extracts, in particular to application of a bletilla striata(Thunb.)Rchb.f extract. The bletilla striata(Thunb.)Rchb.f extract has the ability of improving the expression of basilar membrane related genes and anti-aging related genes, and can effectively improve the expression of anti-aging effect related genes hbegf (heparin bindingEGF like growth factor), csf2 (colony stimulating factor 2) and egfr (epidermal growth factor receptor), and a basilar membrane stabilization related gene hspg2 (heparan sulfate proteoglycan) at the content of 0.01 percent. Thus, the bletilla striata(Thunb.)Rchb.f extract can be used for preparing external skin agents, and can serve as an anti-aging active component for firming a skin structure orstabilizing a dermal-epidermis junction.

Polypeptides of the neuregulin (NRG) heparin binding domain (N-HBD) and nucleic acids coding therefor are disclosed. In particular, fusion polypeptides are produced that comprise, as a targeting structure, a N-HBD polypeptide, fragment, homologue or functional derivative and a protein to be targeted. This is fused to a polypeptide or peptide being targeted (Ptrg) to cell surfaces rich in heparan sulfate proteoglycans to either activate or inhibit interactions at tyrosine kinase receptors. Such products are used to treat diseases or conditions where either agonism or antagonism at tyrosine kinase receptors has beneficial effects, including cancer and a multitude of diseases of the nervous system.

The invention discloses a bioactive material capable of efficiently combining and stimulating bFGF activity function and application. The invention also discloses application of the bioactive materialin preparation of a product for treating myocardial infarction. The bioactive material comprises an extracellular matrix molecular material, and the extracellular matrix molecular material comprisesheparan sulfate proteoglycan. According to the bioactive material, binding of heparan sulfate proteoglycan to basic fibroblast growth factor has high efficiency, good stability, and can greatly protect the basic fibroblast growth factor from being hydrolyzed by biological enzymes, and further promote biological activity of the basic fibroblast growth factor, etc. In addition, the bioactive material is used as a carrier of basic fibroblast growth factors, can well help reduce scar accumulation after myocardial infarction, relieve ventricular hypertrophy caused by injury and provide help for cardiac function recovery, and also provides a new thought for drug loading mode change of clinical myocardial infarction treatment.

A method for enhancing chondrogenesis in a subject in need thereof comprises administering to the subject an effective amount of a pharmaceutical composition comprising a growth factor bound to hyaluronic acid (HA)-based hydrogel particles (HGPs). The hyaluronic acid (HA)-based hydrogel particles (HGPs) are conjugated with a heparan sulfate proteoglycan (HSPG). Related medicaments and methods for preparing the medicaments are provided.

The invention discloses a fusion protein of liver-targeting peptide and recombinant human endostatin, its preparation method and application. The present invention selects 19 amino acids that can be specifically combined with the receptor--heparan sulfate proteoglycan HSPG on the surface of liver cells in the conserved I region of the N-terminal of the Plasmodium circumsporozoite protein CSP, and uses genetic engineering to fuse it in The carboxy-terminus of the novel human endostatin nhES with 9 amino acid sequences (MGGSHHHHH) added to the amino-terminus, and the liver-targeting peptide and recombinant human endostatin fusion protein (ES‑CSP) were prepared. The fusion protein is easy to purify, can specifically target the liver to inhibit the formation of new blood vessels, increase the local concentration of the drug at the lesion site, reduce the systemic dosage, and reduce its toxic and side effects. It provides ideas and scientific basis for the clinical development of drugs that target angiogenesis to treat liver cancer, and has great scientific significance and practical value for further improving the treatment level of liver cancer in my country.

The invention discloses a cross-linked hydrogel used for culturing muscle stem cells, a preparation method and application thereof, and belongs to the technical field of biological food materials. In the present invention, collagen is dissolved to form a solution, and then a certain amount of alginate and heparan sulfate proteoglycan is added to mix it with the collagen solution; then ε-PL and TG enzyme are added to the solution, stirred evenly, and The slurry is placed in a mold to cross-link, resulting in a hydrogel. The hydrogel of the present invention connects collagen, polylysine and heparan sulfate proteoglycans through TG enzyme to form covalent crosslinks, and through the physical electrostatic interaction between polylysine and alginate to form Dense three-dimensional "egg carton" network structure. The use of this hydrogel can enhance the absorption of nutrients by muscle stem cells and facilitate their growth. Double-crosslinked network hydrogels have potential as scaffolds for muscle stem cell growth in stem cell culture meat.

Provided is a method for inducing differentiation of pluripotent stem cells into somatic cells using a medium containing heparin binding growth factor, characterized by comprising contacting startingcells with a conjugate in which a laminin E8 fragment is bound to a fragment containing the growth factor-binding site of heparan sulfate proteoglycan. According to the present invention, differentiation of pluripotent stem cells into arbitrary somatic cells can be induced at a high efficiency.

The invention discloses a tissue engineering osteochondral composite support and a preparation method thereof, belonging to the technical field of biomaterials. The support is a multi-layer integrated structure, which is composed of a cartilage tissue support layer, a cartilage tissue calcification layer, a porous cell separation membrane and a bone tissue support layer. The cartilage tissue scaffold layer is inoculated with chondrocytes and introduced growth factors that promote chondrocyte formation to promote the growth of chondrocytes; the main raw materials such as heparan sulfate proteoglycan with good biocompatibility and degradability are selected, and cross-linking reaction, freeze-drying, and directional Pore-forming, multi-layer composite, complete integration and other methods to obtain functional multi-layer integrated tissue engineering osteochondral composite scaffold with good mechanical properties. The invention adopts micro-nano hydroxyapatite crystals and degradable non-stoichiometric polyethylene glycol / polyε-caprolactone nano-coaxial short fibers and heparan sulfate proteoglycan / oxidized sodium alginate grafted with RGD Compounded with N-succinyl chitosan, it is mainly used for repairing full-thickness defects of articular cartilage and subchondral bone.