43 results about "Globin binding" patented technology

The invention discloses a multi-species universal AGL-ELISA kit for differential diagnosis of foot and mouth disease virus infection. The multi-species universal AGL-ELISA kit comprises an ELISA plate coated with 8BF protein and recombinant protein [AGL]n undergoing enzyme labeling. The recombinant protein [AGL]n is a multi-copy recombinant protein formed by performing gene optimization and series connection on three protein structural domains which are a B structural domain in a staphylococcus aureus A protein combination structural domain, a C2 structural domain in a streptococcus protein G protein immune globulin combination structural domain and a B3 structural domain in a peptostreptococcus magnus protein L protein immune globulin combination structural domain, wherein n is 1, 2 or 3. The recombinant protein [AGL]n undergoes enzyme labeling, an enzyme labeling product can be combined with multi-species mammal immune globulin through western-blot and ELISA detection, can serve as a universal antibody for serologic detection of various mammals, and can replace species specific antibodies to perform multi-species anthropozoonosis detection. According to an AGL-ELISA, various animal foot-and-mouth disease infections can be detected, vaccine immunity, naturally injected animals and persistently infected animals can be identified.

The invention relataes to novel evolutionary immunoglobulin binding molecules, as well as their preparation methods and applications. The invention discloses separated evolutionary immunoglobulin binding molecules, which are proteins with amino acid sequences as shown by SEQ ID NO : 1, or conservative variant proteins with immunoglobulin binding activity. The invention also discloses the gene encoding, genetic engineering preparation methods and applications of immunoglobulin binding molecules. The disclosed immunoglobulin molecule broad-spectrum combined with various immuneglobulin shows high immunoglobulin whole molecule binding activity, and can be used in large-scale purification of genetic engineering antibodies, purification of natural antibodies and monoclonal antibodies, enzyme-linked immunosorbent assay, and immuno-chromatography and immunohistochemical methods for immune antibody detection and diagnosis.

A multimeric immunoglobulin-binding protein having improved properties as an affinity ligand for affinity chromatography, and an insoluble support inmmobilizing such a multimer. The immunoglobulin-binding protein is represented by the formula: (R1)_n-(R2)_m, or (R2)_m-(R1)_n. R2 is an immunoglobulin-binding domain including an amino acid residue that covalently bonds to an insoluble support upon immobilization reaction with the insoluble support, and R1 is an immunoglobulin-binding domain without containing an amino acid residue the presence of which in the sequence compared to when it is absent in the sequence reduces the immunoglobulin-binding activity of the support yielded by the immobilization reaction. The immunoglobulin-binding protein satisfies: (1) n is an integer of 5 to 9; (2) m is an integer of 1 or 2; (3) the n (R1) domains may or may not have the same sequence; and (4) the total number of domains (n+m) is 6 to 10.

The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

An immunoglobulin binding protein provided by the invention comprises variants of the B structural domain of a protein A, the C2 structural domain of a protein G and the B3 structural domain of a protein L or a combination of any one to three of the variants. The protein A, the protein G and the protein L have different binding characteristics and certain complementarity, so the immunoglobulin binding protein is an alkali-resistant multifunctional IBP molecule with wide reactivity and high affinity. The protein is purified by a three-step chromatography method, so that the protein quality canbe stabilized, the protein purity is more than 97%, the endotoxin level is lower than 1 Eu/mg, and the requirement of clinical protein is met. The stability of an immunoadsorption filler synthesized from the purified immunoglobulin binding protein is improved, so the use frequency of the filler can be effectively increased, and the service life of the filler is prolonged.

The invention provides an immunomagnetic bead-based time-resolved fluorescence immunoassay kit of cardiac myosin binding protein C (cMyBP-C). The kit includes a calibrator, immunomagnetic beads, immunofluorescence microspheres, analysis buffering liquid and wash liquid. The magnetic beads are coupled with antibodies, and the time-resolved fluorescence microspheres are coupled with the antibodies;thus the two parts form immunomagnetic beads-cMyBP-C antigen-immunofluorescence microsphere complexes with cMyBP-C antigens in a sample in a reaction tube after shaking incubation; a time-resolved instrument is used to determine intensity of fluorescence emitted thereby under excitation of ultraviolet light; and comparison of a standard curve is carried out to determine the amount of the cMyBP-C antigens in the sample. The kit of the invention greatly shortens reaction time, and improves efficiency and sensitivity of detection.

The present invention relates to a mutated immunoglobulin-binding protein having increased alkaline tolerance and, more specifically, to an immunoglobulin-binding protein in which, with respect to the A-domain of Staphylococcal protein A, or a functional variant thereof, an amino acid at a specific site is mutated and thereby exhibits an increased chemical stability at an alkaline pH value in comparison to a parental molecule. The present invention can provide an antibody-purifying immunoglobulin-binding protein ligand and matrix which have enhanced alkaline tolerance and accordingly enhanced stability in multiple times of alkaline cleaning.

The invention provides an evolved immunoglobulin binding molecule D-C-G3 characterized by having the amino acid sequence shown as SEQ ID NO:1. The invention also provides a cDNA sequence of the evolved immunoglobulin binding molecule D-C-G3 the code of which is shown as claim 1. The cDNA sequence is characterized in that the cDNA sequence is shown as SEQ ID NO:2. Moreover, the invention provides a preparation method of the evolved immunoglobulin binding molecule D-C-G3 as shown in claim 1. The evolved immunoglobulin binding molecule disclosed by the invention can bind total IgG of human and a plurality of animals and IgG of different subclasses in a broad-spectrum manner, and the bonding force of the evolved immunoglobulin binding molecule is higher than that of the immunoglobulin binding molecule compared with the prior art.

The invention discloses a recombinant Protein A protein and a preparation method of an affinity chromatography medium. The protein is a mutant of parent immune globulin-binding protein as defined by SEQ ID No 1 and SEQ ID No 2; and the preparation method of the affinity chromatography medium comprises the following steps: S1, synthesizing genes of the recombinant Protein A protein, S2, respectively constructing expression vectors by using the genes synthesized in the S1, S3, culturing, expressing and purifying to obtain a recombinant Protein A solution with a corresponding sequence of SEQ ID, and S4, preparing the affinity chromatography medium by using the recombinant Protein A. The preparation method has the advantages that the chemical stability of the Protein A in alkali liquor is greatly improved by redesigning an amino acid sequence, so that the Protein A with a specific binding effect on an antibody is provided, the Protein A has good chemical stability under an alkaline condition, the binding load of the recombinant Protein A and affinity chromatography medium is remarkably improved, in-place cleaning under 0.5-1.0 M NaOH can be tolerated, and the IgG binding load is 60-90 mg/ml.

The invention provides a multispecies universal detection protein with green fluorescence activity and application of the universal detection protein. The universal detection protein is characterizedin that the detection protein comprises a streptococcus protein G (SPG) segment and a fluorescent protein; and an IgG binding segment of an SPG gene is reconstructed, only a C3 region where the protein G can be specifically bound with the Fc terminal of an antibody IgG is retained, the C3 region is divided into three groups of C3, C3-D-C3 and C3-D-C3-D-C3, all the groups are connected to EGFP, anda prepared recombinant protein has the fluorescence activity and dual activity of binding to antibodies of different species. Disclosed evolutionary immunoglobulin binding molecules can widely bind to total IgG and IgG of different subclasses in humans and many animals in a wide spectrum mode, and compared with immunoglobulin binding molecules in the prior art, the binding force of the evolutionary immunoglobulin binding molecules is also higher than that of the existing immunoglobulin binding molecules.

This invention relates to high appetency immune globin combination molecule and its process method, wherein the method uses the structure units from the following fields: A, B, C, D, E fields of staphylococcus A protein; B1, B2, B3, B4, B5 structure fields of large digest streptococcus protein L; G1, G2 structure units of C and G streptococcus protein. The method connects the above structure units by random to recreate Ig combination molecule database expressed on the surface of the bacteriophage plaque. The immune globulin expresses the combination molecule to process appetency filtering for three to four times to get the aim combination molecule.