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Antibody directed against immunoglobulin-binding proteins of S. Aureus

A technology for staphylococcus and staphylococcus infection, which is applied in the field of monoclonal antibodies and can solve the problems of low correlation of SpA-E domains

Inactive Publication Date: 2018-05-22
ARSANIS BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

SpA-E domains were found to be less associated

Method used

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  • Antibody directed against immunoglobulin-binding proteins of S. Aureus
  • Antibody directed against immunoglobulin-binding proteins of S. Aureus
  • Antibody directed against immunoglobulin-binding proteins of S. Aureus

Examples

Experimental program
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Embodiment approach

[0484] 1. A monoclonal antibody that resists or neutralizes Staphylococcus aureus by specifically binding to the wild-type immunoglobulin binding protein (IGBP) of Staphylococcus aureus, comprising a cross-specific recognition of at least three IGBP domains CDR binding site, the IGBP domain is selected from protein A (SpA) domain and immunoglobulin binding protein (Sbi) domain SpA-A, SpA-B, SpA-C, SpA-D, SpA-E, The panel consisting of Sbi-I and Sbi-II, wherein the antibody binding to SpA-E has a K as determined by standard optical interferometry on F(ab)2 fragments D less than 5×10 -9 M affinity.

[0485] 2. The antibody according to definition 1, which recognizes at least three IGBP domains, preferably at least four, five or six IGBP domains.

[0486] 3. The antibody according to definition 1 or 2, which recognizes at least three IGBP domains, each K D less than 5×10 - 9 M, preferably identifying at least four or five IGBPs, each with less than 5 x 10 -9 M of K D .

...

Embodiment 1

[0624] Example 1: Generation of protein baits representing the IgG binding domains of SpA and Sbi.

[0625] The two IgG-binding proteins of Staphylococcus aureus, SpA and Sbi, are multidomain proteins and contain five and two IgG-binding domains, respectively ( image 3 ). The SpA domains A-E share 63-91% amino acid sequence identity, and the two Sbi domains share much less homology (27-32%) with each other or with the SpA domain ( Figure 4 A, B). These domains are highly conserved among different S. aureus strains, with SpA showing sequence variation in the C-terminus beyond the five IgG binding domains (SEQ ID: 121-134). To support the selection and characterization of monoclonal antibodies that bind to S. aureus IgG-binding proteins, the five IgG-binding domains of protein A (SpA) and the two domains of Sbi were recombinantly produced in E. coli Tuner DE3 cells. The DNA sequence of the seven domains was derived from the published genome sequence of S. aureus strain USA...

Embodiment 2

[0626] Example 2: Development of a human IqG library using yeast-expressed human IqG and an SpA IgG binding domain with wild-type sequence Human Monoclonal Antibody

[0627] SpA and Sbi binding monoclonal antibodies were selected from yeast based antibody presentation libraries developed according to WO2009 / 036379A2, WO2010105256 and WO2012009568.

[0628] Engineered to express full-length human IgG1 antibodies (with approximately 10 9 Diversity) yeast cell library, incubated with different concentrations of native full-length SpA (purified from Staphylococcus aureus NCTC 8325, purchased from Sigma, cat#P6031) or recombinant Spa-D domain, the former using the amino reactive reagent Sulfo - NHS-LC (Thermo Scientific) biotinylation and latter as described above. To avoid non-specific binding by the Fab domain, all VH3 germline sequences were excluded from the library for selection. Yeast cells expressing antibodies with the ability to bind these baits were isolated in seve...

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Abstract

A monoclonal antibody that counteracts Staphylococcus aureus by specifically binding to wild-type immunoglobulin-binding proteins (IGBP) of S. aureus comprising a cross-specific CDR binding site recognizing at least three of the IGBP domains selected from the group consisting of Protein A (SpA) domains and immunoglobulin-binding protein (Sbi) domains SpA-A, SpA-B, SpA-C, SpA-D, SpA-E, Sbi-I, and Sbi-ll, wherein the antibody has an affinity to bind SpA-E with a KD of less than 5x10-9M as determined by a standard optical interferometry method for a F(ab)2 fragment, and preferably binds to wt SpAequally or better compared to mutant SpA-KKAA that lacks binding to IgG Fc or VH3.

Description

technical field [0001] The present invention relates to monoclonal antibodies against Staphylococcus aureus by specifically binding to the immunoglobulin binding protein (IGBP) of Staphylococcus aureus. Background technique [0002] Staphylococcal surface protein A (SpA or protein A) and the staphylococcal binding agent of IgG (Sbi) are multifunctional virulence factors that interact with several human proteins and primarily function as immune evasion molecules (Falugi, 2013; Smith, 2011 ). It is proposed that SpA and Sbi protect S. aureus from phagocytosis by binding to the Fc portion of immunoglobulins. SpA also binds the VH3-type Fab region of antibodies, leading to cross-linking of the B-cell receptor (BCR) leading to non-antigen-specific B-cell activation and depletion. Suppression of the adaptive immune response to staphylococcal infection has shown convincing results in murine models (Sasso, 1991; Goodyear and Silverman, 2004 and 2005). [0003] SpA is a cell wall-...

Claims

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Application Information

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IPC IPC(8): C07K16/12C12N15/13A61K39/40A61P31/04G01N33/569
CPCC07K16/1271G01N33/56938C07K2317/92C07K2317/34C07K2317/33C07K2317/21A61P11/00A61P17/00A61P17/02A61P19/02A61P19/08A61P27/02A61P29/00A61P31/04A61P7/00A61P9/00A61K2039/505A61K39/40C07K2317/24C07K2317/55C07K2317/565C07K2317/76C07K2319/01
Inventor E·纳吉A·巴达罗H·劳哈L·斯图利克I·米尔基娜M·B·巴托斯N·尼尔森
Owner ARSANIS BIOSCI
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