30 results about "Gene map" patented technology

Recombinant respiratory syncytial virus (RSV) having the position of genes shifted within the genome or antigenome of the recombinant virus are infectious and attenuated in humans and other mammals. Gene shifted RSV are constructed by insertion, deletion or rearrangement of genes or genome segments within the recombinant genome or antigenome and are useful in vaccine formulations for eliciting an anti-RSV immune response. Also provided are isolated polynucleotide molecules and vectors incorporating a recombinant RSV genome or antigenome wherein a gene or gene segment is shifted to a more promoter-proximal or promoter-distal position within the genome or antigenome compared to a wild type position of the gene in the RSV gene map. Shifting the position of genes in this manner provides for a selected increase or decrease in expression of the gene, depending on the nature and degree of the positional shift. In one embodiment, RSV glycoproteins are upregulated by shifting one or more glycoprotein-encoding genes to a more promoter-proximal position. Genes of interest for manipulation to create gene position-shifted RSV include any of the NS1, NS2, N, P, M, SH, M2(ORF1), M2(ORF2), L, F or G genes or a genome segment that may be part of a gene or extragenic. A variety of additional mutations and nucleotide modifications are provided within the gene position-shifted RSV of the invention to yield desired phenotypic and structural effects.

Recombinant respiratory syncytial virus (RSV) having the position of genes shifted within the genome or antigenome of the recombinant virus are infectious and attenuated in humans and other mammals. Gene shifted RSV are constructed by insertion, deletion or rearrangement of genes or genome segments within the recombinant genome or antigenome and are useful in vaccine formulations for eliciting an anti-RSV immune response. Also provided are isolated polynucleotide molecules and vectors incorporating a recombinant RSV genome or antigenome wherein a gene or gene segment is shifted to a more promoter-proximal or promoter-distal position within the genome or antigenome compared to a wild type position of the gene in the RSV gene map. Shifting the position of genes in this manner provides for a selected increase or decrease in expression of the gene, depending on the nature and degree of the positional shift.

The invention provides a new method for accurately estimating physical and mechanical parameters of a geotechnical material, which comprises the following steps: acquiring prior gene characteristics: acquiring historical big data of physical and mechanical parameters of geotechnical materials in different areas and different groups, and performing statistical analysis according to the historical big data to obtain prior gene characteristics; carrying out posterior gene feature establishment: carrying out statistical analysis on physical and mechanical parameter test values of the newly added geotechnical material obtained in specific engineering detection to obtain posterior gene features; and accurately estimating the values of the mechanical parameters of the geotechnical material, namely accurately estimating the values of the corresponding parameters by using a Bayesian estimation method based on the prior gene characteristics and the posterior gene characteristics. According to the method, by means of the biological concept, the two concepts of the gene feature and the gene map of the geotechnical material for describing the overall physical and mechanical characteristics of the specific geotechnical material in a certain space range are put forward for the first time, and the prior gene feature and the posterior gene feature are combined; and the Bayesian estimation theory is used for rapidly and accurately estimating the geotechnical material parameters, so that the parameter estimation efficiency and precision are improved.

The invention discloses a construction method for a specific cancer differential expression gene regulation and control network.The method includes the following steps of firstly, constructing a framework gene interaction network according to function similarity weight numbers between genes; secondly, conducting module division on the framework gene interaction network through a segmenting method; thirdly, screening out differential expression genes through complete genome methylation data; fourthly, classifying the screened-out differential expression genes according to functions; fifthly, using all the differential expression genes mapped to each same function module as a function classification; sixthly, constructing the regulation and control network of all the genes in each function classification function; seventhly, conducting sub-network splicing under guidance of a framework network.The calculation complexity is greatly reduced, and high precision is achieved.

The invention provides a method for storing and restoring a gene sequence, a computer storage medium and an electronic device. The method for storing and restoring a gene sequence includes the following steps: S1, uploading the gene sequence, and setting a restoration password; S2, setting the The gene sequence of the recovery password is stored as a gene picture in picture format, and a positioning code is added during the storage process; S3, uploading the gene picture, and searching for the positioning code from the gene picture to determine the The starting position and reading direction of the gene sequence recorded in the gene picture; S4, input the password, and judge whether the password matches the restored password; S5, when the password matches the restored password, set The gene picture is restored to the gene sequence. The method for storing and restoring gene sequences according to the embodiments of the present invention can effectively reduce the trial-and-error time when gene pictures are restored to gene sequences, improve the efficiency of gene sequence restoration, and improve user experience.

The embodiment of the present invention discloses a method and device for implementing malicious traffic detection based on deep learning. The method may include: obtaining the traffic session of malicious code through dynamic sandbox technology; mapping the traffic session of malicious code into a gene map and extracting the map features, using the map features of traffic sessions to cluster, and mark the clustering results as malicious code families; use the marked genetic maps of malicious code families to train the preset deep learning model to establish a malicious traffic detection model; use malicious The traffic detection model detects the real-time traffic of the network to realize malicious traffic detection. Through this embodiment scheme, many problems existing in the current detection technology, such as difficulty in manual feature extraction, privacy leakage, difficult identification of encryption and confusion, manual extraction of machine learning features, etc., are solved to a certain extent, and the robustness is high, and it has Fast, high accuracy, low false alarm rate, cross-platform detection, etc.

The invention relates to the field of wheat genetics and its molecular breeding, in particular to the development and application of functional markers of the powdery mildew resistance gene of the goat grass. The present invention is effective in anti-powdery mildew gene of goat grass Ml2147 On the basis of map-based cloning, using the specific structural sequence of its candidate gene, developed SDAU‑2147 Functional markers and verification using more than 180 natural germplasms of A. tachyphylla. The results prove that the functional marker provided by the present invention has the characteristics of fast, direct, high-throughput, etc., and has high specificity. Worked on wheat powdery mildew resistance breeding.

The invention relates to the field of wheat genetics and molecular breeding thereof, in particular to development and application of a function mark of a powdery mildew resistant gene of aegilops tauschii. On the basis that the powdery mildew resistant gene M12147 of aegilops tauschii is subjected to map position clone, special structure sequences of candidate genes are used, a function mark SDAU-kasp2147 is developed, and verification is conducted by using 180 or more parts of aegilops tauschii natural germplasm and genetic offspring materials. The result proves that the function mark has theadvantage that the function mark is quick, direct and high in flux, is high in specificity, can complete screening and phenotype prediction work of the disease-resistant gene in mass materials in a short time, and can effectively serve for breeding work of wheat powdery mildew resistance.

The invention provides a gene sequence processing method, a computer storage medium and electronic equipment. The gene sequence processing method includes the following steps: S1, uploading the gene sequence, and setting a recovery password; S2, setting the recovery password The gene sequence is stored as a gene picture in picture format; S3, upload the gene picture, input a password, and judge whether the password matches the recovery password; S4, when the password matches the recovery password , restore the gene picture to the gene sequence. According to the gene sequence processing method of the embodiment of the present invention, when storing the gene sequence, by converting it into an image format for storage, the space occupied by data storage can be greatly reduced, the speed of gene sequence transmission can be improved, and the gene sequence In the process of converting to pictures, encrypting them can effectively improve information security.

The invention relates to a technological method for rapidly and finely positioning rice germplasm resource genes. The method mainly comprises the following steps: (1) selecting a rice variety with wide hereditary variation as a donor, and carrying out hybridization and backcrossing on the rice variety with the same recurrent parent respectively; selecting target traits including high yield, drought control, salt resistance and the like from a random population of a backcrossing introgressive line, so as to obtain a selected introgressive line population with the target traits; (2) carrying out non-target trait phenotype inspection on the multi-parent selected introgressive line population; positioning QTL (Quantitative Trait Locus) influencing non-target traits by utilizing a gene type and a phenotype, which are obtained by high-throughput sequencing, through genome-wide association analysis; (3) identifying a recombinant strain in a QTL confidence interval by adopting a high-density SNP (Single Nucleotide Polymorphism) gene type, and selectively inducing the recombinant strain with low frequency and combining a phenotype value to further finely position a QTL. The method is suitable for carrying out quantity trait QTL positioning and fine positioning on a multi-parent backcrossing population subjected to high-density sequencing; the gene map-based cloning time is shortened and a molecular breeding process is greatly accelerated.

The invention discloses a method for establishing a genetic map of identifying the true and false of baijiu. According to the method, DNA in the baijiu is extracted, amplification is conducted througha random primer polymerase chain reaction technology, separation and color development are conducted through gel electrophoresis, and the genetic map is drawn and used for identifying the true and false of the baijiu. According to the identification technology, the technical support can be provided for all baijiu production factories and quality monitoring and market supervision departments at all levels, thereby allowing the factories and the departments to take action actively, quickly and accurately during rights protection and law enforcement, and protecting the legitimate rights and interests of enterprises and consumers.