A method for rapid and fine mapping of rice genes

Abstract

The invention relates to a technological method for rapidly and finely positioning rice germplasm resource genes. The method mainly comprises the following steps: (1) selecting a rice variety with wide hereditary variation as a donor, and carrying out hybridization and backcrossing on the rice variety with the same recurrent parent respectively; selecting target traits including high yield, drought control, salt resistance and the like from a random population of a backcrossing introgressive line, so as to obtain a selected introgressive line population with the target traits; (2) carrying out non-target trait phenotype inspection on the multi-parent selected introgressive line population; positioning QTL (Quantitative Trait Locus) influencing non-target traits by utilizing a gene type and a phenotype, which are obtained by high-throughput sequencing, through genome-wide association analysis; (3) identifying a recombinant strain in a QTL confidence interval by adopting a high-density SNP (Single Nucleotide Polymorphism) gene type, and selectively inducing the recombinant strain with low frequency and combining a phenotype value to further finely position a QTL. The method is suitable for carrying out quantity trait QTL positioning and fine positioning on a multi-parent backcrossing population subjected to high-density sequencing; the gene map-based cloning time is shortened and a molecular breeding process is greatly accelerated.

Description

technical field [0001] The invention belongs to the fields of rice molecular breeding and molecular genetics, and specifically relates to a technical method for rapid and fine positioning of rice germplasm resource genes. Background technique [0002] Fine mapping is the only way for gene cloning. After using segregation populations or natural populations for phenotypic investigation and genotype identification, genetic linkage or association analysis is used to determine the interval where the QTL of the target trait is located, and fine mapping must begin. Gene fine mapping is usually to further narrow the QTL interval by continuously identifying recombinants in the interval of the gene until the mapping area contains one to several potential gene loci. When the number of gene loci is small enough, the use of bioinformatics and molecular biology methods can be considered to determine candidate genes, and then to verify the function of transgenes. [0003] It can be seen t...

AI Technical Summary

Problems solved by technology

Due to the randomness of the exchange of individual plants and exchange positions, the accuracy is relatively low, and it is often necessary to perform secondary fine positioning in the narrowed range

Each fine positioning needs to design a new SSR or other easy-to-verify markers, usually about 10, which is a huge workload for the identification of tens of thousands of samples

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