Application of group of genes related to prognosis of colon cancer

A technology of colon cancer and genes, applied in the direction of recombinant DNA technology, microbe determination/testing, DNA/RNA fragments, etc.

Active Publication Date: 2020-05-12
BERKELEY NANJING MEDICAL RES CO LTD +1
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Problems solved by technology

[0009] At present, there is no genetic detection and scoring system for the prognosis of colon cancer in China that can be used to guide the selection of treatment options. This invention is based on the comprehensive and unbiased genome-wide data of colon cancer so far, using a multi-step bioinformatics strategy, developed a set of 15 prognostic-related gene profiles completely different from the 7-gene

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  • Application of group of genes related to prognosis of colon cancer
  • Application of group of genes related to prognosis of colon cancer
  • Application of group of genes related to prognosis of colon cancer

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Embodiment 1

[0045] Compared with existing clinical application gene markers:

[0046] As mentioned above, Oncotype DX 7-genome is currently available in foreign markets to evaluate the prognosis of colon cancer. We compared a 15-gene scoring system to this system. We used the multivariate Cox regression analysis method to analyze the 7 genes in the previous 100 training sets, calculated the corresponding coefficients of each gene with the same method and took the average value, and calculated the TCGA 373 cases of colon cancer patients with the same formula prognostic score. The patients in the training group were sorted according to their prognosis scores, and the patients were divided into three groups with the same number of cohorts according to the scores, and the prognosis scores corresponding to the corresponding cut-off points were recorded, and the average value of the scores corresponding to each cut-off point was taken as the real cut-off point According to these two cut-off s...

Embodiment 2

[0048] To predict the prognosis of patients with clinical colon cancer:

[0049] The tumor tissues of patients with colon cancer received clinically were collected and RNA was extracted, and the tumor tissues may include fresh biopsy tissues, postoperative tissues, fixed tissues and paraffin-embedded tissues. Then, the expression levels of 15 genes in the prognostic scoring system were quantitatively detected with the kit developed by the present invention and corresponding instruments. The expression level of 15 genes is entered into the prognosis score formula that the present invention establishes:

[0050]

[0051] After calculating the patient's prediction score, the doctor predicts the patient's prognosis based on the score, such as the 5-year survival rate. At present, we have established a model through retrospective research and successfully verified it in different databases (Table 3). By calculating the prognostic score of all patients in the database, and rank...

Embodiment 3

[0053] Predicting response to the chemotherapeutic agent 5-fluorouracil in patients with clinical colon cancer: Survival of untreated patients with metastatic colon cancer is approximately 6 months. 5-Fluorouracil (5-Fu) is still the core drug in colon cancer chemotherapy. At present, colon cancer chemotherapy has a high recurrence rate and low effective rate. In order to reduce ineffective or excessive drug use and reduce medical costs, the present invention predicts the response of clinical colon cancer patients to the chemotherapy drug 5-FU through the implementation of the following scheme: for colon cancer received clinically Patients collect tumor tissue and extract RNA. Tumor tissue can include fresh biopsy tissue, postoperative tissue, fixed tissue, and paraffin-embedded tissue. Then, the expression level of the 15 genes of the prognostic scoring system was quantitatively detected with the kit developed by the present invention and corresponding instruments. The expre...

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Abstract

The present invention evaluates clinical prognosis of patients with colon cancer based on discovery of a group of 15 prognosis-related gene maps and a detection of expression levels of the group of the 15 prognosis-related gene maps in clinical tumor samples and according to prognosis correlation coefficients of these specific genes to calculate prognosis scores of the patients, and discloses a related application. The prognostic and predictive power of the system is twice that of the Oncotype DX (Genomic Health company) scoring system which is the only clinical application in the United States currently. According to the system and different detection technology platforms, corresponding 15 gene expression measurement kits are designed and developed. The system and product can assist in evaluating response of the patients with the colon cancer to therapeutic interventions to determine whether the patients benefit from chemical or targeted therapies for precise treatment based on individualized risks.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, and specifically relates to a group of human colon cancer-related prognosis genes and their application in clinical precision medicine. Background technique [0002] Colon cancer is one of the common malignant tumors of the digestive tract. Colon cancer ranks third in the incidence of tumors in the United States, with 140,000 new cases and 60,000 deaths each year. In China, with the change of people's living and eating habits, the incidence rate has been on the rise in the past two decades, tending to aging. The geographical distribution of morbidity and mortality in China is as follows: the incidence is higher in the eastern coastal areas than in the inland northwestern regions, and the highest incidence is in the middle and lower reaches of the Yangtze River in the economically developed areas. At present, the incidence of colon cancer is second only to lung cancer and ranks secon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/106C12Q2600/118C12Q2600/158
Inventor 王频邹晓平杭渤毛建华安托万·M·斯内德
Owner BERKELEY NANJING MEDICAL RES CO LTD
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