The invention discloses a vehicle path optimization method based on road network accessibility, and the method comprises the steps: firstly building a network data set of a research region, and obtaining a real road distance between research object points; then, under the condition that vehicle load constraints are met, carrying out coding operation on the distribution center and the client pointsin a natural number coding mode, and completing population initialization; after crossover and mutation operations are carried out on the population, adopting a roulette mode to carry out selection operation on the current population, screening out chromosomes with relatively high fitness to form a new population, and repeating the crossover, mutation and selection operations until an iteration termination condition is met; and finally, outputting the chromosome with the highest fitness in the population, i.e., the optimal path scheme for vehicle distribution. The invention provides a vehiclepath optimization method based on road network accessibility, which is more suitable for practical application.