30 results about "Endoproteinase Glu-C" patented technology

The present invention relates to a novel endoprotease, mutants thereof having binding but lacking or having reduced hydrolyzing activity, and use in methods of studying and isolating O-linked glycoproteins.

The present invention relates to a composition comprising hydrogels from functionalized hyaluronic acid derivatives, said hydrogels loaded with exogenous enzymes selected in the group consisting of prolyl endopeptidase (PEP) and endoprotease (EP) intended for the oral treatment of celiac disease. Specifically, this invention concerns a one-pot methodology useful to prepare methacrylic derivatives of hyaluronic acid, through the formation of a specific active group on hydroxyl groups of hyaluronic acid, the subsequent substitution of the inserted active group with ethylenediamine and finally, the reaction with methacrylic anhydride. The obtained methacrylic hyaluronic acid derivatives are used to prepare hydrogels through irradiation and loaded with exogenous enzymes selected in the group consisting of prolyl endopeptidase (PEP) and endoprotease (EP). The ability of prepared hydrogels to allow the enzyme release, as active form in simulated gastrointestinal fluids is proved.

The present invention relates to a method for producing a flavour composition comprising providing a slurry of yeast cell walls and contacting the slurry of yeast cell walls with a glucanase and with an endoprotease, followed by separating a liquid fraction by solid / liquid separation to provide the liquid flavour composition.

The invention discloses a pure draft beer film cleaning and regenerating enzyme preparation and a cleaning method using the same. The film cleaning and regenerating enzyme preparation designed according to the pure draft beer film plug substance composition comprises the following components: cleaning and regenerating enzyme I: 40-80% of pentosan enzyme (10000U / g), 10-50% of beta-glucanase (20000U / g) and 2-20% of mannase (10000U / g); and cleaning and regenerating enzyme II: 40-80% of proline endoproteinase (5U / g) and 20-60% of neutral proteinase (100000U / g). The optimized pure draft beer film cleaning and regenerating method comprises the following steps: conventional water washing and alkaline-process cleaning; cyclic cleaning by an enzyme process while keeping the temperature; impregnated cleaning by an enzyme process; water washing; alkaline-process cleaning; and integrity testing. The film cleaning and regenerating enzyme disclosed by the invention effectively solves the problem of film flux depression caused by the pure draft beer film plug substance, and enhances the film regeneration power and pure draft beer filtration capacity by more than 35%.

The invention discloses a method for preparing shellfish high F-value oligopeptides by a subcritical water-assisted enzymatic method, comprising the following steps: 1) subcritical water treatment: homogenate shellfish processing by-products and mix them with water, use subcritical water Extract with critical water and filter to obtain the extract; 2) Enzymolysis: Use endoprotease to enzymolyze the extract obtained in step 1), and then use exoprotease to enzymolyze to obtain enzymolysis; 3) Clarification: Remove the solid particles in the enzymatic hydrolysis solution obtained in step 2) to obtain a clarified enzymatic hydrolysis solution; 4) Decolorization: use activated carbon to decolorize the clarified enzymatic hydrolysis solution obtained in step 3) to obtain a decolorized enzymatic hydrolysis solution; 5) Refining: The decolorized enzymatic hydrolyzate obtained in step 4) is first subjected to ultrafiltration and then nanofiltration to obtain a high F value oligopeptide filtrate; and the high F value oligopeptide filtrate is dried. The invention effectively improves the F value of the product, greatly shortens the production time, reduces energy consumption, and reduces production costs.

The invention discloses a method for preparing and extracting Bacillus subtilis debittering aminopeptidase by fermentation, which belongs to the technical field of enzyme preparation and food additive. The invention uses the fermentation cylinder production condition of Bacillus subtilis Zj016 to collect fermentation liquor and obtain the product of debittering aminopeptidase by carrying out clarifying, ultrafiltration concentration and salting out on the fermentation liquor. The aminopeptidase produced by the selected Bacillus subtilis Zj016 is exopeptidase which can dissociate amino acid from a polypeptide chain amino terminal. Researched by the laboratory, the aminopeptidase cooperates with alkali protease to hydrolyze soybean protein isolate; the prepared soybean peptide does not havebitterness, and the contents of oligopeptide like dipeptide and tripeptide are higher. Besides, researches on the enzyme lines produced by the Bacillus subtilis show that the Bacillus subtilis only produces circumscribed prolease and does not have the activity of endo protease; moreover, the circumscribed prolease has a stronger hydrolysis capacity to the terminal amino acid with strong hydrophobicity. The research further explains that the invention can reduce or eliminate the bitterness generated during hydrolysis of the soybean protein.

The invention discloses a liquid chromatography-mass spectrometry technology-based protein-polysaccharide complex digestibility analyzing method. The liquid chromatography-mass spectrometry technology-based protein-polysaccharide complex digestibility analyzing method comprises in vitro digesting protein solution and protein-polysaccharide complex solution in simulative gastric juice; performing denaturation treatment on digested proteins and digested protein-polysaccharide complexes, removing low-molecular-weight peptide fragments through an ultrafiltration pipe, and further completely hydrolyzing large-fragment polypeptides retained inside the ultrafiltration pipe through a second endo protease with clear enzyme-digesting points except pepsase to obtain a number of polypeptide fragments;detecting the composition and abundance of the polypeptide fragments through the liquid chromatography-mass spectrometry technology; according to the abundance of the polypeptides digested by the second endo protease and the pepsase, determining the digestibility of the pepsase; further, according to the high-abundance polypeptides in the two types of peptide fragments in the protein-polysaccharide complex group, deducting the binding sites of proteins and polysaccharides.

The present invention relates to a method for producing a flavour composition comprising providing a slurry of yeast cell walls and contacting the slurry of yeast cell walls with a glucanase and with an endoprotease, followed by separating a liquid fraction by solid / liquid separation to provide the liquid flavour composition.

The invention discloses a preparation method of debittering and anti-oxidation oyster peptide, which comprises endo-proteolysis and exo-proteolysis, adding a debittering accelerator during the enzymatic hydrolysis; the debitterizing accelerator includes trehalose, debittering Agents may also include sodium carboxymethylcellulose. The present invention improves the exoenzyme debittering method by adding trehalose as a debittering agent in the enzymolysis process, effectively solves the problem of heavy bitterness in the antioxidant oyster peptide, and simultaneously improves the yield of the debittered oyster peptide, The time for debittering reaction is shortened. The addition of trehalose in the present invention also plays a role in inhibiting the Maillard reaction inhibitor, and a product with light color and simple taste is obtained. The method of the invention is low in cost, simple in operation, does not need to add new equipment on the basis of existing technology, and is suitable for industrial production of antioxidant oyster peptide.