Method for extracting low molecular weight active collagen peptide from pigskin

A technology of collagen peptide and low molecular weight, applied in the field of deep processing of pigskin, can solve the problems of low biological activity, complex production process, and low yield of collagen peptide, so as to optimize process steps, improve production efficiency, and improve production efficiency. rate effect

Inactive Publication Date: 2013-02-06
HUZHOU JIAMEI BIOCHEM PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adopts the technology of enzymatic hydrolysis twice, the production process is complicated, and the fat removed by the centrifuge is granular, and the fat particles still have collagen, the yield of collagen peptide is low, and the biological activity is low

Method used

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  • Method for extracting low molecular weight active collagen peptide from pigskin

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Comparison scheme
Effect test

Embodiment 1

[0021] The method for extracting low-molecular-weight active collagen peptides from pigskin comprises the following steps:

[0022] 1. the method for extracting low molecular weight active collagen peptide from pigskin, comprises the following steps:

[0023] Use fresh pigskin as raw material, wash and cut into meat strips, add deionized water of 5 times the mass of the raw material;

[0024] Heat up to 40°C, add compound protease with raw material quality 2‰ , carry out enzymatic hydrolysis;

[0025] complex protease When the meat strips disintegrate and become loose, add a compound protease with a raw material quality of 5‰ , further enzymatic hydrolysis;

[0026] After the enzymolysis is complete, raise the temperature to 90-95°C, keep it for more than 15 minutes, inactivate the enzyme, and let it stand until the enzymolysis solution is completely separated, and the lower layer of clear liquid is separated;

[0027] Add activated carbon to the liquid ...

Embodiment 2

[0033] The method for extracting low-molecular-weight active collagen peptides from pigskin comprises the following steps:

[0034] 1. the method for extracting low molecular weight active collagen peptide from pigskin, comprises the following steps:

[0035] Use fresh pigskin as raw material, wash and cut into meat strips, add deionized water of 7 times the mass of raw material;

[0036] Raise the temperature to 42°C, add a compound protease with a raw material quality of 3‰ , carry out enzymatic hydrolysis;

[0037] complex protease When the meat strips disintegrate and become loose, add a compound protease with a raw material quality of 4‰ , further enzymatic hydrolysis;

[0038] After the enzymolysis is complete, raise the temperature to 90-95°C, keep it for more than 15 minutes, inactivate the enzyme, and let it stand until the enzymolysis solution is completely separated, and the lower layer of clear liquid is separated;

[0039] Add activated carbon...

Embodiment 3

[0045] 1. the method for extracting low molecular weight active collagen peptide from pigskin, comprises the following steps:

[0046] Use fresh pigskin as raw material, wash and cut into meat strips, add deionized water with 8 times the mass of raw material;

[0047] Heat up to 45°C, add compound protease with raw material quality 4‰ , carry out enzymatic hydrolysis;

[0048] complex protease When the meat strips disintegrate and become loose, add a compound protease with a raw material quality of 3‰ , further enzymatic hydrolysis;

[0049] After the enzymolysis is complete, raise the temperature to 90-95°C, keep it for more than 15 minutes, inactivate the enzyme, and let it stand until the enzymolysis solution is completely separated, and the lower layer of clear liquid is separated;

[0050] Add activated carbon to the liquid that separates and flow out, filter after decolorization, and obtain the filtrate;

[0051] The filtrate is filtered and con...

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Abstract

The invention relates to the technical field of deep processing of pigskin, particularly relates to a method for extracting pigskin collagen peptide. The method uses fresh pigskin to serve as a raw material, and a pure biology method is used for obtaining the pigskin collagen peptide. The method for extracting low molecular weight active collagen peptide from the pigskin comprises the following steps of: taking the fresh pigskin to serve as the raw material, firstly conducting outer cutting on collagen in the pigskin by adopting aminopeptidase and carboxypeptidase; separating the collagen from other tissue elements in the pigskin, then the pigskin is in a scattered state, and using endo protease to conduct enzymolysis on protein to enable the protein to form micromolecular protein peptides. Different protein peptides are scientifically selected in different stages, processing steps are optimized, production efficiency is improved, yield of the collagen peptide is promoted, and activity of the collagen peptide is improved.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of pigskin, in particular to a method for extracting pigskin collagen peptides, which is a method for obtaining pigskin collagen peptides by using fresh pigskin as a raw material by purely biological means. Background technique [0002] Pigskin contains about 70% to 75% of water, 21% to 23% of protein, 1% of fat and 0.5% of ash. The protein in pigskin is mainly collagen, with a content of 87.8%. Collagen has a unique three-strand superhelical structure. The three chains are parallel to each other and connected by hydrogen bonds between the chains. It has a very stable property. It is difficult to decompose it under general processing temperature and short-term heating, so it is difficult to be absorbed by the human body. It can be eaten directly The utilization rate is low; if collagen is hydrolyzed into polypeptides, the digestion and absorption rate can reach almost 100%, ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K4/12C07K1/14
Inventor 俞继荣
Owner HUZHOU JIAMEI BIOCHEM PRODS
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