Preparation method and application of drone pupa small-molecular antioxidant peptide

A technology for antioxidant peptides and small molecules, which is used in the preparation methods of peptides, chemical instruments and methods, and medical preparations containing active ingredients.

Inactive Publication Date: 2018-05-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the peptide prepared by this method is still a peptide with a large molecular weight (molecular weight of tens of thousands or even hundreds of thousands), not a small molecule peptide

Method used

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  • Preparation method and application of drone pupa small-molecular antioxidant peptide
  • Preparation method and application of drone pupa small-molecular antioxidant peptide
  • Preparation method and application of drone pupa small-molecular antioxidant peptide

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preparation example Construction

[0030] The invention provides a method for preparing a drone pupae small-molecule antioxidant peptide, comprising the following steps:

[0031] 1) Mix and homogenize the drone pupae powder with water, adjust the pH value of the homogenate to 6-7, mix it with a compound enzyme, and enzymolyze it at 50-60°C for 1-3 hours to obtain an enzymolysis solution, and the compound Enzymes include endoprotease and exoprotease; 2) Centrifuge the enzymatic hydrolyzate obtained in the step 1), filter the obtained supernatant to obtain a filtrate, and the speed of the centrifuge is 4000-5000rpm, The pore size of the filter cloth used for the filtration is 1-5 μm; 3) The filtrate obtained in the step 2) is subjected to the first step of ultrafiltration to separate the peptide segment to obtain an ultrafiltrate with a molecular weight of the peptide segment <1 ku, and the first step The molecular weight cut-offs of the ultrafiltration membranes used in the step ultrafiltration are 10ku, 5ku, 3k...

Embodiment 1

[0056] Grind the freeze-dried drone pupae into powder, pass through a 80-mesh sieve, add water 15 times the weight of the freeze-dried drone pupae, and homogenate, adjust the pH to 6.5 with dilute hydrochloric acid and low-concentration sodium hydroxide solution, add compound enzymes at 60°C Under enzymolysis for 2h, the enzymolysis solution was obtained (see figure 1 ), the compound enzyme is neutral protease and flavor protease, and every 1g of drone pupae powder is added with a compound enzyme whose total enzyme activity is 1760U, and the enzyme activity ratio of neutral protease and flavor protease is 3:1.

[0057] Centrifuge the enzymatic solution at 4000rpm for 15min, discard the upper layer of grease, take the supernatant and pass it through a 5μm filter cloth, and use the ultrafiltration membrane with a molecular weight cut-off of 1ku to perform the first step of ultrafiltration to separate the peptide segment to obtain the peptide segment For the ultrafiltrate with a ...

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Abstract

The invention provides a preparation method of drone pupa small-molecular antioxidant peptide and belongs to the technical field of functional foods. The method includes the steps of: mixing and homogenizing drone pupa powder with water, regulating pH value of the homogenized liquid, mixing the homogenized liquid with compound enzyme to perform enzymolysis, wherein the compound enzyme includes endo-protease and exo-protease, centrifugally separating and filtering the enzymolytic liquid to prepare a filtrate; perform first ultra-filtering separation to the filtrate to obtain peptide fragments;performing secondary ultra-filtering to remove salts; and performing pressure reduced concentration and freeze drying to obtain the drone pupa small-molecular antioxidant peptide. The method can reduce generation of bitter peptides in the enzymolytic liquid and increase content of the small-molecular peptides in the enzymolytic liquid. The molecular weight of the drone pupa small-molecular antioxidant peptide is less than 1 kU, and the peptide has high-active anti-oxidizing performance.

Description

technical field [0001] The invention relates to the technical field of functional food, in particular to a method for preparing a drone pupae small-molecule antioxidant peptide and an application of the drone pupae small-molecule antioxidant peptide. Background technique [0002] Drone pupae refers to the unfertilized eggs of the queen bee in the drone house, and the pupae that have been hatched, developed, and emerged by worker bees. It generally refers to the individual when the male bee develops to 22 to 23 days old. At this time, the pupa is mature, but not yet mature. Eclosion, which is extremely rich in nutrients, is a waste product from the production of royal jelly. Bee colonies in my country can produce more than 4000t of dried drone pupae every year. But for a long time, the bee pupae products produced in China are mainly bee pupae protein powder, which are only processed simply, or will be directly exported as raw materials. development and utilization. [0003...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P39/06A23L33/18
CPCA61K38/012A23L33/18C07K1/34C12P21/06
Inventor 刘睿盛金旺鲁群张久亮蔡昭龙
Owner HUAZHONG AGRI UNIV
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