Collagen small molecule peptide rich in dipeptide Hyp-Gly, preparation method and application thereof

A small molecule peptide and collagen technology, which is applied in the field of collagen small molecule peptide rich in dipeptide Hyp-Gly and its preparation, can solve the problems of destruction and low content of dipeptide Hyp-Gly, and can increase skin moisture and elasticity, The effect of small molecular weight and high content

Active Publication Date: 2022-02-11
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem of low content of dipeptide Hyp-Gly in current collagen peptide products, provide a kind of collagen small molecular peptide rich in dipeptide Hyp-Gly and its preparation method and application, using specific endoprotease Hydrolyze with exopeptidase step by step and control the directional release of Hyp-Gly, avoid excessive enzymatic hydrolysis on the destruction of the peptide bond on the Hyp-Gly dipeptide, thereby increasing the content of dipeptide Hyp-Gly in collagen peptide products, and at the same time improving Content of <1000Da Small Molecular Peptide Components

Method used

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  • Collagen small molecule peptide rich in dipeptide Hyp-Gly, preparation method and application thereof
  • Collagen small molecule peptide rich in dipeptide Hyp-Gly, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0034] A method for preparing a collagen small molecule peptide rich in dipeptide Hyp-Gly, comprising the steps of:

[0035] (1) Alkali washing: Soak the tilapia skin in 0.4% NaOH solution for 1.5h.

[0036] (2) Cleaning: Rinse the alkali-washed fish skin with a large amount of water until the pH of the cleaning solution drops to 7.0-8.5, stop cleaning, and drain the fish skin.

[0037] (3) Heat extraction: add 2 times water according to the mass of fish skin, heat treatment at 90° C. for 2 hours. After heat treatment, cool to about 40°C and adjust the pH to 7.5.

[0038] (4) The first step of enzymatic hydrolysis: add 0.3% neutral protease to the gelatin extract obtained in step (3) according to the mass of fish skin, enzymatically hydrolyze at 55°C for 6h, raise the temperature of the feed liquid to 95°C for 30min to inactivate the enzyme, and cool .

[0039] (5) The second step of enzymatic hydrolysis: add 0.3% flavor protease according to the mass of fish skin to the en...

Embodiment 2

[0044] A method for preparing a collagen small molecule peptide rich in dipeptide Hyp-Gly, comprising the steps of:

[0045] (1) Alkaline washing: Soak the tilapia skin in 0.3% NaOH solution for 2 hours.

[0046] (2) Cleaning: Rinse the alkali-washed fish skin with a large amount of water until the pH of the cleaning solution drops to 7.0-8.5, stop cleaning, and drain the fish skin.

[0047] (3) Heat extraction: add 2 times water according to the mass of fish skin, heat treatment at 80° C. for 3 hours. After heat treatment, cool to about 45°C and adjust the pH to 7.5.

[0048] (4) The first step of enzymatic hydrolysis: add 0.25% alkaline protease to the gelatin extract obtained in step (3) according to the mass of fish skin, enzymolyze it at 55°C for 6 hours, raise the temperature of the feed solution to 95°C and maintain it for 30 minutes to inactivate the enzyme, and cool .

[0049] (5) The second step of enzymolysis: add 0.15% aminopeptidase A to the enzymolysis solutio...

Embodiment 3

[0054] A method for preparing a collagen small molecule peptide rich in dipeptide Hyp-Gly, comprising the steps of:

[0055] (1) Alkali washing: Soak the tilapia skin in 0.5% NaOH solution for 1 hour.

[0056] (2) Cleaning: Rinse the alkali-washed fish skin with a large amount of water until the pH of the cleaning solution drops to 7.0-8.5, stop cleaning, and drain the fish skin.

[0057] (3) Heat extraction: add 2 times water according to the mass of fish skin, heat treatment at 90° C. for 2 hours. After heat treatment, cool to about 50°C and adjust the pH to 7.0.

[0058] (4) The first step of enzymatic hydrolysis: add 0.2% compound protease according to the weight of fish skin to the gelatin extract obtained in step (3), enzymatically hydrolyze at 50°C for 4 hours, raise the temperature of the feed solution to 95°C for 30min to inactivate the enzyme, and cool.

[0059] (5) The second step of enzymolysis: add 0.25% aminopeptidase A to the enzymolysis solution obtained in s...

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Abstract

The invention discloses a collagen small molecule peptide rich in dipeptide Hyp-Gly, a preparation method and application thereof, wherein the content of the dipeptide Hyp-Gly in the collagen small molecule peptide is 0.8% or above, and the peptide with the molecular weight smaller than 1000 Da accounts for 85% or above. According to the invention, specific endoprotease and exopeptidase are adopted for step-by-step hydrolysis, and directional release of Hyp-Gly is controlled, so that damage of excessive enzymolysis to peptide bonds on Hyp-Gly dipeptide is avoided so as to improve the content of dipeptide Hyp-Gly in a collagen peptide product and increase the content of small molecule peptide components smaller than 1000 Da; and the collagen small molecule peptide rich in dipeptide Hyp-Gly can be used for preparing functional food with beautifying and skin care effects.

Description

technical field [0001] The invention belongs to the field of biologically active peptides, and in particular relates to a collagen small molecule peptide rich in dipeptide Hyp-Gly and its preparation method and application. Background technique [0002] Collagen is the most abundant protein in mammals and widely exists in tissues such as skin, bones and blood vessels in animals. Among them, type I collagen accounts for 80-90% of the total collagen content, mainly distributed in tissues such as skin and tendon, and is also the most abundant protein in aquatic product processing waste (skin, bone and scale). Collagen peptide is a product obtained by hydrolyzing collagen by enzymatic or fermentation methods. It has a smaller molecular weight and is easier to be absorbed and utilized in the body. [0003] Skin aging is mainly caused by physiological factors and environmental factors. The aging caused by ultraviolet rays is called photoaging, which is the most important kind of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/22A23L33/18
CPCC12P21/06C07K1/36C07K1/34C07K1/22A23L33/18A23V2002/00A23V2200/318A23V2250/55Y02P20/55
Inventor 赵谋明刘琪郑淋刘晓影赵翊君
Owner SOUTH CHINA UNIV OF TECH
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