The invention relates to a method for extracting toosendanin from cortex meliae. The method comprises the following
processing steps: cleaning dry mature cortex meliae and then crushing the mature cortex meliae by a
pulverizer,
grinding the mature cortex meliae into
powder,
drying the
powder and adding a 60%
ethanol solvent; adjusting the pH value of the solution to 4-6, and adding
cellulase for enzymolysis; putting the extracting solution into a double-frequency ultrasonic extractor to obtain an enzymatic
hydrolysate; carrying out high-temperature inactivation on the enzymatic
hydrolysate to obtain an extracting solution; centrifuging, taking supernate, extracting by use of
chloroform, and
drying by use of
anhydrous sodium sulfate; carrying out suction
filtration, concentrating, adding
ethanol and heating for dissolving; dropping
petroleum ether until the mixture becomes a turbid matter; stewing for separating out a flavescent
solid, namely the toosendanin. According to the method, the cortex meliae is taken as the
raw material; the stacking effect of enzymolysis and a double-frequency ultrasonic wave is fully utilized; the toosendanin in the cortex meliae is dissolved to the greatest extent; the high-purity toosendanin can be obtained by virtue of extraction and
crystallization. The method has the advantages of being simple in equipment, low in extraction cost, high in extraction efficiency, mild in reaction condition, less in
solvent consumption, free of
pollution and the like.