Method for extracting asiaticoside from centella

A technology of asiaticoside and centella asiatica, which is applied in the field of natural plant extraction products, can solve problems such as no public literature reports, and achieve the effects of being beneficial to dissolution, high yield and simple process

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through literature search, do not see the public literature report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Centella asiatica is crushed, passed through a 80-mesh sieve, and 1kg of powder is taken, and the compound enzyme equivalent to 1% of centella asiatica powder (the weight ratio of cellulase, hemicellulase and pectinase is 0.5:0.3:0.2) is sprayed On it, wrapped with film, piled up in a dark room at a temperature of 35°C, and fermented for 24 hours. The fermented raw materials were added with 8 times of 50% ethanol, and placed in a dual-frequency ultrasonic extractor. The ultrasonic frequencies were 40KHz and 80KHz respectively, and the power was 400W, extract at 60°C for 60min, filter, concentrate, extract with petroleum ether-water, remove the lower layer, put it on an alumina column, elute with 10BV of 50% ethanol, concentrate the eluate, and recrystallize twice from methanol to obtain Centella asiatica Glycoside 10.2g, the purity is 90.5%, and the yield is 1.02%.

Embodiment 2

[0016] Centella asiatica is crushed, passed through a 80-mesh sieve, and 1kg of powder is taken, and the compound enzyme equivalent to 1.5% of centella asiatica powder (the weight ratio of cellulase, hemicellulase and pectinase is 0.5:0.3:0.2) is sprayed On it, wrapped with film, stacked in a dark room with a temperature of 40°C, fermented for 20 hours, added 6 times of 60% ethanol to the fermented raw materials, placed in a dual-frequency ultrasonic extractor, the ultrasonic frequencies were 40KHz and 80KHz, and the power was 600W, extract at 55°C for 80min, filter, concentrate, extract with petroleum ether-water, remove the lower layer, put it on an alumina column, elute with 12BV of 60% ethanol, concentrate the eluent, and recrystallize twice from methanol to obtain Centella asiatica Glycoside 12.2g, the purity is 94.1%, and the yield is 1.22%.

Embodiment 3

[0018] Centella asiatica is crushed, passed through a 80-mesh sieve, and 1kg of powder is taken, and the compound enzyme equivalent to 2% of centella asiatica powder (the weight ratio of cellulase, hemicellulase and pectinase is 0.5:0.3:0.2) is sprayed On it, wrapped with a film, piled up in a dark room at a temperature of 45°C, and fermented for 15 hours. The fermented raw materials were added with 5 times of 55% ethanol, and placed in a dual-frequency ultrasonic extractor. The ultrasonic frequencies were 40KHz and 80KHz, and the power was 500W, extract at 50°C for 90min, filter, concentrate, extract with petroleum ether-water, remove the lower layer, put it on an alumina column, elute with 15BV of 70% ethanol, concentrate the eluent, and recrystallize methanol twice to obtain Centella asiatica Glycoside 12.4g, the purity is 93.6%, and the yield is 1.24%.

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Abstract

The invention relates to a method for extracting asiaticoside from centella. The method for extracting the asiaticoside from the centella comprises the following processing steps: smashing raw materials, sieving by adopting a sieve with 80 meshes, fermenting by adopting compound enzyme, adding 50-60% ethyl alcohol with the amount being 5-8 times that of the raw material into the fermented raw material, placing the obtained mixture into a double-frequency ultrasonic extractor, extracting at the temperature of 50-60 DEG C for 60-90 minutes with ultrasonic frequencies being 40KHz and 80KHz and power being 400-600W, filtering, concentrating, carrying out petroleum ether-water extraction, taking a lower layer, and carrying out separation and purification by virtue of an alumina column, concentration and recrystallization, so that the asiaticoside is obtained. The method for extracting the asiaticoside from the centella is short in extraction time, high in yield, easy in operation, safe and reliable, high in efficiency and energy-saving and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting asiaticoside from centella asiatica, in particular to a method for extracting asiaticoside through compound enzyme fermentation and double-frequency ultrasonic waves, and belongs to the technical field of natural plant extraction products. Background technique [0002] Centella asiatica is the dry whole herb of Umbelliferae. Its nature is citrus, bitter and pungent. It has the effects of clearing away heat and promoting dampness, detoxifying and reducing swelling. Sores, injuries from falls and other diseases. Its main components are triterpenoid saponins such as asiaticoside and madecassoside, which can promote wound healing, stimulate granulation growth, promote epidermal keratinization, and help to form new connective tissue. Marks have obvious curative effect. [0003] Enzyme engineering technology, through the selection of appropriate enzyme preparations, such as cellulase, pectinase, protease, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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