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Method for extracting toosendanin from cortex meliae

A technology of toosendanin and azalea bark, which is applied in the field of preparation of high-purity lycopene, can solve the problems of standing waves, single-frequency ultrasonic sound field is not uniform, and affects the extraction effect, and achieves reduced dead angles, low cost, and simple equipment Effect

Inactive Publication Date: 2013-12-18
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the sound field of single-frequency ultrasound is not uniform enough, the transaction produces standing waves, which affects the extraction effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Wash the dry and mature neem bark, crush it with a pulverizer, grind it into powder, dry it at 80°C, add 60% ethanol solvent, and the solid-to-liquid ratio is 1:20; adjust the pH value of the solution to 4.0, and add 0.05% of Cellulase enzymatic hydrolysis; the extract was placed in a dual-frequency ultrasonic extractor, the ultrasonic frequencies were 25Hz and 40Hz, the ultrasonic power was 100W, the ultrasonic time was 50min, and the ultrasonic temperature was 60°C; the enzymolyzed solution was placed in In a water bath, inactivate at 80°C for 10 minutes to obtain the extract, centrifuge, and take the supernatant; extract the supernatant 3 times with chloroform, combine the extract, add anhydrous sodium sulfate to dry, filter with suction, concentrate, add 4BV of ethanol Heat to dissolve, drop petroleum ether until the mixture becomes turbid, stand still, and precipitate a light yellow solid, which is toosendanin, and the extraction rate is 3.01%.

Embodiment 2

[0018] Wash the dry and mature neem bark, crush it with a pulverizer, grind it into powder, dry it at 80°C, add 60% ethanol solvent, and the solid-liquid ratio is 1:25; adjust the pH value of the solution to 5.0, and add 0.03% of Cellulase enzymatic hydrolysis; the extract was placed in a dual-frequency ultrasonic extractor, the ultrasonic frequencies were 25Hz and 40Hz, the ultrasonic power was 120W, the ultrasonic time was 40min, and the ultrasonic temperature was 50°C; the enzymolyzed solution was placed in In a water bath, inactivate at 80°C for 10 minutes to obtain the extract, centrifuge, and take the supernatant; extract the supernatant 3 times with chloroform, combine the extract, add anhydrous sodium sulfate to dry, filter with suction, concentrate, add 6BV of ethanol 89%。 Heating to dissolve, drop in petroleum ether until the mixture becomes turbid, stand still, precipitate a light yellow solid, which is toosendanin, the extraction rate is 3.89%.

Embodiment 3

[0020] Wash the dry and mature neem bark, crush it with a grinder, grind it into powder, dry it at 80°C, add 60% ethanol solvent, and the solid-to-liquid ratio is 1:23; adjust the pH value of the solution to 6.0, and add 0.04% Cellulase enzymatic hydrolysis; the extract was placed in a dual-frequency ultrasonic extractor, the ultrasonic frequencies were 25Hz and 40Hz, the ultrasonic power was 150W, the ultrasonic time was 30min, and the ultrasonic temperature was 40°C; the enzymolyzed solution was placed in Inactivate in a water bath at 80°C for 10 minutes to obtain the extract, centrifuge, and take the supernatant; extract the supernatant 3 times with chloroform, combine the extract, add anhydrous sodium sulfate to dry, filter with suction, concentrate, add 5BV of ethanol 34%。 Heating to dissolve, drop in petroleum ether until the mixture becomes turbid, stand still, precipitate a light yellow solid, that is toosendanin, the extraction rate is 3.34%.

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Abstract

The invention relates to a method for extracting toosendanin from cortex meliae. The method comprises the following processing steps: cleaning dry mature cortex meliae and then crushing the mature cortex meliae by a pulverizer, grinding the mature cortex meliae into powder, drying the powder and adding a 60% ethanol solvent; adjusting the pH value of the solution to 4-6, and adding cellulase for enzymolysis; putting the extracting solution into a double-frequency ultrasonic extractor to obtain an enzymatic hydrolysate; carrying out high-temperature inactivation on the enzymatic hydrolysate to obtain an extracting solution; centrifuging, taking supernate, extracting by use of chloroform, and drying by use of anhydrous sodium sulfate; carrying out suction filtration, concentrating, adding ethanol and heating for dissolving; dropping petroleum ether until the mixture becomes a turbid matter; stewing for separating out a flavescent solid, namely the toosendanin. According to the method, the cortex meliae is taken as the raw material; the stacking effect of enzymolysis and a double-frequency ultrasonic wave is fully utilized; the toosendanin in the cortex meliae is dissolved to the greatest extent; the high-purity toosendanin can be obtained by virtue of extraction and crystallization. The method has the advantages of being simple in equipment, low in extraction cost, high in extraction efficiency, mild in reaction condition, less in solvent consumption, free of pollution and the like.

Description

technical field [0001] The invention relates to a preparation method of high-purity lycopene, in particular to a method for extracting lycopene by enzymatic method-ultrasonic wave. Background technique [0002] Neem bark is the dried bark and root bark of Neem or Neem of Meliaceae. The Chinese Pharmacopoeia believes that the bark of neem is bitter, cold, poisonous, and belongs to the liver, spleen, and stomach meridian. Neem and Neem are widely distributed in our country and are very rich in resources. Toosendanin is one of the important active ingredients in neem with a clear structure, which has a variety of physiological activities, such as blocking the normal transmission between neuromuscular junctions, inhibiting the respiratory center, anti-botulinum, anti-oxidation, anti-bacterial and anti-inflammatory, Antiviral and antitumor effects, etc., so the research and preparation of toosendanin have broad development prospects and application value. [0003] At present, ...

Claims

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Application Information

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IPC IPC(8): C07J71/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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