The invention relates to an extraction and separation technology and a production process of limonin glucoside. A limonin substance is a triterpene ingredient existing in rutaceae and meliaceae plants, has anti-inflammatory and analgesic effects and an effect of resisting various cancer cells and is mainly applied to food additives and cosmetics. But the flavor of the limonin substance is bitter, and the solubility is poorer, so that the wide application of the limonin substance is limited. The limonin glucoside is an advanced glycation end product of limonin, has the characteristics of no foreign flavor, good solubility, solution stability and the like and has a wider market prospect. An extraction process of the limonin glucoside is as follows: crushing and sieving, reflux extraction and concentration, alcohol precipitation, impurity removal, resin separation, decolorization, column chromatography and detection. The natural content of the limonin glucoside is about 0.02%. By the aid of the extraction and separation technology and the production process of limonin glucoside and a method, a target product can be effectively enriched, products with different specifications of 60%, 90%, 98% and the like are finally obtained, and the method is simple, convenient, feasible and suitable for industrialization.