Method for detecting medicine for treating diabetes mellitus

A diabetes drug and detection method technology, applied in the field of biomedicine, can solve problems such as thin-layer detection of untreated diabetes drugs, and achieve a significant effect of curative effect

Inactive Publication Date: 2018-06-29
JINAN HAOYU QINGTIAN PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At home and abroad, there is no drug that completely cures diabetes. So far, there have been no reports or patents related to the treatment of diabetes with extracts from neem leaves and Chinese toon leaves. Related reports

Method used

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  • Method for detecting medicine for treating diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of medicine for treating diabetes

[0035] (1) Take neem leaves and Chinese toon leaves of the same weight, mix them evenly, crush them, pass through a 20-mesh sieve, use petroleum ether as a solvent, extract twice at 50°C, each extraction time is 3 hours, and the amount of solvent used each time is neem leaves and 12 times of the total weight of Toona sinensis leaves, filtered, combined petroleum ether extracts, reclaimed petroleum ether under reduced pressure, concentrated and dried to obtain crude extract A, and the dregs after petroleum ether extraction are for subsequent use;

[0036] (2) Extract the dregs after petroleum ether extraction in step (1) with ethyl acetate-methanol mixed solvent with a volume ratio of 1:6, and extract three times at 70°C, each extraction time is 2 hours, and the amount of solvent used each time is 10 times the total weight of neem leaves and Chinese toon leaves, filter, combine the ethyl acetate-methanol e...

Embodiment 2

[0042] Embodiment 2: content determination.

[0043] High performance liquid chromatography-evaporative light scattering method was used to analyze the content of components. Using YMC-Pack C 18 (150mm × 4.6 mm, 5 μm) chromatographic column, eluting with mobile phase methanol-water-tetrahydrofuran (50:44:6), flow rate 1.0mL / min, column temperature 25°C; using evaporative light scattering detector , the drift tube temperature is 92.0°C, and the carrier gas flow rate is 2.2L / min. The results showed that the drug for treating diabetes in Example 1 contained azalea C, azalea F, and azadiractone K, and the three components accounted for 66% of the total weight of the drug for treating diabetes.

[0044] Table 1 Components and contents of limonoids in the extract

[0045]

Embodiment 3

[0046] Embodiment 3: Composition structure identification

[0047] The drug for treating diabetes was prepared according to the method in Example 1, and then subjected to 200-300 mesh silica gel column chromatography, firstly eluted with petroleum ether-ethyl acetate gradient (10:1-1:1), and then ethyl acetate-methanol ( 10:0.5-2:1) Gradient elution thin-layer tracking detection, combined the same fractions, static crystallization, suction filtration, ethyl acetate or acetone recrystallization, respectively to obtain compound 1, compound 2, compound 3, both White amorphous powder, the chemical structures of the above compounds are confirmed by mass spectrometry and nuclear magnetic resonance as: aphanamixoid C (aphanamixoid C), aphanamixoid F (aphanamixoid F), chukvelutilide K , and the purity was greater than 98% as detected by high performance liquid chromatography.

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Abstract

The technical scheme discloses a method for detecting a medicine for treating diabetes mellitus. A method for preparing the medicine for treating the diabetes mellitus comprises the following steps: extracting, separating and purifying raw materials so as to obtain the medicine, containing aphanamixoid C, aphanamixoid F and chukvelutilide K, for treating the diabetes mellitus, wherein meliaceae leaves and cedrela sinensis leaves are taken as raw materials; according to the detection method, when a silica gel G thin layer plate is adopted for detecting, three characteristic spots can be seen; when high performance liquid chromatography (HPLC) is adopted for detecting, three characteristic peaks can be seen.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a detection method for a drug for treating diabetes, in particular to a method for detecting a drug for treating diabetes prepared by using neem leaves and Chinese toon leaves as raw materials. Background technique [0002] Diabetes mellitus is a metabolic disease caused by insufficient insulin secretion or insensitivity of peripheral tissues to insulin. etc.) long-term impairment, insufficiency or failure. According to different pathogenesis, diabetes can be divided into type Ⅰ diabetes, type Ⅱ diabetes, gestational diabetes and other types of diabetes. [0003] According to statistics, in 2015, about 415 million people aged 20-79 in the world suffered from diabetes (8.8% prevalence rate), and another 318 million people had impaired glucose tolerance (pre-prevalence rate was 6.7%). China is the country with the largest number of diabetic patients in the world. In 2015, the number of p...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/90A61K36/58A61P3/10A61K127/00
CPCA61K36/58A61K2236/331A61K2236/333A61K2236/35A61K2236/39A61K2236/55G01N30/02G01N30/06G01N30/90A61K2300/00
Inventor 不公告发明人
Owner JINAN HAOYU QINGTIAN PHARMA TECH CO LTD
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