The invention belongs to the technical field of
molecular biology detection. Specifically, the application provides a
magnetic bead method
saliva DNA extraction kit that only needs one-step washing, which includes a
lysis solution, a binding solution, a
proteinase K solution, a washing solution and an
elution solution. buffer. A
saliva DNA extraction kit includes lysing solution, binding solution,
proteinase K solution, washing solution and
elution buffer. The lysate contains
Tris, EDTA, NaCl, NaDC, dodecyl-N-
betaine, NLS, GuHCl, TritonX100, NH 4 Cl, mercaptoethanol; binding solution including isopropanol, PEG 8000, Brij 58; washing solution including GuHCl,
Tris,
ethanol,
sodium bicarbonate;
elution buffer including
Tris, EDTA. In the present application, by preparing a washing solution containing a relatively
high concentration of
sodium bicarbonate (slightly stronger than
sodium acetate and sodium
chloride), the
ion balance and the effect of providing
alkalinity are taken into account at the same time, and a zwitterionic detergent is used in combination.
Alkyl-N-
betaine, a
magnetic bead-based
saliva DNA extraction kit was constructed that only required one-step washing.