The invention discloses a kit for semi-quantitative allergen screening. The kit comprises a nitrocellulose membrane strip coated with 19 allergens, a biotinylated anti-human IgE antibody, streptavidin labeled by alkaline phosphatase and a substrate solution, the allergens include willow / poplar / elm, common ragweed, mugwort, dermatophagoides pteronyssinus / dermatophagoides farinae, house dust, cat hair, dog epithelium, cockroaches, penicillium notatum / cladosporium / aspergillus fumigatus / alternaria, scandent hop, egg white, milk, peanuts, soybeans, beef, mutton, cod / lobster / scallop, shrimps and crabs. 19 common allergens can be qualitatively and quantitatively detected through one-time detection, and the method has the advantages that the operation is simple, the detection efficiency is greatly improved, the production and use cost is reduced, and the popularization and application of allergen detection are facilitated.