Method for inducing mouse pruritus cutanea model using dermatophagoides pteronyssinus protein and SEB

A technology for skin pruritus and house dust mite, which is applied in the field of animal experimental model construction, can solve the problems of high price, inconvenient operation, and restrictions on wide application, and achieve the effect of deep modeling severity and short modeling cycle

Inactive Publication Date: 2021-10-08
江西中洪博元生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Spontaneous animal models include Nc/Nga mice, which are characterized by severe erythema, itching, dry skin, skin barrier dysfunction and other symptoms in ordinary environments, and the pathological manifestations are close

Method used

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  • Method for inducing mouse pruritus cutanea model using dermatophagoides pteronyssinus protein and SEB
  • Method for inducing mouse pruritus cutanea model using dermatophagoides pteronyssinus protein and SEB
  • Method for inducing mouse pruritus cutanea model using dermatophagoides pteronyssinus protein and SEB

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Experimental program
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Effect test

Embodiment

[0039] 1. Experiment preparation

[0040] Animals: 8w-old C57B / 6N mice, male or female.

[0041] Reagents and consumables: house dust mite extract (lot: 361863, GREER), SEB (lot: 92815B, Toxin Technology), PBS (lot: M61218, TRANSGEN BIOTECH), cotton pads, adhesive tape.

[0042] Preparation of house dust mite protein solution: Take a bottle of house dust mite extract (containing 4.51 mg of protein), add 4.51 ml of PBS to completely dissolve the dust mite, prepare a 1 mg / ml mother solution, and dilute it with PBS to 100 μg / ml each time. ml of house dust mite protein (Derf) solution.

[0043] Preparation of SEB: Take a bottle of SEB, add 10ml of PBS to prepare a 100μg / ml mother solution, and dilute it with PBS to 10μg / ml of SEB each time.

[0044] Before the following modeling operations, the mice were adaptively fed for 7 days.

[0045] 2. Modeling operation method

[0046] 1. After isoflurane anesthesia, shave the hair on the back, then apply the depilatory cream, 10 minut...

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Abstract

The invention provides a method for inducing a mouse pruritus cutanea model by using dermatophagoides pteronyssinus protein and SEB, which comprises the following steps: adaptively feeding a mouse for 7 days, anesthetizing the mouse with isoflurane, shaving the back, smearing depilatory paste, scraping the depilatory paste and hair after 10 minutes, forming a 3-5cm < 2 > depilatory area, slightly scraping the depilated skin to cause skin lesion, and the like. According to the method, a mixed allergen is adopted to sensitize the local skin of the mouse, the total time of model preparation and model preliminary verification is 40 days, the model making period is shorter than that of 7 weeks of sensitization of a single allergen, and the model making severity is deeper. The mouse strain is common C57BL/6 mouse, the price is low, and the model forming rate is 70% or above.

Description

technical field [0001] The invention belongs to the technical field of animal experiment model construction and relates to a method for inducing a mouse skin pruritus model by using house dust mite protein and SEB. Background technique [0002] Pruritus (atopic dermatitis) is a chronic skin disease without primary skin lesions. It can be stimulated by certain diseases, drugs, cold, and allergens. It is a neurological dysfunction skin disease, and it is still a refractory skin disease. Clinically, the patient feels severe itching without primary skin lesions. The site of occurrence is not fixed and can be generalized or limited to certain parts such as: head and neck, trunk, limbs, and vagina. Scratch marks, papules, and blood scabs can occur after itching , skin thickening and lichenification changes, the patient is itching unbearably, suffering unbearably. [0003] The current common model animals for atopic dermatitis include rabbits, rats, mice, guinea pigs, dogs, etc. ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2207/10A01K2207/30A01K2267/03
Inventor 江斌李焱袁永明
Owner 江西中洪博元生物技术有限公司
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