103 results about "Corydalis saxicola" patented technology

The invention discloses an application of protoberberine alkaloid to prepare anti-drug tolerant bacterial drug, in particular to an application of compound coptisine, dehydrokaweidin and dihydroapokaweidin in the anti-drug tolerant bacterial drug, which is characterized by the following: extracting protoberberine from Corydalis saxicola Bunt. of Corydalis of Papaveraceae; displaying obvious inhibition for MRSA in the clinic through pharmacological test; using the compound protoberberine alkaloid as active component to make the drug to treat anti-drug tolerant bacterial drug.

The invention relates to a breeding method for backup boars and sows. The method includes the following steps of giving following feed including 400-600 parts of pennisetum hydridum, 600-700 parts of maize, 50-100 parts of wheat bran, 200-300 parts of bean pulp, 20-50 parts of fish meal, 0.1-0.3 part of vitamin, 1-2 parts of kudzuvine root extract, 1-2 parts of rhizome drynariae extract, 1-2 parts of morinda officinalis extract, 0.1-0.2 part of genistein, 1-4 parts of fructose and 1-5 parts of glucose; making backup boars and sows freely eat the feed; regularly giving extract each year for a week each time, wherein the extract is prepared from, by weight, 5-20 parts of poria cocos, 5-10 parts of astragalus membranaceus, 1-3 parts of fennel and 1-5 parts of corydalis saxicola bunting. By means of the method, health of backup boars and sows can be effectively improved, the estrus rate is increased, and the breeding success rate of backup boars and sows is increased.

The invention relates to the natural medicine field, in particular to a method for preparing total alkaloids of corydalis saxicola. Based on the total alkaloids crude extracts of corydalis saxicola and after repeated acid dissolving and alkali precipitating, the invention can make total alkaloids extracts of corydalis saxicola, the content of which is higher than 50 percent.

The invention provides a standardizing planting method of corydalis saxicola, belongs to the technical field of agriculture, and particularly relates to a planting method of corydalis saxicola. The standardizing planting method of corydalis saxicola has the advantages that high-quality plants are domesticated, fertilizer is applied with a nutritional prescription, nuisanceless pest and insect control is realized, the production management techniques and medicines meet standards, and the output is high. The method comprises the following steps of (1) land selection and arrangement: selecting the land with wet and shielding conditions, wherein the soil is favorably loam or sand soil with loose soil texture and good water retaining capability; (2) culturing of seedlings: after the seeds in May are matured, immediately burying by land, and storing; (3) planting: culturing in exposed land in later autumn, winter and spring with lower temperature and less heavy rain, so as to avoid the injury to corydalis saxicola plants by high-temperature and heavy rain seasons of summer and early autumn; (4) topdressing: using organic fertilizer, and combining with inorganic fertilizer; (5) harvesting and processing in place of origin: harvesting the artificially cultivated corydalis saxicola after blossoming period in May, digging the whole plant with root, cleaning to remove soil, airing, and storing at air-ventilated and dry places.

The invention provides corydalis saxicola tea capable of clearing away heat and toxic materials and a preparation method thereof. The tea is prepared from tea and a Chinese herbal medicine extract in a mass ratio of 1 to 4, wherein the Chinese herbal medicine extract comprises the following raw materials by weight percent: 20-40% of corydalis saxicola, 10-20% of all-grass of Japanese violet, 10-15% of herb of rupestrine dragonhead, 10-15% of bark of Chinese white poplar, 5-10% of betula ermanii, 5-10% of herb of twinflower groundsel, 5-10% of all grass or root of common calanthe, 3-5% of herb of bigflower clinopodium, 3-5% of barbed skullcap herb and 3-5% of leaf of common dysosma. The preparation method comprises the following main steps: a. material preparation; b. filtrate extraction; c. centrifugal separation and concentration; d. smearing and drying; e. parching and sterilizing. The corydalis saxicola tea capable of clearing away heat and toxic materials has the effects of clearing away heat and toxic materials, relieving itching and promoting diuresis as well as stopping pain and bleeding after being drunk for a long term.

The invention discloses lucid ganoderma-corydalis saxicola compound chewable tablets. The formula of the lucid ganoderma-corydalis saxicola compound chewable tablets comprises 10-25 parts of lucid ganoderma, 10-15 parts of fructus lycii, 2-6 parts of corydalis saxicola, 5-10 parts of fructus momordicae, 20-30 parts of hydroxypropyl methyl cellulose, 5-15 parts of maltodextrin and 1-3 parts of lubricant. A production method of the lucid ganoderma-corydalis saxicola compound chewable tablets comprises the following steps: grinding the raw materials separately and sieving with a 500-mesh sieve to obtain ultrafine powder; combining according to the formula and stirring uniformly; granulating with alcohol by a wet process; drying at 60-80 DEG C until the water content reaches 4-6% by weight; cooling to room temperature, and sieving with a 60-mesh sieve; adding the lubricant and tabletting to obtain the lucid ganoderma-corydalis saxicola compound chewable tablets. The chewable tablets disclosed by the invention have the healthcare effects of clearing heat and removing toxin, protecting and nourishing liver, adjusting immunity, reducing blood sugar and blood fat, resisting oxidation and aging, replenishing vital essence to improve eyesight, promoting longevity and the like.

The invention provides a method for extracting and preparing corydalis saxicola bunting total alkaloid, which is applied to the extraction of the corydalis saxicola bunting total alkaloid by taking corydalis saxicola bunting plants as raw materials. The method has the advantages of effectively combining unit operations of acid water extraction, salting out and active carbon decoloring and degreasing, etc.; moreover, products obtained by the method have the advantages of stable and uniform color and luster, high content of effective components and good melting property, and corydalis saxicola bunting injections prepared by the materials obtained by the method have the advantages of good clarity, high extraction yield of the corydalis saxicola bunting total alkaloid and low manufacturing cost.

The invention provides a tissue culture and rapid propagation method for corydalis saxicola bunting. The tissue culture and rapid propagation method comprises the following steps: (1) taking a corydalis saxicola bunting lateral bud as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain cluster buds; (3) putting the cluster buds into an MS strong culture medium to be subjected to strong seedling culture to obtain a strong plant; (4) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (5) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one and a half months and then transplanting the seedling to a large field. With the adoption of the culture method, an obtained germchit is strong and the survival rate is high; a lot of high-quality germchits of the corydalis saxicola bunting can be provided in short time so that the large-scale seedling growth problem of the corydalis saxicola bunting is solved effectively.

The invention relates to a raising method for producing sows. The raising method includes the following steps: feeding feed composed of Pennisetum sinese roxb, corn, egg powder, wheat bran, soybean meal, fish meal, meat and bone meal, 20-30 parts of linseed meal, soybean oil, histidine, arginine, sucrose powder and vitamin, wherein a feeding method includes: feeding 3-4kg for each sow each day before delivery, feeding 1-2kg for each sow at the day of delivery, gradiently adding 0.5kg for each sow each of four days after delivery, and allowing the sows to ingest freely after 3-5 days; regularly feeding extract prepared with poria cocos, astragalus, aniseed and Corydalis saxicola each mouth, wherein feeding interval is a week. By the raising method, physique of the producing sows can be enhanced effectively, productivity of the producing sows can be improved, and nutrition yield and quality of breast milk can be improved.

The present invention relates to a kind of freeze-dried powder injection for curing hepatitis, its medicine composition for fluid infusion and their preparation method. The freeze-dried powder injection composition contains 1 portion of effective component extracted from root of meadowrueleaf corydalis and 50-500 portions of auxiliary material, and the medicine composition for fluid infusion contains 1 portion of effective component of root of meadowrueleaf corydalis and 800-1000 portions of auxiliary material, and their preparation method includes the following steps: decocting root of meadowrueleaf corydalis, separating and purifying to obtain its extract, mixing it with auxiliary material uniformly, dissolving them in injection water, filtering, filling and sterilizing so as to obtain the invented product.

The invention relates to a feeding method for suckling piglets. The method comprises the following steps of 1 sow lactagogue feeding, wherein a lactating sow is fed with feed for promoting milk secretion; 2 piglet feeding, wherein a hog house is kept at the constant temperature of 30 DEG C, nipple fixing for the piglets is helped through manpower, nipple fixing is achieved by taking natural selection of the piglets as a main mode and taking individual control as an auxiliary mode, the small and weak piglets are helped suck the breasts while milk robbing of the strong piglets is controlled, and meanwhile the piglets are supplemented with plant material extracting solutions which are prepared from, by weight, 5-20 parts of poria cocos, 1-5 parts of corydalis saxicola, 5-10 parts of astragalus membranaceus and 1-3 parts of foeniculum vulgare through intranasal administration in the first day to the twentieth day after the piglets are born. According to the feeding method for the suckling piglets, the breast milk quality of the sow is improved from sow feeding, the plant materials with the healthcare function is fed to the piglets to enhance the immunity of the piglets, reasonable manual intervention is additionally performed, therefore, it is guaranteed that the piglets can grow healthily in the suckling period, and the survival rate of the piglets is greatly increased.

The invention discloses a traditional Chinese medicine composition for treating non-alcoholic fatty liver disease. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: corydalis saxicola, rhizoma picrorhizae, pseudo-ginseng, radix salviae miltiorrhizae, kudzuvine root, rhizoma atractylodis macrocephalae, radix paeoniae alba, tuckahoe, radix bupleuri, angelica, lotus leaves, semen coicis, chinese waxgourd semen, reed rhizome, platycodon grandiflorus, semen lepidii, loquat leaves, fritillaria cirrhosa, fructus lycii, semen cassia, rhizoma alismatis, peach kernel and mulberry leaves. Pharmacological experiments prove that the traditional Chinese medicine composition is capable of preventing insulin resistance, improving insulin sensitivity, reducing blood fat, inhibiting inflammatory reaction, improving abnormal glucolipid metabolism, reducing hepatocyte fat deposition, improving liver function, decreasing liver indexes and having obvious treatment effects on a rat suffering from non-alcoholic fatty liver disease.

The invention discloses a paraffin slicing method for corydalis saxicola blades. The method comprises the following steps: fixing a sample, dehydrating, transparently processing, waxing, embedding, slicing and unfolding slices, de-waxing, dyeing and sealing slices. The dyeing step further comprises the following steps: before transparently processing, putting the dehydrated corydalis saxicola blades into a safranine solution in volume concentration of 0.8-1.2%, and dip-dyeing for 8-12h; rehydrating a slide carrying the corydalis saxicola blades in the dyeing step; after rehydrating, putting into an alcoholic solution for separating colors; adding acetic acid at the volume ratio of 1 / (800-1000) into the alcoholic solution used for separating colors; and finally, putting into a fast green solution in volume concentration of 0.8-1.2%, and dyeing for 5-15 seconds. The corydalis saxicola blades prepared according to the invention are clear in outline tissues and are suitable for identification of medicinal materials.

The invention discloses a preparation method of an antibacterial carbon fiber filter screen. The preparation method comprises the following operating steps of S1, compounding antibacterial liquid: adding small carpesium abrotanoides fruits in a composite family, lindley butterflybush flowers, herbal corydalis saxicola and emilia sonchifolia to boiling water, continuing decocting by a small fire for 2-3 hours, performing filtering to obtain filtrate, then adding xanthan gum to the obtained filtrate, and performing uniform mixing and stirring so as to obtain the antibacterial liquid; S2, modifying a carbon fiber filter screen: placing the carbon fiber filter screen in mixing liquid of 85-90 DEG C, and performing soaking treatment for 50-60 min; and S3, performing padding: padding the modified carbon fiber filter screen by the antibacterial liquid, wherein the padding pressure is 1-3kg / cm<2>, and after performing padding, performing drying treatment so as to obtain a finished product. The activated carbon fiber filter screen prepared through the adoption of the preparation method disclosed by the invention is notable in filtering effect, and notable in adsorbing effect of an ash layer; and the situation that the ash layer enters indoor air again can be effectively avoided, the restraining effect of pathogenic bacteria is notable, and viruses and microorganisms adsorbed on the surface of the filter screen can be effectively killed.

The invention relates to a wintergreen barberry root suppository and a sustained and controlled release wintergreen barberry root suppository, belonging to the technical field of pharmaceuticals. The preparation is prepared by the following steps: using wintergreen barberry roots as raw materials and carrying out steps of pulverization, reflux extraction, decompression concentration, pH regulation, and the like to obtain a tan wintergreen barberry root extract; using the tan wintergreen barberry root extract as an active pharmaceutical ingredient and adding a fat-soluble suppository matrix orsustained and controlled release macromolecular materials to prepare the wintergreen barberry root suppository or the sustained and controlled release wintergreen barberry root suppository. The preparation has obvious anti-inflammatory effect and can be prepared into a rectal suppository used for treating internal hemorrhoids and rectal inflammations and a vaginal suppository used for treating vaginitis and acute vaginitis according to different application and administration routes.

The invention discloses a method for promoting germination of corydalis saxicola bunting seeds. According to the method, wild corydalis saxicola bunting seeds which are harvested in May to June every year are wet-stored for 2-6 weeks at low temperature before being cultured, so that the corydalis saxicola bunting seeds are effectively awakened from the dormant state to promote germination; by controlling the culture temperature to be low temperature about 15 DEG C and by performing illumination and shielding culture alternatively, a natural growing environment of corydalis saxicola bunting is effectively simulated, and the germination rate of the corydalis saxicola bunting seeds is increased. In addition, by taking a nutrient solution containing micron-order limestone grains which are obtained from growing places of the wild corydalis saxicola bunting and multiple inorganic salts as a culture medium, the germination rate is increased. By adopting the method for promoting the germination of the corydalis saxicola bunting seeds, the germination rate of the corydalis saxicola bunting seeds is increased to be over 35 percent.

The invention provides a corydalis saxicola bunting imitated wild cultivation method which comprises the following specific steps of preparing a culture medium which takes turf soil, fungus dregs, cattle manure and the like as main raw materials; then punching a plurality of cultivation holes in a rock wall; filling and cultivating a medium in each cultivation hole, then putting seeds into the cultivation holes, and spraying water to keep the humidity of each cultivation hole; after corydalis saxicola bunting seedlings grow to 3-5cm, enabling the corydalis saxicola bunting seedlings to grow naturally till harvesting. By virtue of implementing the method, corydalis saxicola bunting grows in a steep imitated wild environment, so that the natural property and high medical effect of medicinal herbs can be ensured, the harvesting time is flexible to master, the farming season is not occupied, farmland is not occupied, pesticides, fertilizers and hormone are not used in a growing process, no disease and insect damage is generated, and the corydalis saxicola bunting completely grows naturally; the situation that corydalis saxicola bunting medicinal herbs are nearly extinct due to coyoting over the year is avoided; not only can wild resources be protected, but also scale development is realized, and obvious economic benefit and social benefits are achieved.

The invention discloses a paraffin sectioning method for Corydalis saxicola Bunting stems. The paraffin sectioning method comprises the following steps: sample fixation, dehydration, transparency treatment, wax dipping, embedding, sectioning and slice expansion, dewaxing, dyeing and slice sealing. The paraffin sectioning method specifically comprises the following steps: before the transparency treatment, putting dehydrated Corydalis saxicola Bunting stems into a 0.8%-1.2% sarranine solution, and carrying out dip dyeing for 8-12 hours; after the dewaxing, carrying out rehydration on a slide carrying the Corydalis saxicola Bunting stems, putting the Corydalis saxicola Bunting stems into an alcohol solution, carrying out color separation, simultaneously adding acetic acid into the alcohol solution for the color separation in a volume ratio of 1 to (800-1000), finally, putting the Corydalis saxicola Bunting stems into a 0.8%-1.2% fast green solution, and carrying out dyeing for 10-20 seconds. By virtue of the paraffin sectioning method disclosed by the invention, a clear Corydalis saxicola Bunting stem structure can be sectioned, a specimen can be stored for a long time for use, is a permanent microscopic slide specimen and can be applied to microscopic identification of Corydalis saxicola Bunting medicinal materials for market normalization.

A method for preparing a composition of five alkaloids of Litectin Rhizoma, comprising: (1) taking 1 part of crude extract of total alkaloids of Litectin Rhizoma Litectinus, refluxing and dissolving with 10-100 parts (V / W) of 75-100% ethanol solution , add 0.5-5 parts (W / W) of silica gel to mix the sample, stir and evaporate to dryness to obtain the mixed sample silica gel; (2) soak 6-20 parts (W / W) of silica gel in 5:1-1:1 ( V / V) of ethyl acetate and ethanol was applied to the column by wet method, and the mixed sample silica gel obtained in step (1) was added, and the gradient elution was performed with 3:1-1:1 (V / V) ethyl acetate and ethanol , collecting 1 / 1 of the eluate, concentrating and filtering to collect the filter cake, and drying to obtain the five alkaloid compositions of Litectin. The preparation method of the invention has the advantages of simple equipment, low cost, easy operation, short required time, high output and high safety.

The invention provides a wild-imitating cultivation method for corydalis saxicola in a cavern, and belongs to the technical field of agriculture. The invention provides the wild-imitating cultivationmethod for the corydalis saxicola in the cavern for maintaining quality of medicinal materials in a wild original shape, and having low costs, a high survival rate, strong pest and disease resistanceand a high yield. The method comprises the following steps: 1, selecting the cavern: selecting the cavern with a wide opening, sufficient scattering illumination in the cavern and moist soil to cultivate the corydalis saxicola; 2, preparing soil and applying a base fertilizer: removing ground weeds, shrubs and stone riprap in the cavern, and requiring stepping surface soil to be finely smashed andsmooth when soil is prepared; 3, enabling a planting manner to be mainly based on an original direct sowing manner; 4, treating seeds and performing sowing; 5, performing field management; 6, preventing and controlling diseases; and 7, performing harvesting.

The invention discloses a preparation method of a traditional Chinese medicine for treating constipation-predominant reflux esophagitis and belongs to the technical field of preparation methods of traditional Chinese medicines. At present, bismuth potassium citrate is usually employed for treating the constipation-predominant reflux esophagitis, and the contraindication is that allergies, patients with severe renal impairment and pregnant patients are disabled. The technical scheme of the preparation method comprises: taking senna leaf, cape jasmine fruit, pagodatree pod, garden burnet root, Chinese arborvitae twig, immature bitter orange, officinal magnolia bark, longstamen onion bulb, areca peel, peach seed, Chinese angelica, figwort root, black sesame, hemp fruit, Chinese dwarf cherry seed, honey, apricot seed, coffee senna seed, common alstonia bark or leaf, common lophatherum herb, mung bean, mongolian dandelion herb, stonecrop, shortstalk slimtop meadowrue root, breynia fruticosa, small-leaved desmodium, physalis alkekengi, corydalis saxicola bunting, uniflower swisscentaury root, olive, lemmaphyllum microphyllum presl, creeping rostellularia herb, gamboges, potentilla discolor, rhubarb and liquoric root; soaking the thirty six traditional Chinese medicines into water, and decocting by mild fire, wherein the decocted soup is the traditional Chinese medicine for treating the constipation-predominant reflux esophagitis. The preparation method of the traditional Chinese medicine for treating the constipation-predominant reflux esophagitis has the advantages that the prepared traditional Chinese medicine liquid is small in side effect, short in course of treatment and high in cure rate.

The present invention discloses an application of corydalis saxicola and a preparation thereof in treating non-alcoholic fatty liver diseases and / or non-alcoholic steatohepatitis. According to the invention, through a large amount of experimental screening, it is found that corydalis saxicola total alkaloids have good effects of reducing synthesis in fatty acid cells, and can reduce the levels ofTG, TC, LDL cholesterol, HDL cholesterol and free fatty acid induced by HFD. Animal anatomical experiments show that the corydalis saxicola total alkaloids can effectively reduce the weight of livers,and pathological sections show that liver tissues are well protected. In addition clinical experiments show that corydalis saxicola total alkaloid capsules can obviously improve biochemical indexes and imaging evaluation results of fatty liver patients. The drug-induced liver injury experimental results show that the corydalis saxicola total alkali capsules can obviously improve drug-induced liver injury and have the effect of preventing liver injury.

The invention provides a production method of high purity Corydalis saxicola Bunting alkaloid. The method mainly includes the steps of: 1) drying and crushing medicinal materials; 2) conducting enzymolysis on the compound enzyme composed of cellulose and pectinase; 3) using ethanol with a volume concentration of 50-90% as the extraction solvent to conduct extraction concentration; 4) carrying out centrifugal separation; 5) performing treatment with centrifugal separation to obtain crude total alkaloid; and 6) subjecting the crude total alkaloid to two-stage membrane filtration, thus obtaining a purified product. The method provided by the invention has the characteristics of simple and practicable operation, mild extraction process conditions, high extraction rate, small alkaloid loss in the separation and purification process, high recovery rate, and high product purity, and can obtain Corydalis saxicola Bunting alkaloid with a total alkaloid content of greater than 98%, thus completely meeting pharmacy requirement. Through detection of the product, the Corydalis saxicola Bunting alkaloid content is 98.6%, and the heavy metals, pesticide residues and microorganism are all accord with the national standard GB / T5009, 4789 standards.

The invention discloses liver protecting medicinal liquor. The liver protecting medicinal liquor is formed by brewing raw materials which include, by weight ratio, 10-100 of corydalis saxicola bunting, 1-15 of schisandra chinensis, 1-20 of liquorice, 1-15 of red-rooted salvia roots, 5-20 of lucid ganoderma, 5-20 of fructus lycii, 20-100 of rock candy and 1000 of white spirits, wherein all the raw materials except the rock candy and the white spirits are dried, and the alcoholic strength of the white spirits ranges from 20 V / V% to 60 V / V%. All components of the liver protecting medicinal liquor are high in compatibility and cooperativity, the effect of protecting livers is achieved on different aspects, through the characteristic that the white spirits achieve drug potentials, various types of medicine materials are promoted to rapidly achieve effects, the burdens on livers can be reduced, nutrients are increased for livers, blood circulation of livers is improved, livers are protected, and the effect of assisting in treating liver diseases and liver damage is achieved.

The invention discloses application of a meadowrue-corydalis-root traditional Chinese medicine composition to prepare medicaments treating heart disease, that is, the invention discloses a new purpose of a traditional Chinese medicine composition employing meadowrue corydalis root and moschus as raw materials to prepare medicaments treating and / or preventing heart disease.

The invention discloses a high-efficiency induction method of Corydalis saxicola Bunting calluses. The method comprises the following steps: taking Corydalis saxicola Bunting explant, washing and disinfecting the Corydalis saxicola Bunting explant, inoculating an induction medium with the disinfected Corydalis saxicola Bunting explant, and carrying out induction culture for 10-15 d to obtain calluses, wherein the induction medium contains a B5 medium as a basal medium, and also contains 0.5-1.5 mg / L of 6-benzyladenine, 0.5-1.5 mg / L of 2,4-dichlorophenoxyacetic acid, 5-20 mg / L of vitamin C, 0.1-0.3 g / L of active carbon, 20-30 g / L of sucrose and 3.8-4.5 g / L of agar, and the pH value of the induction medium is 6.0. The method can be used to highly-efficiently induce the calluses and make the calluses rapidly propagate in order to solve the problem of difficulty in large-area breeding of wild Corydalis saxicola Bunting.

The invention discloses a traditional Chinese medicine composition for treating severe infantile pneumonia and a preparation method thereof. The traditional Chinese medicine composition disclosed by the invention contains the main components including corydalis saxicola bunting, musk, peristrophe and wedelia prostrate and is reasonable in composition, scientific in process and convenient for operation; the traditional Chinese medicine composition has obvious treatment effect for severe infantile pneumonia and has the characteristics of being rapid in effect, good in treatment effect, free from toxic and side effects and the like; compared with the prior art, significant breakthrough is made; the traditional Chinese medicine composition has outstanding practical characteristics and significant progress.

The invention discloses a Chinese medicine composition for diminishing inflammation and relieving sore throat and a preparation method thereof. The Chinese medicine composition is prepared from the raw materials of spreading hedyotis herb, common andrographis herb, screwtree root, corydalis saxicola bunting, Japanese peristrophe herb and Japanese polygala. The invention further provides a preparation method of the Chinese medicine composition for diminishing inflammation and relieving sore throat. The composition disclosed by the invention has the functions of clearing away heat and toxic materials, diminishing inflammation and removing stasis, and is accurate in curative effect, good in safety and free from side effects on the aspect of treating acute and chronic pharyngolaryngitis, acute tonsillitis and the like.

The invention discloses an in-vitro preservation method of corydalis thalictrifolia. The method comprises the following steps: performing tissue culture two-step seedling development on corydalis thalictrifolia, and inoculating test-tube seedlings with 4-6 leaves obtained by utilizing tissue culture two-step seedling development into a preservation medium for performing in-vitro preservation. The preservation medium refers to 1 / 2MS+PPP3331.0mg / l+0.3% of AC+ 4.5% of cane sugar+0.4 % of agar. The pH value of the medium is 5.8, the temperature is 21+ / -1 DEG C, the illumination intensity is 1400-1600lx, and the illumination time is 8-10 hours per day. According to the method disclosed by the invention, lots of high quality seedlings of corydalis thalictrifolia can be provided in a short time, the steps of preparing the medium and spinning the bottle are reduced, and lots of manpower and material resources are saved; and therefore, the method is simple and feasible, the production cost is reduced, and the purpose of preservation and large-scale seedling growing of corydalis thalictrifolia germplasm resources can be effectively achieved.