In-vitro preservation method of corydalis thalictrifolia

A technology of in vitro preservation and coptis chinensis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult survival, wild coptis chinensis resources are on the verge of depletion, and difficulty in germplasm preservation and utilization, and achieves a high survival rate. , Solve the problem of germplasm resource conservation and large-scale seedling breeding, and the effect of robust seedlings

Inactive Publication Date: 2016-07-13
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology

In recent years, people have been digging and purchasing a lot, which has led to the shortage of wild resources, and the resources of wild rock berberia are on the verge o

Method used

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  • In-vitro preservation method of corydalis thalictrifolia
  • In-vitro preservation method of corydalis thalictrifolia

Examples

Experimental program
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Embodiment Construction

[0018] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0019] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0020] A method for in vitro preservation of the genus Litectin, realized by the following methods:

[0021] 1. Cultures obtained from rapid propagation of Litectin:

[0022] 1. Test materials

[0023] Yan Huanglian Corydalissaxicola Bunting shoot tip.

[0024] 2. Disinfection of explants

[0025] Soak the selected stem tips in 75% ethanol for 30-45s, rinse them with sterile water for 2-3 times, put them into 0.1% mercuric chloride with 2% Tween-20 for 4min, and rinse them with sterile water for 2-3 times , put into 0.1% mercuric chloride added with 2% Tween-20 again for 4 minutes, and finally rinse with sterile water 2 to 3 times.

[0026] 3. Explant induction

[0027] Sterilize the collected shoot tip of Litectinus chine...

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Abstract

The invention discloses an in-vitro preservation method of corydalis thalictrifolia. The method comprises the following steps: performing tissue culture two-step seedling development on corydalis thalictrifolia, and inoculating test-tube seedlings with 4-6 leaves obtained by utilizing tissue culture two-step seedling development into a preservation medium for performing in-vitro preservation. The preservation medium refers to 1/2MS+PPP3331.0mg/l+0.3% of AC+ 4.5% of cane sugar+0.4 % of agar. The pH value of the medium is 5.8, the temperature is 21+/-1 DEG C, the illumination intensity is 1400-1600lx, and the illumination time is 8-10 hours per day. According to the method disclosed by the invention, lots of high quality seedlings of corydalis thalictrifolia can be provided in a short time, the steps of preparing the medium and spinning the bottle are reduced, and lots of manpower and material resources are saved; and therefore, the method is simple and feasible, the production cost is reduced, and the purpose of preservation and large-scale seedling growing of corydalis thalictrifolia germplasm resources can be effectively achieved.

Description

technical field [0001] The invention belongs to the technical field of biological tissue culture, and in particular relates to an in vitro preservation method of Litectin chinensis. Background technique [0002] Corydalissaxicola Bunting (Corydalissaxicola Bunting) is a plant of the Papaveraceae family, a perennial herb, distributed in Guangxi, Guangdong, Fujian, Hunan and other provinces in my country. The whole herb is used as medicine, and it is a famous Zhuang Yao medicine variety. The whole herb contains active ingredients such as dehydrocarvitin (petidine); it has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been observed to inhibit tumor cells. Function; Indications for treating sores, furuncles, swollen toxins, hepatitis, cirrhosis, and liver cancer. Due to the poor habitat conditions, its natural reproduction rate is very low, and its seeds are small, so it is difficult to reproduce, the population development i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008
Inventor 张占江李翠郭晓云赵以民李林轩缪剑华韦莹韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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