Method of preparing five-alkaloid composition of corydalis saxicola bunting
The technology of alkaloid and composition is applied in the field of preparation of five kinds of alkaloid compositions of Coptidis chinensis, which can solve the problems of low total alkali content and poor water solubility, and achieve the effects of simple equipment, reduced toxic and side effects, and low cost.
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Embodiment 1
[0024] Take 2.0g of the crude extract of total alkaloids of Litectinus chinensis, add 200ml of 75% ethanol, heat to reflux for 1 hour, filter while it is hot, add 2.0g of silica gel to the filtrate, stir and evaporate to dryness, and then get the mixed sample silica gel; take 12g of silica gel with ethyl acetate After soaking in ester: ethanol (3:1), put it on the column by wet method, fill the sample silica gel on the top of the column, carry out gradient elution with ethyl acetate: ethanol (3:1-1:1), and collect the 1:1 fraction The eluate is concentrated, cooled, filtered, and the filter cake is collected and dried to obtain the composition of the five alkaloids of the rhizome. The five alkaloids are dehydrosteiner's corydine, dehydroisoaporcarvetin, dehydroapporcavetin, coptisine and dehydrocarvetin. The total amount of the five alkaloids is 87%.
Embodiment 2
[0026] Take 2.0g of the crude extract of total alkaloids of Litectinus chinensis, add 80ml of 80% ethanol, heat and reflux for 1 hour, filter while it is hot, add 1.0g of silica gel to the filtrate, stir and evaporate to dryness, and then get the mixed sample silica gel; take 20g of silica gel with ethyl acetate After soaking in ester: ethanol (5:1), put it on the column by wet method, fill the sample silica gel on the top of the column, carry out gradient elution with ethyl acetate: ethanol (3:1-1:1), and collect the 1:1 fraction The eluate is concentrated, cooled, filtered, and the filter cake is collected and dried to obtain the composition of the five alkaloids of the rhizome. The five alkaloids are dehydrosteiner's corydine, dehydroisoaporcarvetin, dehydroapporcavetin, coptisine and dehydrocarvetin. The total amount of the five alkaloids is 92%.
Embodiment 3
[0028] Take 2.0g of the crude extract of total alkaloids of Litectinus chinensis, add 60ml of 90% ethanol, heat and reflux for 1 hour, filter while it is hot, add 4.0g of silica gel to the filtrate, stir and evaporate to dryness, and then get the mixed sample silica gel; take 16g of silica gel with ethyl acetate After soaking in ester: ethanol (1:1), put it on the column by wet method, fill the sample silica gel on the top of the column, carry out gradient elution with ethyl acetate: ethanol (3:1-1:1), and collect the 1:1 fraction The eluate is concentrated, cooled, filtered, and the filter cake is collected and dried to obtain the composition of the five alkaloids of the rhizome. The five alkaloids are dehydrosteiner's corydine, dehydroisoaporcarvetin, dehydroapporcavetin, coptisine and dehydrocarvetin. The total amount of the five alkaloids is 95%.
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