Paraffin sectioning method for Corydalis saxicola Bunting stems
A technology of paraffin sectioning and rock berberine, which is applied in the field of histology, can solve the problems of not clear enough root slices of rock berth, and the inability to identify the authenticity of medicinal materials, etc., to achieve clear structure, accurate embedding position, and obvious effect
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Embodiment 1
[0030] (1) Fixing: After harvesting the stems of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical stem is cut across, each piece is about 8mm long and wide 4mm, puts into the FAA fixative that the volume concentration is prepared by 70% alcohol and fixes for 24 hours, and the FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde The volume ratio is 90:5:5.
[0031] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 45 minutes; poured out a volume concentration of 80% alcohol, added a volume conc...
Embodiment 2
[0040] (1) Fixing: After harvesting the stems of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical root is cut across, each piece is about 5mm long and 3mm wide, and put into the FAA fixative prepared by 70% alcohol by volume concentration to fix for 24 hours. The FAA fixative heavy alcohol: glacial acetic acid: formaldehyde volume ratio is 90:5 :5.
[0041] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 55 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alcohol, vacuumed for 10...
Embodiment 3
[0050] (1) Fixing: After harvesting the stems of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical root is cut across, each piece is about 10mm long and 5mm wide, and put into the FAA fixative prepared by 70% alcohol by volume concentration to fix for 24 hours. The FAA fixative heavy alcohol: glacial acetic acid: formaldehyde volume ratio is 90:5 :5.
[0051] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 60 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alcohol, vacuumed for 10 ...
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