The invention discloses a paraffin sectioning method of roots of
corydalis saxicola bunting, which comprises sample fixing,
dehydration, transparentizing, paraffin impregnation, embedding, sectioning and expansion, deparaffinage,
dyeing and sealing. Before the transparentizing step, dehydrated roots of
corydalis saxicola bunting are placed into sarranine solution with
volume concentration of 0.8 to 1.2% to be dip-dyed for 8 to 12h; in the
dyeing step, a slide with the roots of
corydalis saxicola bunting is re-watered and then is placed into alcoholic solution to be subjected to color separation after re-watering, simultaneously,
acetic acid with a volume ratio of 1: (800 to 1,000) is added into the alcoholic solution for color separation, and finally, the obtained product is placed into fast green solution with
volume concentration of 0.8 to 1.2% to be dyed for 20 to 30 seconds. A root section prepared by the paraffin sectioning method disclosed by the invention is clearer in tissue, clear in profile root, and convenient in herbal
medicine identification.