High-efficiency induction method of Corydalis saxicola Bunting calluses

A technology for callus and Coptis chinensis, which is applied in the field of efficient callus induction of Coptidis chinensis, can solve problems such as the difficulty of large-scale propagation of wild Coptidis chinensis, and achieves the effects of solving difficulties in large-scale propagation, efficiently inducing, and accelerating the induction process.

Inactive Publication Date: 2017-05-31
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] An object of the present invention is to solve the problem that wild Litectin is difficu

Method used

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  • High-efficiency induction method of Corydalis saxicola Bunting calluses

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Embodiment 1

[0021] (1) Selection and disinfection of explants: take the leaves of Litectinus chinensis as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them in linear tap water for 5 minutes, disinfect the surface in 75% ethanol for 45 seconds, and then use 0.1% HgCl 2 Disinfect for 9 minutes, rinse with sterile water for 5 times, and finally absorb surface moisture with sterile filter paper to obtain leaf explants;

[0022] (2) Explants are induced to obtain callus: the leaf explants obtained in step (1) are inoculated into the induction medium for cultivation, and the induction medium is based on B5 medium as the basic medium, and the hormone 6-benzyl is also added Base adenine 1.1mg / L, 2,4-dichlorophenoxyacetic acid 1mg / L, VC20mg / L, activated carbon 0.2g / L, sucrose 30g / L and agar 4.5g / L, adjust the pH value of the induction medium 6.0, the culture temperature is 25°C, and the leaf explants are cultured in the dark for 1 week before being cultured in the li...

Embodiment 2

[0025] (1) Selection and disinfection of explants: Take the petiole of Litectinus chinensis as explants, soak in 2% aqueous solution of detergent for 10 minutes, rinse in linear tap water for 15 minutes, disinfect the surface in 75% ethanol for 30 seconds, and 0.1% HgCl 2 Disinfect for 8 minutes, rinse with sterile water for 3 times, and finally absorb the surface moisture with sterile filter paper to obtain petiole explants;

[0026] (2) Explant induction to obtain callus: inoculate the petiole explant obtained in step (1) into the induction medium and cultivate it. The induction medium is based on B5 medium as the basic medium, and the hormone 6-benzyl is also added. Base adenine 1.5mg / L, 2,4-dichlorophenoxyacetic acid 1.5mg / L, VC5mg / L, activated carbon 0.3g / L, sucrose 20g / L and agar 3.8g / L, adjust the pH value of the induction medium 6.0, the culture temperature is 23°C, the petiole explants are first cultured in the dark for 1 week and then light cultured, the light intens...

Embodiment 3

[0029] (1) Selection and disinfection of explants: take the stems of Litectinus chinensis as explants, soak in 2% aqueous solution of detergent for 7 minutes, rinse with linear tap water for 10 minutes, disinfect the surface in 75% ethanol for 40 seconds, and use 0.1% HgCl 2 Sterilize for 10 minutes, rinse with sterile water for 4 times, and finally absorb the surface moisture with sterile filter paper to obtain stem explants;

[0030] (2) Explant induction to obtain callus: the stem explant obtained in step (1) is inoculated into the induction medium for cultivation, and the induction medium is based on B5 medium as the basic medium, and hormone 6-benzyl is also added Base adenine 0.5mg / L, 2,4-dichlorophenoxyacetic acid 0.5mg / L, VC10mg / L, activated carbon 0.1g / L, sucrose 27g / L and agar 4.1g / L, adjust the pH value of the induction medium 6.0, the culture temperature is 26°C, the stem explants are first cultured in the dark for 1 week and then light cultured, the light intensit...

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Abstract

The invention discloses a high-efficiency induction method of Corydalis saxicola Bunting calluses. The method comprises the following steps: taking Corydalis saxicola Bunting explant, washing and disinfecting the Corydalis saxicola Bunting explant, inoculating an induction medium with the disinfected Corydalis saxicola Bunting explant, and carrying out induction culture for 10-15 d to obtain calluses, wherein the induction medium contains a B5 medium as a basal medium, and also contains 0.5-1.5 mg/L of 6-benzyladenine, 0.5-1.5 mg/L of 2,4-dichlorophenoxyacetic acid, 5-20 mg/L of vitamin C, 0.1-0.3 g/L of active carbon, 20-30 g/L of sucrose and 3.8-4.5 g/L of agar, and the pH value of the induction medium is 6.0. The method can be used to highly-efficiently induce the calluses and make the calluses rapidly propagate in order to solve the problem of difficulty in large-area breeding of wild Corydalis saxicola Bunting.

Description

technical field [0001] The invention relates to the field of plant tissue culture. More specifically, the present invention relates to a method for efficiently inducing callus of Litrichum chinensis. Background technique [0002] Euphorbia pekinensis Rupr., Euphorbia pekinensis Rupr., is a perennial evergreen herb with cylindrical roots and a length of 20-30 cm. The flowering period is May-August, and the fruiting period is June-September. Yanhuanglian is widely distributed in the whole country (except Taiwan, Yunnan, Tibet and Xinjiang), especially in the north, where it grows in hillsides, shrubs, roadsides, wastelands, grasses, forest margins and sparse forests. Litectin root can be used as medicine and has high medicinal value. It has the functions of dispelling water and laxative, reducing swelling and dissipating stagnation, treating edema mainly, and has the effect of dredging menstrual flow. It is used for the treatment of chronic nephritis, ascites of advanced schi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李翠张占江王一诺韦莹郭晓云韦坤华李林轩
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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