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Production method of high purity Corydalis saxicola Bunting alkaloid

A Coptidis Rhizoma alkaloid and production method technology, which is applied in the production field of high-purity Litectin Rhizoma Alkaloid, can solve the problems of not enough to ensure the safety of drug use and low alkaloid purity, and achieve the reduction of the amount of concentrated solvent, saving of extraction solvent, good effect

Active Publication Date: 2015-11-18
赵明东
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the purity of the alkaloids obtained by this method is not high enough to ensure the safety of medicines.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Dry and pulverize the rhizoma rhizome;

[0035] 2) Then feed into the enzymatic hydrolysis tank, add water 3 times the weight of the dry medicinal material, and then add a compound enzyme composed of cellulase and pectinase, the ratio of cellulase and pectinase in the compound enzyme is 1:0.5 , the dosage of the compound enzyme is 0.45%, and the enzymolysis is carried out at a temperature of 40° C. and a pH of 4.5 for 6 hours to obtain an enzymolysis mixture;

[0036] 3) Using ethanol with a volume concentration of 75% as the extraction solvent, extract the enzymatic hydrolysis mixture three times, the time is 2, 1.5, and 1 hour respectively, and the amount of each extraction solvent is 6, 5, and 4 times the weight of the enzymatic hydrolysis mixture respectively. Two extracts, recover ethanol until there is no ethanol smell, and concentrate to contain 2 g / l of dried medicinal materials in equivalent amount;

[0037] The third extraction is used as the first extract...

Embodiment 2

[0043] 1) Dry and pulverize the rhizoma rhizome;

[0044] 2) Then feed into the enzymatic hydrolysis tank, add water 3 times the weight of the dried medicinal material, and then add a compound enzyme composed of cellulase and pectinase, the ratio of cellulase and pectinase in the compound enzyme is 1:0.1 , the dosage of the compound enzyme is 0.1%, enzymolysis is carried out at a temperature of 40°C and a pH of 4.5 for 4 hours to obtain an enzymolysis mixture;

[0045] 3) Using ethanol with a volume concentration of 75% as the extraction solvent, extract the enzymatic hydrolysis mixture three times, the time is 2, 1.5, and 1 hour respectively, and the amount of each extraction solvent is 6, 5, and 4 times the weight of the enzymatic hydrolysis mixture respectively. Two extracts, recover ethanol until there is no ethanol smell, and concentrate to contain 2 g / l of dried medicinal materials in equivalent amount;

[0046] The third extraction is used as the first extraction solve...

Embodiment 3

[0052] 1) Dry and pulverize the rhizoma rhizome;

[0053] 2) Then feed into the enzymatic hydrolysis tank, add water 3 times the weight of the dry medicinal material, and then add a compound enzyme composed of cellulase and pectinase, the ratio of cellulase and pectinase in the compound enzyme is 1:3 , the dosage of the compound enzyme is 1%, and the enzymolysis is carried out at a temperature of 40° C. and a pH of 6 for 24 hours to obtain an enzymolysis mixture;

[0054] 3) Using ethanol with a volume concentration of 75% as the extraction solvent, extract the enzymatic hydrolysis mixture three times, the time is 2, 1.5, and 1 hour respectively, and the amount of each extraction solvent is 6, 5, and 4 times the weight of the enzymatic hydrolysis mixture respectively. Two extracts, recover ethanol until there is no ethanol smell, and concentrate to contain 2 g / l of dried medicinal materials in equivalent amount;

[0055] The third extraction is used as the first extraction so...

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Abstract

The invention provides a production method of high purity Corydalis saxicola Bunting alkaloid. The method mainly includes the steps of: 1) drying and crushing medicinal materials; 2) conducting enzymolysis on the compound enzyme composed of cellulose and pectinase; 3) using ethanol with a volume concentration of 50-90% as the extraction solvent to conduct extraction concentration; 4) carrying out centrifugal separation; 5) performing treatment with centrifugal separation to obtain crude total alkaloid; and 6) subjecting the crude total alkaloid to two-stage membrane filtration, thus obtaining a purified product. The method provided by the invention has the characteristics of simple and practicable operation, mild extraction process conditions, high extraction rate, small alkaloid loss in the separation and purification process, high recovery rate, and high product purity, and can obtain Corydalis saxicola Bunting alkaloid with a total alkaloid content of greater than 98%, thus completely meeting pharmacy requirement. Through detection of the product, the Corydalis saxicola Bunting alkaloid content is 98.6%, and the heavy metals, pesticide residues and microorganism are all accord with the national standard GB / T5009, 4789 standards.

Description

technical field [0001] The invention relates to the preparation of herbal medicines, in particular to lithotropia, and more specifically to a production method of high-purity lithotoptia alkaloids. Background technique [0002] Yanhuanglian (scientific name: Corydalissaxicola Bunting) is also known as: Yanhu, Yanlian, Chrysanthemum Coptidis, Shuanglian, and Lithian Huanglian. It is a perennial herbaceous plant of the family Papaveraceae. , mainly distributed in Gansu, Hubei, Guangxi, Sichuan, Guizhou, Yunnan and other places, while Guangxi is more common in Guangxi and Northwest Guangxi, with Donglan, Bama, Du'an, Jingxi and Debao more. Yanhuanglian is a characteristic Zhuang medicine in Guangxi [Lan Richun, Fan Liyong, Wei Zuogan. Classification and sources of 164 kinds of characteristic Zhuang medicines [J]. Chinese Journal of National Medicine, 2011, 17(12), 36-40], Yanhuanglian in Guizhou The two provinces of Guangxi, especially the northwestern region of Guangxi have a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/66
Inventor 赵明东林年秀
Owner 赵明东
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