46 results about "Chitosan binding" patented technology

A nanocomposite is disclosed comprising reduced graphene oxide (RGO) and at least one of a metal and an oxide of the metal. Also disclosed is an adsorbent comprising the nanocomposite and an adsorbent comprising the nanocomposite bound to silica by using chitosan. A filtering device comprising the nanocomposite and / or the adsorbent is also disclosed. Also disclosed are methods for producing the nanocomposites, adsorbents, and filtering devices described herein.

The invention discloses a waxberry coating preservative and a preparation method thereof and relates to the technical field of waxberry preservation. The preservative is prepared from the following raw materials: modified chitosan, plant essential oil, flaxseed gum, lysozyme, propolis, vitamin E, tea polyphenol, sucrose ester and water. According to the invention, the modified chitosan and various preservatives with anti-bacteria and insecticidal effects are combined for preparing the waxberry coating preservative; after the chitosan is chemically modified, the anti-bacteria property, permeability and water solubility are enhanced; the preservation effect is enhanced, the preservation time is prolonged and the preservative is nontoxic and residue-free; after the preservative disclosed by the invention is used for preserving waxberry, the refrigeration freshness period is 13-15 days and the shelf life under normal temperature is 6-7 days.

The invention relates to application of industrial sewage to microalgae aquaculture, in particular to a technical flow for pretreatment of eutrophic industrial sewage. A pretreatment technical flow for applying the industrial sewage to the microalgae aquaculture comprises the following steps: firstly, treating sludge in sewage by a technology of combining inorganic flocculant aluminium potassium sulfate and organic flocculant chitosan, wherein the mass ratio of the aluminium potassium sulfate to the chitosan is 100-200: 1, the molecular weight of the chitosan is 800-2000, and after the flocculants are added, the reaction time is controlled at 2 hours and the sedimentation time is controlled at 24 hours; secondly, adding sodium hydroxide into the treated sewage to regulate the pH value to 9, and filtering the sediment, wherein the sedimentation time is 12-24 hours; and thirdly, removing Cd, Cr, Hg, Pb and other heavy metal ions from water by using a molecular sieve column. The organic impurities are kept in the treated industrial sewage, and the inorganic impurities and the sludge are removed from the industrial sewage, so that the treated industrial sewage is applicable to the microalgae propagation.

The invention discloses a chitosan bonded organic-silica gel hybridization monolithic column and a preparation method thereof. The chitosan bonded organic-silica gel hybridization monolithic column is prepared by combining the following components in percentage by mass with chitosan: 24% of a functional monomer with an amide group, 6% of a cage-like polysilsesquioxane cross-linking agent, 1.0% of an initiator and 69.0% of a pore-foaming agent. The invention further provides a preparation method of the chitosan bonded organic-silica gel hybridization monolithic column. The chitosan bonded organic-silica gel hybridization monolithic column disclosed by the invention has a nano cage-like siloxane structure, and is high in specific surface function and biocompatibility, and rich in hydroxyl and amino due to post-column bonded chitosan so as to provide abundant hydrogen bonds and perform hydrophilic interaction, thereby being applicable to analysis and separation of carbohydrates such as monosaccharide and disaccharide with high hydrophilcity.

Provided are an antiacid nano particle oral deoxyribonucleic acid (DNA) anti-tumor vaccine with the potential of hydrogen (pH) sensitive characteristic and a preparation method. The DNA oral vaccine is nano particles formed by combining chitosan with alginic acid for surface finish and encoded tumor specific antigen Legumain protein DNA plasmids. The DNA oral vaccine is capable of being efficiently phagocytized by dendritic cells and macrophage in peyer's patches and expressing the encoded tumor antigen to activate immune damage of a host for tumor cells. The nano particles have small toxic and side effects and a strong antigen presentation role. Specifically, the DNA plasmids and chitosan are led to be in static negative crosslinking, and then, alginic acid is used for surface finish of chitosan nano particles. By means of immunofluorescent staining and flow cytometry, growth and transfer conditions of breast cancer of a mouse are estimated, immune response of the mouse is analyzed, and antigen presentation capability and anti-tumor effect of the antiacid nano particle oral DNA anti-tumor vaccine with the potential of pH sensitive characteristic are determined.

The invention discloses a treating reagent applicable to high-salt pesticide wastewater. The treating reagent comprises the following raw materials by weight: 50 to 90 parts of rice straw, 50 to 60 parts of raw attapulgite ore, 80 to 100 parts of dilute hydrochloric acid, 70 to 90 parts of aluminum sulfate and 8 to 15 parts of chitosan. The treating reagent provided by the invention combines biomass charcoal, modified attapulgite and chitosan for treatment of the wastewater, so recycling of agricultural waste is realized, and a novel environment-friendly effective flocculating agent is provided for treatment of industrial wastewater; the flocculating agent prepared in the invention has excellent coacervation capability, enables the concentration of high-salt substances and high-toxicity organic matters in the pesticide wastewater to be obviously decreased and effectively overcomes the disadvantages of great usage amounts of inorganic flocculating agents and high toxicity of organic flocculating agents in conventional wastewater treatment processes; and the treating reagent applicable to high-salt pesticide wastewater is worth promotion.

The invention discloses an air-permeable moisturizing dressing with bacteriostasis and drug percutaneous absorption promotion functions and a preparation method of the air-permeable moisturizing dressing. The air-permeable moisturizing dressing comprises the following raw materials in parts by weight: 12-14 parts of N-trimethyl chitosan, 8-12 parts of menthanol, 5-7 parts of isopropyl myristate, 20-30 parts of chitosan, 25-35 parts of loofah sponge fibers, 8-14 parts of isomalto-oligosaccharide, 5-9 parts of polyvinyl alcohol, 6-10 parts of carboxymethyl cellulose sodium, 10-12 parts of glycerol, 8-10 parts of mannitol, 12-20 parts of medicinal powder, 3-7 parts of weak-acid calcium chloride and a proper amount of deionized water. As chitosan and loofah sponge fibers are combined together, the moisturizing property of the dressing is guaranteed by adsorbability of chitosan, and the air permeability of the dressing is guaranteed by the hollow tube bundle structures of the loofah sponge fibers; meanwhile, chitosan derivatives are used together to effectively promote percutaneous absorption and wound healing, so that a favorable effect is achieved.

The invention discloses a composite photocatalysis material for degrading rhodamine B. With magnetic Fe3O4 as a substrate, a magnetism technology is combined with high-adsorption and high-activity chitosan (CS), the surface of the substrate is wrapped by the chitosan to obtain magnetic chitosan microspheres, the surface of the chitosan contains rich amidogen and hydroxyl and has high coordinationcapacity, the amidogen and hydroxyl are used for loading Al3+ ions, through NaBH4, the microspheres are subjected to reduction into metal nano particles Al, and the composite photocatalysis material is obtained. The prepared composite photocatalysis material is high in activity, good in dispersion stability and low in cost and is easily separated and recycled, the experiment process is simple, andthe material has important application prospects in the field of catalytic degradation of wastewater with the rhodamine B.

A drug carrier is provided with a structure of a lipid shell enclosing aqueous micelles. The lipid shell includes lipid and emulsifier, in which the emulsifier encloses the lipid. The components of the aqueous micelles are phospholipids and amphiphilic chitosan, and the aqueous micelles enclose an aqueous solution containing a drug. Furthermore, the method of preparing the drug carrier is also provided. Therefore, with the pharmaceutical advantages of lipid-based nanoparticle included low drug leakage and the ability of to overcome the multiple drug resistance, this new formulation were further incorporated with the chitosan and featured with high payload efficiency. The features could enhance intracellular concentration of anti-cancer drug and oral bioavailability.

The invention discloses a chitosan bonded organic-silica gel hybridization monolithic column and a preparation method thereof. The chitosan bonded organic-silica gel hybridization monolithic column is prepared by combining the following components in percentage by mass with chitosan: 24% of a functional monomer with an amide group, 6% of a cage-like polysilsesquioxane cross-linking agent, 1.0% of an initiator and 69.0% of a pore-foaming agent. The invention further provides a preparation method of the chitosan bonded organic-silica gel hybridization monolithic column. The chitosan bonded organic-silica gel hybridization monolithic column disclosed by the invention has a nano cage-like siloxane structure, and is high in specific surface function and biocompatibility, and rich in hydroxyl and amino due to post-column bonded chitosan so as to provide abundant hydrogen bonds and perform hydrophilic interaction, thereby being applicable to analysis and separation of carbohydrates such as monosaccharide and disaccharide with high hydrophilcity.

The invention relates to a method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering, and belongs to the field of large molecule detection. The method is capableof avoiding technical defects in the prior art that quantitative detection of the content of chitosan of finished products is difficult to realize, and operation technology is complex. According to the method, at certain conditions, brilliant blue dye is combined with chitosan so as to form an ionic associate capable of enhancing resonance Rayleigh scattering signals in a system, and two characteristic peaks at 345nm and 445nm are observed. Under preset conditions, when the concentration of chitosan ranges from 0.005 to 1.8<mu>g / ml, the linear relationship of the concentration of chitosan with resonance Rayleigh scattering strength is excellent, so that the dual-wavelength resonance Rayleigh scattering method used for measuring chitosan content is established. The dual-wavelength resonance Rayleigh scattering is influenced by the molecular weight of chitosan; the adopted reagents are cheap; the sensitivity, the reproducibility, and the anti-interference capacity are high, and operation is convenient.

The invention discloses a folic acid-chitosan-nanometer selenium tumor targeted drug delivery system and a preparation method thereof. The targeted drug delivery system is composed of folic acid, chitosan and nanometer selenium. Through activation the folic acid, the folic acid and the chitosan are combined to serve as a stabilizer and a coating agent, and then combined with the nanometer seleniumto form the folic acid-chitosan-nanometer selenium tumor targeted drug delivery system with a tumor targeting effect. The targeted drug delivery system prepared by using the preparation method provided by the invention is small in average particle size, uniform in particle size distribution and stable in properties; and the method is easy to be implemented, easy to operate and high in repeatability.

The invention discloses a composite nano-adsorption material, a preparation method and an application thereof and belongs to the field of nanomaterials. The preparation method includes: preparing Fe3O4 nanoparticles, preparing Fe3O4@SiO nanoparticles, preparing Fe3O4 composite nano-adsorptive material. In the invention, by combining the nano-magnetic ions with chitosan, the adsorption efficiency of the composite nano-adsorptive material is improved by means of the large specific surface area of the chitosan; in addition, after the material adsorbs the heavy metal ion, the material can be rapidly recycled under a low-intensity magnetic field, then the material is washed to remove the heavy metal ions for recovery. Besides, by modification and crosslinking of the chitosan, the hydrophobilityof the chitosan is improved, and hydrolysis degree of the chitosan in an acidic condition is reduced, and performance stability during recycling is enhanced.

The invention relates to the technical field of hydrogel composite materials, and discloses a hydrogel composite material with a porous structure as well as preparation and application thereof. The hydrogel composite material is CNF / CC / Eu / TTA, and a rare earth complex formed by TTA and Eu is connected with a three-dimensional network structure formed by CNF and CC in a covalent bond mode. The CNFis cellulose nanofibers, the CC is carboxylated chitosan, the Eu is rare earth europium ions, and the TTA is sodium salt obtained after deprotonation of 2-thiophene formyl trifluoroacetone. The cellulose nanofibers are combined with the carboxylated chitosan through hydrogen bonds. The rare earth europium ions are coordinated with carboxyl functional groups of the carboxylated chitosan, so that the rare earth europium ions are stably connected with a hydrogel network skeleton in a covalent bond mode, and the TTA is further coordinated with the rare earth europium ions to form an excellent redfluorescent hydrogel material which shows excellent compressive strain performance and can be used as an environment-friendly recognition material.

The invention relates to a method for preparing a ferritin-chitosan complex by utilizing transglutaminase and chitosan. The method comprises the following steps: taking ferritin extracted from red beans as a raw material, binding chitosan onto the ferritin by utilizing a catalytic action of the transglutaminase, and separating effective binding components by a gel filtration method, thereby obtaining the ferritin-chitosan complex. The prepared ferritin-chitosan complex has high stability compared with the ferritin. According to the method, the chitosan is catalyzed to be covalently bound ontothe ferritin shell by utilizing the transglutaminase, the heat stability and gastrointestinal digestion stability of the ferritin are improved, and the bioavailability and iron supplement efficiency of the ferritin are further improved. The ferritin-chitosan complex prepared by the method can be applied to the industries such as food additives, feed, health foods and the like.

The invention discloses a preparation method of a wastewater degrading purifying agent for livestock and poultry farming. The purifying agent is prepared from chitosan, a soluble high polymer material, cationic starch, dimethyl diallyl ammonium chloride, pulverized fuel ash, chromic nitrate, cerous nitrate, aluminum oxide powder and other raw materials. A coating-type double-layer structure is adopted, on an outer layer, chitosan is combined with the water soluble high polymer material, adsorbing and degrading particles of an internal core need to be slowly released into a water body through organic layer pores in the outer layer, in this way, influences on the water body are small, and acting time is delayed; the pulverized fuel ash is used for being treated separately through acid and base and then serves as a substrate supported Cr-Ce compound oxidizing body, in this way, a degrading space is provided for organic pollution, and substances of types capable of being adsorbed and degraded by organic matter are substantially increased. The purifying agent as an effective degrading material is provided for kinds of large-quantity pollutants in the wastewater of livestock and poultryfarming, the method has the advantages of being easy to operate, high in degrading speed, long in maintenance period and others, remarkable social, economic and environmental benefits can be generated, and the purifying agent is widely applied to kinds of livestock and poultry farming enterprises.

The invention belongs to the field of environment-friendly materials, and particularly relates to a polyethylene foam material and a preparation method thereof. The polyethylene foam material comprises the following raw materials: LDPE, a foaming agent, a cross-linking agent and chitosan modified by sodium lignin sulfonate, wherein the chitosan modified by the sodium lignin sulfonate is obtained by modifying chitosan by the sodium lignin sulfonate under an acidic condition. According to the polyethylene foam material provided by the invention, the chitosan modified by sodium lignin sulfonate is combined with the cross-linking agent, the foaming agent and a small amount of LDPE, all the raw materials play a synergistic effect, and on the premise that the dosage of LDPE is reduced, the prepared foam material is stable in chemical performance, excellent in mechanical performance, recyclable and easy to degrade. In addition, the chitosan and the sodium lignin sulfonate are wide in source, easy to obtain and low in cost.

The invention discloses a method using the synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water. The method comprises the following steps: (1) preparing a lead-cadmium-mercury mixed adsorption solution, a lead ion adsorption solution, a cadmium ion adsorption solution, and a mercury ion adsorption solution; (2) respectively taking chitosan, bacillus subtilis, and a mixture of chitosan and bacillus subtilis as the heavy metal absorbent to adsorb the adsorption solutions in the step (1), and comparing the adsorbing effect of three adsorbents. The invention researches the synergetic adsorption effect of chitosan and bacillus subtilis on heavy metals (lead, cadmium, and mercury). When chitosan and bacillus subtilis are used together, chitosan can form a thick and loosened layer on the surface of bacillus subtilis, metal ions can freely enter the layer and carry out chelation reactions; due to the large surface area of bacteria, the chitosan can fully spread, more binding sites are exposed, and thus the heavy metal adsorption performance is largely improved.

Provided is a novel antimicrobial composition, which is against an infectious microorganism causing a respiratory infection, using natural food additives with high safety. The antimicrobial composition comprises a complex, in which lysozyme is bound to chitosan, and has an antimicrobial effect against an infectious microorganism inducing a respiratory infection.

The invention relates to a method for retaining freshness of summer black seedless grape fruit by combination of a pogostemon cablin extract and chitosan, and belongs to the technical field of fruit freshness retaining. The method is characterized by comprising the following steps: soaking 90-percent mature summer black seedless grape fruits which have consistent sizes and colors, are free of pest and disease damage and mechanical injury and have fruit stems with the pogostemon cablin extract, and performing coating treatment with chitosan treatment liquid. According to the summer black seedless grape fruit freshness retaining method, the storage quality of the summer black seedless grape fruits can be improved, and the storage period can be prolonged; after the summer black seedless grape fruits are treated, the freshness retaining period at normal temperature can reach 25 to 30 days, which is prolonged by 18 to 23 days when as compared with the freshness retaining period of non-treated fruits, and the rotting rate can be lowered to 20 percent or below; furthermore, high sensory quality of the summer black seedless grape fruits is retained; and a material for treatment is nontoxic and harmless.

The invention provides an invisible orthodontic appliance and a preparation method thereof. The invisible orthodontic appliance is obtained by introducing a coating material into the surface of an invisible orthodontic base material through an ultrasonic-assisted technology. The coating material comprises villiaumite, PEG (polyethylene glycol), chitosan and nano titanium dioxide, and the coating material is prepared by loading fluorine on the PEG, so that a stable and lasting fluorine slow-release effect is obtained, and the coating material has the performance of promoting enamel mineralization; the PEG can also improve the dispersity of nano titanium dioxide particles in the suspension and improve the dispersion uniformity of nano titanium dioxide; the nano titanium dioxide has good biocompatibility, stable chemical property and remarkable antibacterial effect, and the antibacterial property can be remarkably enhanced by combining with chitosan, the obtained invisible orthodontic appliance has the characteristics of excellent mechanical property, long-acting bacteriostasis and promotion of enamel mineralization.

Provided are an antiacid nano particle oral deoxyribonucleic acid (DNA) anti-tumor vaccine with the potential of hydrogen (pH) sensitive characteristic and a preparation method. The DNA oral vaccine is nano particles formed by combining chitosan with alginic acid for surface finish and encoded tumor specific antigen Legumain protein DNA plasmids. The DNA oral vaccine is capable of being efficiently phagocytized by dendritic cells and macrophage in peyer's patches and expressing the encoded tumor antigen to activate immune damage of a host for tumor cells. The nano particles have small toxic and side effects and a strong antigen presentation role. Specifically, the DNA plasmids and chitosan are led to be in static negative crosslinking, and then, alginic acid is used for surface finish of chitosan nano particles. By means of immunofluorescent staining and flow cytometry, growth and transfer conditions of breast cancer of a mouse are estimated, immune response of the mouse is analyzed, and antigen presentation capability and anti-tumor effect of the antiacid nano particle oral DNA anti-tumor vaccine with the potential of pH sensitive characteristic are determined.

The invention discloses a lipase immobilization method. The lipase immobilization method comprises the steps that lipase, a protein linking peptide and a chitosan binding domain amino acid sequence are sequentially connected to form a lipase fused protein, chitosan is used as a carrier to fix the lipase fused protein, and fixed lipase is obtained. The fixed lipase is subjected to recycling of fifth times, the conversion rate of cetyl palmitate catalyzed by the fixed lipase fused protein can be still kept 90%. The method can achieve large scale production, the lipase production can be improved, and the production cost can be reduced.

The invention relates to a method for preparing a ferritin-chitosan complex by using transglutaminase and chitosan, the steps are as follows: the method uses ferritin extracted from red beans as a raw material, and utilizes the catalysis of transglutaminase , chitosan is combined on ferritin, and the effective binding component is separated by gel filtration method to obtain ferritin-chitosan complex, and the obtained ferritin-chitosan complex has Greater stability. The method utilizes transglutaminase to catalyze the covalent binding of chitosan on the shell of ferritin to improve the thermal stability and gastrointestinal digestion stability of ferritin, thereby improving the bioavailability and iron supplementation efficiency of ferritin. The obtained ferritin-chitosan complex can be applied in industries such as food additives, feed, health food and the like.

The invention discloses a preparation method of a magnetic chitosan modified straw composite adsorbent and an obtained product and application. The composite adsorbent is prepared by compounding and crosslinking an activated straw powder, chitosan and CoFe2O4. The large-skeleton straw and the high-adsorption capacity chitosan are combined for the first time, the effective utilization value is found for the waste of biomass, the adsorption property of the adsorbent is improved, and the cost of the adsorbent is also lowered. The preparation method is simple, does not need the high-energy consumption processes like high-temperature pyrolysis, and does not need other complicated processes or expensive chemical agents, the composite adsorbent is loose and fluffy, the adsorbing capacity is high,the adsorption process is simple and feasible, the adsorbent is convenient to separate, no secondary pollution is caused after the adsorption, the adsorbent can be repeatedly used, and the compositeadsorbent is an economical green environmentally friendly novel adsorbent material and has a broad application prospect.

Provided is an antibacterial composition against Acinetobacter. Provided are: a pharmaceutical composition which is for treating or preventing Acinetobacter infection, contains a complex in which lysozyme and chitosan are bound, and has an antibacterial property against Acinetobacter; and a pharmaceutical composition which is for treating or preventing Acinetobacter infection and contains a complex in which lysozyme and chitosan are bound.

The composite microsphere preparation of lactic acid-hydroxyacetic acid copolymer consists of medicine, calcium alginate, chitosan, glycolide-lactide copolymer, emulsifier and medicine supplementary material. It is prepared through adding low concentration sodium alginate solution to inner water phase containing medicine, forming initial emulsion with PLGA-containing organic phase, injecting PVA solution with chitosan and calcium chloride to form complex emulsion, decompression rotary evaporation to cure, water washing and freeze drying. The process is simple and feasible, and has no influenceon the size of microsphere and no change in protein encapsulating rate, capacity of forming one hydrophilic micro environment to protect protein medicine, and capacity of reducing the release of medicine.

The invention discloses a nanosystem-based water treatment agent and a preparation method thereof, and belongs to the technical field of reclaimed water treatment. The preparation method comprises thefollowing steps: winding, stretching and rearranging a prepared polyoxometallate (POM) nanocrystal sheet to convert the POM nanocrystal sheet into single-wall POM nanotubes, combining the nanotubes with modified chitosan to form a composite material, and finally assembling the prepared nano zero-valent iron into the POM nanotubes to prepare the composite agent. The prepared agent utilizes the modified nano zero-valent iron to adsorb and reduce nitrate, so the limitation of conventional zero-valent iron nitrate removal on pH and other conditions is broken through, and the phenomena of passivation, agglomeration and the like generated in the removal process are also overcome, thereby the high removal efficiency is achieved.

The invention provides an invisible appliance and a preparation method thereof. The invisible appliance is obtained by introducing a coating material into the surface of an invisible orthodontic substrate through an ultrasonic-assisted technology; the coating material includes fluorine salt, PEG, chitosan Sugar and nano-titanium dioxide, the preparation of coating material adopts PEG-loaded fluorine to obtain stable and long-lasting slow-release fluorine effect, so that it has the property of promoting enamel mineralization; PEG can also improve the dispersion of nano-titanium dioxide particles in suspension, improve the Nano-titanium dioxide has uniform dispersion, good biocompatibility, stable chemical properties, and remarkable bacteriostatic effect. Its combination with chitosan can significantly enhance bacteriostatic properties. The obtained invisible appliance has excellent mechanical properties, long-term bacteriostatic, Features that promote enamel mineralization.

The invention relates to the technical field of hydrogel composite materials, and discloses a porous structure hydrogel composite material and its preparation and application. The hydrogel composite material is CNF / CC / Eu / TTA, and the rare earth complex formed by TTA and Eu is connected with the three-dimensional network structure formed by CNF and CC by covalent bond. Wherein, the CNF is a cellulose nanofiber, CC is a carboxylated chitosan, Eu is a rare earth europium ion, and TTA is a deprotonated sodium salt of 2-thiopheneformyl trifluoroacetone. Cellulose nanofibers bind to carboxylated chitosan through hydrogen bonding. The rare earth europium ion is coordinated with the carboxyl functional group of the carboxylated chitosan, so that it is stably connected to the hydrogel network skeleton in a covalent bond manner. TTA is then further coordinated with the rare earth europium ion to form an excellent red fluorescent hydrogel material. It exhibits excellent compressive strain properties and can be used as an environmentally friendly identification material.