46 results about "Chitosan binding" patented technology

The invention discloses a chitosan bonded organic-silica gel hybridization monolithic column and a preparation method thereof. The chitosan bonded organic-silica gel hybridization monolithic column is prepared by combining the following components in percentage by mass with chitosan: 24% of a functional monomer with an amide group, 6% of a cage-like polysilsesquioxane cross-linking agent, 1.0% of an initiator and 69.0% of a pore-foaming agent. The invention further provides a preparation method of the chitosan bonded organic-silica gel hybridization monolithic column. The chitosan bonded organic-silica gel hybridization monolithic column disclosed by the invention has a nano cage-like siloxane structure, and is high in specific surface function and biocompatibility, and rich in hydroxyl and amino due to post-column bonded chitosan so as to provide abundant hydrogen bonds and perform hydrophilic interaction, thereby being applicable to analysis and separation of carbohydrates such as monosaccharide and disaccharide with high hydrophilcity.

Provided are an antiacid nano particle oral deoxyribonucleic acid (DNA) anti-tumor vaccine with the potential of hydrogen (pH) sensitive characteristic and a preparation method. The DNA oral vaccine is nano particles formed by combining chitosan with alginic acid for surface finish and encoded tumor specific antigen Legumain protein DNA plasmids. The DNA oral vaccine is capable of being efficiently phagocytized by dendritic cells and macrophage in peyer's patches and expressing the encoded tumor antigen to activate immune damage of a host for tumor cells. The nano particles have small toxic and side effects and a strong antigen presentation role. Specifically, the DNA plasmids and chitosan are led to be in static negative crosslinking, and then, alginic acid is used for surface finish of chitosan nano particles. By means of immunofluorescent staining and flow cytometry, growth and transfer conditions of breast cancer of a mouse are estimated, immune response of the mouse is analyzed, and antigen presentation capability and anti-tumor effect of the antiacid nano particle oral DNA anti-tumor vaccine with the potential of pH sensitive characteristic are determined.

The invention discloses an air-permeable moisturizing dressing with bacteriostasis and drug percutaneous absorption promotion functions and a preparation method of the air-permeable moisturizing dressing. The air-permeable moisturizing dressing comprises the following raw materials in parts by weight: 12-14 parts of N-trimethyl chitosan, 8-12 parts of menthanol, 5-7 parts of isopropyl myristate, 20-30 parts of chitosan, 25-35 parts of loofah sponge fibers, 8-14 parts of isomalto-oligosaccharide, 5-9 parts of polyvinyl alcohol, 6-10 parts of carboxymethyl cellulose sodium, 10-12 parts of glycerol, 8-10 parts of mannitol, 12-20 parts of medicinal powder, 3-7 parts of weak-acid calcium chloride and a proper amount of deionized water. As chitosan and loofah sponge fibers are combined together, the moisturizing property of the dressing is guaranteed by adsorbability of chitosan, and the air permeability of the dressing is guaranteed by the hollow tube bundle structures of the loofah sponge fibers; meanwhile, chitosan derivatives are used together to effectively promote percutaneous absorption and wound healing, so that a favorable effect is achieved.

The invention discloses a composite photocatalysis material for degrading rhodamine B. With magnetic Fe3O4 as a substrate, a magnetism technology is combined with high-adsorption and high-activity chitosan (CS), the surface of the substrate is wrapped by the chitosan to obtain magnetic chitosan microspheres, the surface of the chitosan contains rich amidogen and hydroxyl and has high coordinationcapacity, the amidogen and hydroxyl are used for loading Al3+ ions, through NaBH4, the microspheres are subjected to reduction into metal nano particles Al, and the composite photocatalysis material is obtained. The prepared composite photocatalysis material is high in activity, good in dispersion stability and low in cost and is easily separated and recycled, the experiment process is simple, andthe material has important application prospects in the field of catalytic degradation of wastewater with the rhodamine B.

The invention relates to a method used for measuring chitosan content through dual-wavelength resonance Rayleigh scattering, and belongs to the field of large molecule detection. The method is capableof avoiding technical defects in the prior art that quantitative detection of the content of chitosan of finished products is difficult to realize, and operation technology is complex. According to the method, at certain conditions, brilliant blue dye is combined with chitosan so as to form an ionic associate capable of enhancing resonance Rayleigh scattering signals in a system, and two characteristic peaks at 345nm and 445nm are observed. Under preset conditions, when the concentration of chitosan ranges from 0.005 to 1.8<mu>g/ml, the linear relationship of the concentration of chitosan with resonance Rayleigh scattering strength is excellent, so that the dual-wavelength resonance Rayleigh scattering method used for measuring chitosan content is established. The dual-wavelength resonance Rayleigh scattering is influenced by the molecular weight of chitosan; the adopted reagents are cheap; the sensitivity, the reproducibility, and the anti-interference capacity are high, and operation is convenient.

The invention discloses a folic acid-chitosan-nanometer selenium tumor targeted drug delivery system and a preparation method thereof. The targeted drug delivery system is composed of folic acid, chitosan and nanometer selenium. Through activation the folic acid, the folic acid and the chitosan are combined to serve as a stabilizer and a coating agent, and then combined with the nanometer seleniumto form the folic acid-chitosan-nanometer selenium tumor targeted drug delivery system with a tumor targeting effect. The targeted drug delivery system prepared by using the preparation method provided by the invention is small in average particle size, uniform in particle size distribution and stable in properties; and the method is easy to be implemented, easy to operate and high in repeatability.

The invention discloses a composite nano-adsorption material, a preparation method and an application thereof and belongs to the field of nanomaterials. The preparation method includes: preparing Fe3O4 nanoparticles, preparing Fe3O4@SiO nanoparticles, preparing Fe3O4 composite nano-adsorptive material. In the invention, by combining the nano-magnetic ions with chitosan, the adsorption efficiency of the composite nano-adsorptive material is improved by means of the large specific surface area of the chitosan; in addition, after the material adsorbs the heavy metal ion, the material can be rapidly recycled under a low-intensity magnetic field, then the material is washed to remove the heavy metal ions for recovery. Besides, by modification and crosslinking of the chitosan, the hydrophobilityof the chitosan is improved, and hydrolysis degree of the chitosan in an acidic condition is reduced, and performance stability during recycling is enhanced.

The invention relates to the technical field of hydrogel composite materials, and discloses a hydrogel composite material with a porous structure as well as preparation and application thereof. The hydrogel composite material is CNF/CC/Eu/TTA, and a rare earth complex formed by TTA and Eu is connected with a three-dimensional network structure formed by CNF and CC in a covalent bond mode. The CNFis cellulose nanofibers, the CC is carboxylated chitosan, the Eu is rare earth europium ions, and the TTA is sodium salt obtained after deprotonation of 2-thiophene formyl trifluoroacetone. The cellulose nanofibers are combined with the carboxylated chitosan through hydrogen bonds. The rare earth europium ions are coordinated with carboxyl functional groups of the carboxylated chitosan, so that the rare earth europium ions are stably connected with a hydrogel network skeleton in a covalent bond mode, and the TTA is further coordinated with the rare earth europium ions to form an excellent redfluorescent hydrogel material which shows excellent compressive strain performance and can be used as an environment-friendly recognition material.

The invention discloses a preparation method of a wastewater degrading purifying agent for livestock and poultry farming. The purifying agent is prepared from chitosan, a soluble high polymer material, cationic starch, dimethyl diallyl ammonium chloride, pulverized fuel ash, chromic nitrate, cerous nitrate, aluminum oxide powder and other raw materials. A coating-type double-layer structure is adopted, on an outer layer, chitosan is combined with the water soluble high polymer material, adsorbing and degrading particles of an internal core need to be slowly released into a water body through organic layer pores in the outer layer, in this way, influences on the water body are small, and acting time is delayed; the pulverized fuel ash is used for being treated separately through acid and base and then serves as a substrate supported Cr-Ce compound oxidizing body, in this way, a degrading space is provided for organic pollution, and substances of types capable of being adsorbed and degraded by organic matter are substantially increased. The purifying agent as an effective degrading material is provided for kinds of large-quantity pollutants in the wastewater of livestock and poultryfarming, the method has the advantages of being easy to operate, high in degrading speed, long in maintenance period and others, remarkable social, economic and environmental benefits can be generated, and the purifying agent is widely applied to kinds of livestock and poultry farming enterprises.

The invention belongs to the field of environment-friendly materials, and particularly relates to a polyethylene foam material and a preparation method thereof. The polyethylene foam material comprises the following raw materials: LDPE, a foaming agent, a cross-linking agent and chitosan modified by sodium lignin sulfonate, wherein the chitosan modified by the sodium lignin sulfonate is obtained by modifying chitosan by the sodium lignin sulfonate under an acidic condition. According to the polyethylene foam material provided by the invention, the chitosan modified by sodium lignin sulfonate is combined with the cross-linking agent, the foaming agent and a small amount of LDPE, all the raw materials play a synergistic effect, and on the premise that the dosage of LDPE is reduced, the prepared foam material is stable in chemical performance, excellent in mechanical performance, recyclable and easy to degrade. In addition, the chitosan and the sodium lignin sulfonate are wide in source, easy to obtain and low in cost.

The invention discloses a method using the synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water. The method comprises the following steps: (1) preparing a lead-cadmium-mercury mixed adsorption solution, a lead ion adsorption solution, a cadmium ion adsorption solution, and a mercury ion adsorption solution; (2) respectively taking chitosan, bacillus subtilis, and a mixture of chitosan and bacillus subtilis as the heavy metal absorbent to adsorb the adsorption solutions in the step (1), and comparing the adsorbing effect of three adsorbents. The invention researches the synergetic adsorption effect of chitosan and bacillus subtilis on heavy metals (lead, cadmium, and mercury). When chitosan and bacillus subtilis are used together, chitosan can form a thick and loosened layer on the surface of bacillus subtilis, metal ions can freely enter the layer and carry out chelation reactions; due to the large surface area of bacteria, the chitosan can fully spread, more binding sites are exposed, and thus the heavy metal adsorption performance is largely improved.

The invention provides an invisible orthodontic appliance and a preparation method thereof. The invisible orthodontic appliance is obtained by introducing a coating material into the surface of an invisible orthodontic base material through an ultrasonic-assisted technology. The coating material comprises villiaumite, PEG (polyethylene glycol), chitosan and nano titanium dioxide, and the coating material is prepared by loading fluorine on the PEG, so that a stable and lasting fluorine slow-release effect is obtained, and the coating material has the performance of promoting enamel mineralization; the PEG can also improve the dispersity of nano titanium dioxide particles in the suspension and improve the dispersion uniformity of nano titanium dioxide; the nano titanium dioxide has good biocompatibility, stable chemical property and remarkable antibacterial effect, and the antibacterial property can be remarkably enhanced by combining with chitosan, the obtained invisible orthodontic appliance has the characteristics of excellent mechanical property, long-acting bacteriostasis and promotion of enamel mineralization.

The invention discloses a lipase immobilization method. The lipase immobilization method comprises the steps that lipase, a protein linking peptide and a chitosan binding domain amino acid sequence are sequentially connected to form a lipase fused protein, chitosan is used as a carrier to fix the lipase fused protein, and fixed lipase is obtained. The fixed lipase is subjected to recycling of fifth times, the conversion rate of cetyl palmitate catalyzed by the fixed lipase fused protein can be still kept 90%. The method can achieve large scale production, the lipase production can be improved, and the production cost can be reduced.