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Method using synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water

A Bacillus subtilis and synergistic technology, applied in chemical instruments and methods, water pollutants, water/sewage treatment, etc., can solve the problems of increased investment costs, single types of heavy metal ions, and large capital investment, and achieve economical prices, Wide range of material sources and good adsorption effect

Pending Publication Date: 2016-09-14
NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The adsorption method also includes chemical adsorption, biological adsorption and physical adsorption. The single chemical adsorption, physical adsorption or microbial adsorption is generally poor in the treatment of low-concentration heavy metals, and cannot meet the wastewater discharge standards. Adsorbents with better treatment effects are expensive , a lot of capital investment; the heavy metal ions that can be adsorbed by the adsorbent are single, which indirectly increases the investment cost
At present, there are few reports on the comprehensive use of two or three adsorption methods in chemical adsorption, physical adsorption and microbial adsorption in the prior art.

Method used

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  • Method using synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water
  • Method using synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water
  • Method using synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water

Examples

Experimental program
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Effect test

Embodiment 3

[0059] Using chitosan and Bacillus subtilis as adsorbents, investigate the synergistic effect of chitosan and Bacillus subtilis on the adsorption effect of lead, cadmium and mercury heavy metals. Measure 100mL of lead, cadmium and mercury mixed adsorption with a concentration of 20ug / mL in a graduated cylinder. solution in three Erlenmeyer flasks, adjust the pH of the adsorption solution in each Erlenmeyer flask with hydrochloric acid solution and sodium hydroxide solution, and adjust their pH to 7.0, and then use inductively coupled plasma-atom The emission (ICP-AES) spectrometer separately measures the concentration C 1 , add 6,000,000 strains of Bacillus subtilis and 0.2g chitosan respectively in each Erlenmeyer flask, seal the bottle mouth of each Erlenmeyer flask respectively with plastic wrap, then place the Erlenmeyer flask in a constant temperature shaker to vibrate, vibrate The speed is 120r / min, the oscillation temperature is 25°C; the oscillation is stopped after th...

Embodiment 4

[0066] Measure 100mL of lead ion adsorption solution, cadmium ion adsorption solution and mercury ion adsorption solution with a concentration of 20ug / mL in three conical flasks, and adjust the adsorption in each conical flask with hydrochloric acid solution and sodium hydroxide solution respectively. The pH of the solution, their pH is adjusted to 7.0, add 0.2g chitosan respectively, seal the bottleneck of each Erlenmeyer flask respectively with plastic wrap, then the Erlenmeyer flask is placed in a constant temperature oscillator to vibrate, the vibration speed The shaking temperature was 120r / min, and the shaking temperature was 25°C; after shaking for 8 hours, the shaking was stopped, and the mixture in each Erlenmeyer flask was transferred to a centrifuge tube, and after high-speed centrifugation, the supernatant and the lower layer of chitosan were obtained. -IR) spectrometer to analyze the total reflection infrared light ATR-FTIR spectrum of chitosan before adsorption an...

Embodiment 5

[0069] Measure 100mL of cadmium ion adsorption solution conical flasks with a concentration of 20ug / mL, adjust the pH value of the cadmium ion adsorption solution in each conical flask with hydrochloric acid solution and sodium hydroxide solution, and adjust the pH value to 7.0 , add 6,000,000 strains of Bacillus subtilis, seal the mouth of the Erlenmeyer flask with plastic wrap, and then place the Erlenmeyer flask in a constant temperature oscillator to vibrate at a vibration speed of 120r / min and an oscillation temperature of 25°C; stop shaking after 8h , the mixture in the Erlenmeyer flask was transferred to a centrifuge tube, and after high-speed centrifugation, the supernatant and the lower layer of Bacillus subtilis were obtained, and the microstructure of the cell surface before and after the Bacillus subtilis daughter-in-law was observed with a scanning electron microscope. The results are shown in Figure 5 .

[0070] Figure 5 Middle (a) is the SEM image of Bacillus...

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Abstract

The invention discloses a method using the synergetic effect of chitosan and bacillus subtilis to remove heavy metals in water. The method comprises the following steps: (1) preparing a lead-cadmium-mercury mixed adsorption solution, a lead ion adsorption solution, a cadmium ion adsorption solution, and a mercury ion adsorption solution; (2) respectively taking chitosan, bacillus subtilis, and a mixture of chitosan and bacillus subtilis as the heavy metal absorbent to adsorb the adsorption solutions in the step (1), and comparing the adsorbing effect of three adsorbents. The invention researches the synergetic adsorption effect of chitosan and bacillus subtilis on heavy metals (lead, cadmium, and mercury). When chitosan and bacillus subtilis are used together, chitosan can form a thick and loosened layer on the surface of bacillus subtilis, metal ions can freely enter the layer and carry out chelation reactions; due to the large surface area of bacteria, the chitosan can fully spread, more binding sites are exposed, and thus the heavy metal adsorption performance is largely improved.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a research method for removing heavy metals in water by utilizing the synergistic effect of chitosan and bacillus subtilis. Background technique [0002] In the field of environment and human health, heavy metals mainly refer to mercury (Hg), cadmium (Cd), lead (Pb), chromium (Cr), arsenic (As), copper (Cu), zinc (Zn), cobalt (Co), Heavy metals such as nickel (Ni). They exist in the environment in different forms, and migrate and accumulate in the environment. Heavy metals are mainly used in mining, metallurgy, chemical and other industries. Drainage in mine pits, tailings drainage in mineral processing plants, dust removal drainage in smelters, washing water in electroplating plants, electrolysis, pesticides and pigments and other industrial production waste water contain a large amount of heavy metals. Untreated or substandard wastewater containing heavy ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/20
CPCC02F1/286C02F3/34C02F2101/20
Inventor 石岩孙垦张堯
Owner NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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