The invention provides a rapid and high-flux rabbit polyclonal
antibody in-vitro production method,. According to the method, a B
lymphocyte separation technology is mainly adopted to realize rapid, large-scale and stable production of rabbit
polyclonal antibodies through in-vitro amplification culture, a B
lymphocyte culture medium is optimized,
tryptone is adopted, and through cooperation with blank rabbit serum, the B
lymphocyte culture medium is prepared. The differentiation and proliferation amount of B lymphocytes are greatly improved, and a basis of
cell quantity is provided for in-vitro production of the rabbit
polyclonal antibodies. Through the method provided by the invention, the rabbits can be immediately subjected to
blood sampling for production after being immunized for thethird time, the
production cycle of products is greatly shortened, and the yield of the obtained rabbit
polyclonal antibodies is 30 times or above of that of serum with the same volume in the traditional method. The method has the advantages of
short cycle, high yield, low
unit cost, stable quality and the like, and is suitable for large-scale production and application.