Source component and device for inducing high-voltage impulse wave to separate single cells through laser photolysis

A source and receiver technology, applied in the field of single cell separation, can solve the problems of multiple cells in the droplet and uneven distribution of cells

Active Publication Date: 2017-03-22
GUANGDONG UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0004] In view of this, the present invention provides a source and a laser photolysis-induced high-voltage pulse wave separation device for single cells, which is used to solve the technical problems of uneven distribution of cells in cell culture fluid and a single sputtered droplet containing multiple cells

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  • Source component and device for inducing high-voltage impulse wave to separate single cells through laser photolysis
  • Source component and device for inducing high-voltage impulse wave to separate single cells through laser photolysis
  • Source component and device for inducing high-voltage impulse wave to separate single cells through laser photolysis

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Embodiment Construction

[0026] The technical solution of the present invention will be clearly and completely described in conjunction with the accompanying drawings of the specific description of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all of them. Those skilled in the art should understand that any modification to the specific embodiments of the present invention or equivalent replacement of some technical features without departing from the spirit of the technical solution of the present invention shall be covered by the protection scope of the present invention.

[0027] Please refer to figure 1 and figure 2 A source element provided by the present invention includes sequentially arranged: a glass slide 1, a sacrificial layer 2 and a microarray hole structure 3, and the glass slide 1, the sacrificial layer 2 and the microarray hole structure 3 are bonded and connected.

[0028] Wherein, the glass slide 1 is a quartz gla...

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Abstract

The invention belongs to the technical field of single cell separation and specifically relates to a source component and a device for inducing a high-voltage impulse wave to separate single cells through laser photolysis. In the source component provided by the invention, the original cell culture layer is designed as a micro-array pore structure and a cell culture fluid fills the through holes on the micro-array pore structure, so that the cells in the culture fluid are firstly pre-separated, the situation of one sputtering liquid drop containing a plurality of cells is avoided and the cell transferring precision and the separation efficiency are increased. According to the invention, the source component and a packaging component are assembled into a packaged structure, so that a sealed technological environment is formed in a single cell separation area, the suitable air humidity is guaranteed, the cell inactivation caused by dry air is prevented and the excellent cell survival environment is maintained.

Description

technical field [0001] The invention belongs to the technical field of single cell separation, and in particular relates to a source element and a device for separating single cells induced by high-voltage pulse waves induced by laser photolysis. Background technique [0002] Single cell technology takes single cells as the research direction to study the growth, metabolism and apoptosis of single cells. Recently, single cells have been widely used in biomedical fields such as personalized therapy, tumors, cardiovascular and cerebrovascular diseases, fertility and AIDS. Single cell isolation technology can provide research samples for single cell research, which is a crucial step in the realization of single cell technology. [0003] At present, there are many methods for separating single cells. In the previous research of the present invention, laser photolysis was used to induce high-voltage pulses to separate single cells. The main principle is: use a laser to generate ...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/22
CPCC12M23/10C12M23/12C12M35/02
Inventor 邓宇王文兵郭钟宁张永康黄志刚刘江文朱紫红麦文豪洪文生
Owner GUANGDONG UNIV OF TECH
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