121 results about "Bisulfite salt" patented technology

Method for characterizing, classifying and differentiating tissues and cell types, for predicting the behavior of tissues and groups of cells, and for identifying genes with changed expression. The method involves obtaining genomic DNA from a tissue sample, the genomic DNA subsequently being subjected to shearing, cleaved by means of a restriction endonuclease or not treated by either one of these methods. The base cytosine, but not 5-methylcytosine, from the thus-obtained genomic DNA is then converted into uracil by treatment with a bisulfite solution. Fractions of the thus-treated genomic DNA are then amplified using either very short or degenerated oligonucleotides or oligonuclcotides which are complementary to adaptor oligonucleotides that have been ligated to the ends of the cleaved DNA. The quantity of the remaining cytosines on the guanine-rich DNA strand and / or the quantity of guanines on the cytosine-rich DNA strand from the amplified fractions are then detected by hybridization or polymerase reaction, which quantities are such that the data generated thereby and automatically applied to a processing algorithm allow the drawing of conclusions concerning the phenotype of the sample material.

Methods for determining the methylation status of a plurality of cytosines are disclosed. In some aspects genomic DNA target sequences containing CpGs are targeted for analysis by multiplex amplification using target specific probes that can be specifically degraded prior to amplification. The targets may be modified with bisulfite prior to amplification. In another aspect targets are cut with methylation sensitive or insensitive restriction enzymes and marked with a tag using the target specific probes. The presence or absence of methylation may be determined using methylation sensitive restriction enzyme or bisulfite treatment. Detection in many embodiments employs hybridization to tag arrays, genotyping arrays or resequencing arrays.

Antiviral protease inhibitors, including peptidyl aldehydes, peptidyl α-ketoamides, peptidyl bisulfite salts, and peptidyl heterocycles, are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

Disclosed is a method for controlling microbial growth in an aqueous system containing sulfite and / or bisulfite residues by addition of a peroxy compound at a pH of greater than 5. Also disclosed is a method for stabilizing an active halogen biocide in an aqueous system containing peroxide residues by addition of an N-hydrogen compound to the active halogen biocide before combining it with the peroxide containing aqueous system. Further disclosed is an optimized papermaking biocide program consisting of initially treating sulfite bleached pulp with peroxide followed by application of an N-hydrogen-stabilized active halogen compound to the paper producing white waters and an analytical method for determining peroxide concentrations in aqueous systems in the presence of sulfite and / or bisulfite.

Described herein are methods, compositions and kits for the generation of bisulfite-converted libraries useful for conducting reduced representation bisulfite sequencing (RRBS). The methods described herein can be employed to generate RRBS libraries in a manner that is easier and more cost-efficient than conventional RRBS methods, and can be efficiently sequenced with next generation sequencing (NGS) techniques without the need for genomic, higher diversity sequencing controls such as PhiX spike-ins.

The present invention provides methods for improving the efficiency of methylation detection. The present invention provides improvements from current technologies via increased efficiency of template denaturation and bisulfite conversion reaction, also significant time savings in sample preparation, recovery, as well as increased efficiency of desulfonation. These methods facilitate rapid analysis of research and clinical samples and enhance the ability to process high-through put sample preparations. The methods are applicable to essentially all methylation detection procedures and also to the analysis of methylation patterns from various species.

The invention relates to the technical field of bioinformatics, particularly to a whole-genome methylation non-bisulfite sequencing library, a construction and applications thereof, wherein the sequencing library comprises a TET enzyme reaction solution, the TET enzyme reaction solution comprises the following independently packaged components: a TET enzyme oxidation buffer solution, and the TET enzyme oxidation buffer solution comprises, by micromole, (20-167)*10<3> parts of HEPES or Tris-Cl, (100-333)*10<3> parts of NaCl, 3.3*10<3> parts of alpha-KG or 2-oxoglutarate, 6.67*10<3> parts of ascorbic acid and 4*10<3> parts of adenosine triphosphate. According to the invention, by combining the kit, Fe(NH4)2(SO4)2 and TET enzyme, 5mc can be oxidized into 5cac, the 5cac is reduced into dihydrouracil under the action of a reducing agent, and T is identified through PCR sequencing, so that the the DNA methylation C-to-T conversion under the non-bisulfite condition is achieved, the defects ofbase imbalance and low sequencing data use rate of the existing methylation sequencing library constructed based on the bisulfite conversion are solved; and the formula further has effects of simplecomponents, extremely low TET enzyme use amount and significant cost reducing.

The process of invention provides a simplified conversion of sulfur dioxide gas into hydrogen sulfide gas. First, sulfur dioxide gas is absorbed into an aqueous sulfide solution to form sulfite and bisulfide ions. Second, additional sulfur dioxide gas is absorbed into the aqueous solution to form hydrogen sulfide. Third, another portion of the sulfur dioxide is absorbed and reacts in the aqueous solution to form bisulfite. Most advantageously, the bisulfite is decomposed into sulfur dioxide and sulfite. The sulfite is then reduced to sulfide and returned for use in the absorption process. The hydrogen sulfide may then be reacted with SO2 via the Claus reaction to form elemental sulfur.

The present invention provides a high-throughput sequencing method for methylated DNA, and use thereof. Particularly, the present invention provides a high-throughput sequencing method for methylated DNA, which combines methylated DNA immunoprecipitation, removal of repetitive sequences, and bisulfite treatment. The site of sequencing library will be decreased, and the cost will be reduced by using the method disclosed in the present invention.

The invention discloses a method and a system for preparing high-purity taurine and salt. The method comprises the following steps: reacting ethylene oxide with bisulfite to generate hydroxyethyl sulfonate; carrying out ammonolysis reaction on the hydroxyethyl sulfonate, ammonia and metal salt, evaporating to obtain a taurine salt concentrate, carrying out ion exchange on the concentrate in an ionexchange system to obtain an adsorption solution with taurine as a main component, independently collecting the adsorption solution, and extracting taurine from the adsorption solution; and eluting the adsorbed metal cations by using acid, independently collecting eluate, and extracting salt from the eluate or directly taking the eluate as a salt solution product. According to the method, materials of two target products are separated in advance after ammonolysis reaction, and then the target products are extracted respectively, so that the extraction process is very simple, interference on product separation when the two target products are in the same maternal system does not need to be considered at all, the process is simple, and the production cost is greatly reduced.

Methods of amplifying nucleic acid are described. Primers on solid support, e.g. a population of beads, are employed. A population of nucleic acid template molecules, wherein the nucleic acid template molecules have been treated with bisulfite, is amplified so as to create loaded beads comprising amplified nucleic acid.

The invention provides a method for simultaneously detecting a methylation level, genome variation and an insert fragment by utilizing a methylation non-bisulfite sequencing technology. The genome variation comprises gene mutation, copy number variation and structural variation. The invention also provides a device and equipment for implementing the method, and a corresponding computer readable medium. According to the invention, for methylated non-bisulfite sequencing data, one-step detection and analysis from offline data to multiple dimensions of methylation level, genome variation and insert fragments are realized, the method is suitable for whole genome and targeted capture data types of methylated non-bisulfite, and analysis of a single cancer sample and paired samples (cancer samples containing control samples) can be carried out.

Described herein are methods of preparing dual-indexed nucleic acid libraries for methylation profiling using bisulfite conversion sequencing. In various embodiments, the methods use a two-step indexing process to tag bisulfite-treated DNA with unique molecular identifiers (UMIs).

Provided are methods for the pretreatment of ligno-cellulosic biomass such as softwoods with bisulfite such as ammonium bisulfite without the need for exogenous acid. In one variation, a method of pretreating ligno-cellulosic biomass is provided including the following steps: a) providing ligno-cellulosic biomass; b) contacting the ligno-cellulosic biomass with a solution comprising bisulfite at an amount between 1 and 10% of a dry weight of the ligno-cellulosic biomass to form a slurry; c) heating the slurry to a first temperature of 150-210° C. for a first period of time to form a first mixture; d) cooling the first mixture to a second temperature of 100-200° C. to form a second mixture; and e) maintaining the second mixture at the second temperature for a second period of time to form pretreated ligno-cellulosic biomass; wherein the first temperature is higher than the second temperature.

The present invention provides acylalkylisethionate esters useful in consumer products. The acylalkylisethionate esters are produced by reacting one or more carboxylic acids with one or more alkyl-substituted hydroxyalkyl sulfonates under esterification reaction conditions. The alkyl-substituted hydroxyalkyl sulfonates used as a raw material in producing the esters are prepared by reacting bisulfite with one or more alkylene oxides.

The invention relates to a kit for the identification of gastric cancer and / or gastric polyps and application thereof. The kit comprises a primer pair, wherein the primer pair is used for detecting the methylation level of a biological marker gene or corresponding segments in a biological sample of a tester; the primer pair is used for performing PCR (polymerase chain reaction) amplification reaction by using the biological marker gene or segments thereof treated by bisulfite as a template; the biological marker gene is selected from one or multiple of CDH1, DAPK, PAX5, RASSF1A, Reprimo, RNF180, RUNX3, SDC2, Septin9 and TCF4. The kit has the advantage that by jointly detecting the methylation level of the biological marker gene or corresponding segments, the sensitivity and specificity indetection of the gastric cancer and / or gastric polyps is improved, so that the correctness and reliability of the detection results are guaranteed, and a quick, reliable and accurate novel path is provided for the predicting, diagnosis and evaluation on the gastric cancer and / or gastric polyps.

A corrosion control process is described. The process is especially useful in the control of chloride corrosion in waste to energy boilers. Corrosion of high temperature surfaces can be assessed by the monitor and controlled introduction of treatment chemicals by targeted in furnace injection reduces corrosion while maximizing combustion efficiency. A corrosion monitor is also described. Before and following selection of corrosion control chemicals and the locations for targeted in furnace injection, injection regimen and chemical selection and introduction parameters are monitored with the aid of the method and apparatus of the invention to adjust one or more control parameters to reduce corrosion. A preferred method will employ a treatment chemical that comprises an SO2 or SO3 reagent, e.g., sulfuric acid, sulfur, a sulfate salt or a bisulfite salt.

Methods of determining methylation of DNA are provided. In one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising a nucleic acid to a first matrix material comprising a first column or filter where said matrix material binds and / or filters nucleic acids in said sample and thereby purifies the DNA; ii) eluting the bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bi sulfite ions to produce a deaminated nucleic acid; iv) contacting said deaminated nucleic acid to a second matrix material comprising a second column to bind said deaminated nucleic acid to said second matrix material; v) desulphonating the bound deaminated nucleic acid and / or simultaneously eluting and desulphonating the nucleic acid by contacting the deaminated nucleic acid with an alkaline solution to produce a bi sulfite converted nucleic acid; vi) eluting said bi sulfite converted nucleic acid from said second matrix material; and vii) performing methylation specific PCR and / or nucleic acid sequencing, and / or high resolution melting analysis (HRM) on said bisulfite-converted nucleic acid to determine the methylation of said nucleic acid, wherein at least steps iv) through vi) are performed in a single reaction cartridge.

The invention relates to a method of preparing bisulfite-treated DNA library by ligation of adaptors to DNA after bisulfite conversion. The prepared library is suitable for use in sequencing reactions to analyze genome-wide DNA methylation status.

NO is removed from a gas stream by reacting NO with an absorbent to form a metal nitrosyl complex. The metal nitrosyl complex is reacted with sulfite and / or bisulfite to produce recoverable reaction products containing nitrogen and / or sulfur and to regenerate the reagent. The recoverable reaction products are then separated from the regenerated reagent.

An iron control agent capable of reducing ferric iron containing compounds to ferrous iron containing compounds in an acidic solution, such as one used for formation acidizing. The iron control agent comprises a combination of a sulfur dioxide, sulfurous acid, sulfite salts, bisulfite salts, or thiosulfate salts or mixtures thereof, with a source of copper ions and a source of iodine or iodine ions. The iron control agent may also include small amounts of an adjunct such as stannous chloride, 2-mercaptoethanol, and thioglycolic acid and its salts.

The present invention provides a high-throughput sequencing method for methylated DNA, and use thereof. Particularly, the present invention provides a high-throughput sequencing method for methylated DNA, which combines methylated DNA immunoprecipitation, removal of repetitive sequences, and bisulfite treatment. The site of sequencing library will be decreased, and the cost will be reduced by using the method disclosed in the present invention.

The invention provides a method for simultaneously detecting genomic DNA polymorphism and methylation. The method comprises the following steps: performing DNA fragmentation, and digesting the 3' endof the DNA fragment with DNA exonuclease; adding a dNTPs mixture containing 5-hydroxymethylcytosine deoxyribonucleotide, and performing completion the 3' end of the DNA fragment; performing bisulfiteconversion treatment on the modified DNA fragment and connecting an adaptor adapted to a high-throughput sequencing platform, and finally constructing a sequencing library by PCR amplification. By combining a double-end sequencing mode of the high-throughput sequencing platform, genomic DNA polymorphism and methylation detection can be performed simultaneously, and the goal of simultaneous detection of genome and epigenome is achieved.

The invention is to improved compositions and methods for the detection of target bisulfite converted methylated nucleotide sequences.

The invention discloses a kit for bisulfite conversion of free DNA. The kit comprises a bisulfite solution, a protective solution, washing liquor A, a magnetic bead suspension, washing liquor B, washing liquor C and eluate, wherein the protective solution is prepared from the following ingredients: 80% to 85% of tetrahydrofuran furfuryl alcohol, 10% to 15% of D-alpha tocopherol cetomacrogol 1000 succinate, 0.2% to 0.3% of tetrahydroglycosyl acetate, 0.05% to 0.1% of ethoxychrysoidine and the balance of water. The invention further provides a method for bisulfite conversion and DNA purificationof the free DNA by using the kit. According to the kit, a sulfite conversion method is subjected to great technological improvement, so that ctDNA conversion can be completed rapidly (40 to 60 minutes) under mild conditions (70 DEG C to 80 DEG C), pH of a system can be stabilized, ctDNA degradation is prevented, high-conversion-ratio, high-quality and high-purity free DNA can be rapidly obtained,and full automated operation of methylation researches is facilitated.

The invention relates to a polyurethane elastic cross-linking agent, a high-strength and high-toughness vegetable protein adhesive and application. The polyurethane elastic cross-linking agent is prepared from the following raw materials: isocyanate, polyol, a catalyst, 2,2-dimethylolpropionic acid, a small molecular diol chain extender, plant polyphenol, a neutralizer and bisulfite. The polyurethane elastic cross-linking agent provided by the embodiment of the invention has excellent compatibility. The vegetable protein adhesive prepared from the polyurethane elastic cross-linking agent is synchronously enhanced in the bonding strength and toughness. According to the invention, the polyurethane elastomer is used as an energy dissipation element, and a physical-covalent double cross-linkednetwork is constructed in a protein matrix through main chain design, so that the design of the high-strength high-toughness vegetable protein adhesive is realized.