The invention provides a test strip and a method for detecting
prostate tumor antigens. By the aid of the test strip and the method, the shortcomings of complicated operation and long
time consuming of existing methods for detecting PSA (
prostate specific antigens) and difficulty in meeting POC (
point of care) quick diagnosis requirements can be overcome. PSA
monoclonal antibodies II are labeled by gold nanorods by the aid of immunochromatography technologies, a
nitrocellulose membrane is coated with goat anti-rat IgG and PSA
monoclonal antibodies I, and the test strip for detecting the PSA isprepared by the aid of double-
antibody sandwich processes. Liquid can flows through a binding pad and the
nitrocellulose membrane step by step under capillary actions after PSA sample solution is added onto a sample pad of the test strip, and is bound with biological molecules, which are immobilized on the test strip, under
antigen-
antibody interaction, and a detection line visible to naked eyescan be generated. The test strip and the method are combined with
microarray scanners, so that corresponding detection
signal strength values can be obtained, and the PSA can be detected. The test strip and the method have the advantages that the method is simple and speedy, and is low in sample consumption and cost and short in detection time, the minimum concentration of the detectable PSA is 0.1 ng mL<-1>, the detection range is 0.1-100 ng mL<-1>, and clinical detection requirements can be met.