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Test strip and method for detecting prostate tumor antigens

A prostate tumor and test strip technology, which is applied in the directions of measuring devices, instruments, disease diagnosis, etc., can solve the problems of difficulty in meeting the rapid diagnosis of POC, time-consuming, complicated operation, etc., and achieves short detection time, meets clinical detection, and is simple in method. fast effect

Inactive Publication Date: 2018-08-07
上海格荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a test strip and method for detecting prostate tumor antigen, which can solve the shortcomings of the existing method for detecting PSA, which is complicated to operate, takes a long time, and is difficult to meet the rapid diagnosis of POC

Method used

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  • Test strip and method for detecting prostate tumor antigens
  • Test strip and method for detecting prostate tumor antigens
  • Test strip and method for detecting prostate tumor antigens

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preparation example Construction

[0035]The preparation method of described primary test strip comprises the following steps:

[0036] After pasting the nitrocellulose membrane on the corresponding area of ​​the backboard, mix goat anti-mouse IgG and PSA monoclonal antibody I with the antibody diluent respectively, and draw lines on the nitrocellulose membrane with a three-dimensional film-drawing device. The volume is set to 1 μL cm -1 , and then dried overnight at 37°C;

[0037] Soak the sample pad with the treatment solution in advance and dry it in vacuum at 37°C, then attach the treated sample pad, bonding pad, and water-absorbing pad to the corresponding areas of the backplane, and use an automatic cutting machine to cut according to the predetermined width to obtain a tape Primary test strips with control and test lines.

[0038] The preparation of the gold nanorod conjugates comprises the following steps:

[0039] After diluting the gold nanorod conjugate modified by PSA monoclonal antibody II with ...

Embodiment 1

[0050] After affixing the nitrocellulose membrane to the corresponding area of ​​the back plate, mix the goat anti-mouse IgG and PSA monoclonal antibody I with the antibody diluent at a concentration of 1 mg mL -1 , the antibody diluent consists of 3% sucrose and 0.2mg mL -1 NaN 3 0.01mol L -1 Phosphate buffer solution (pH=7.4). The HM3030 three-dimensional film scriber was used to draw a line on the nitrocellulose membrane, and the amount of the line was set to 1 μL cm -1 , and dried overnight at 37°C. Pre-soak the sample pad with the treatment solution (20mM Tris-HCl, pH=8.0, 0.1% Tween 20, 0.5% PVP) and dry it in vacuum at 37°C for 2 hours, then paste the treated sample pad, the binding pad and the absorbent pad respectively In the corresponding area of ​​the back plate, use the ZQ2002 automatic cutting machine to cut according to the width of 3mm, and obtain the test strip with the control line and the detection line.

[0051] After diluting the gold nanorod conjugate...

example 2

[0061] After affixing the nitrocellulose membrane to the corresponding area of ​​the back plate, mix the goat anti-mouse IgG and PSA monoclonal antibody I with the antibody diluent at a concentration of 1 mg mL -1 , the antibody diluent consists of 3% sucrose and 0.2mg mL -1 NaN 3 0.01mol L -1 Phosphate buffer solution (pH=7.4). The HM3030 three-dimensional film scriber was used to draw a line on the nitrocellulose membrane, and the amount of the line was set to 1 μL cm -1, and dried overnight at 37°C. Pre-soak the sample pad with the treatment solution (20mM Tris-HCl, pH=8.0, 0.1% Tween 20, 0.5% PVP) and dry it in vacuum at 37°C for 2 hours, then paste the treated sample pad, the binding pad and the absorbent pad respectively In the corresponding area of ​​the back plate, use the ZQ2002 automatic cutting machine to cut according to the width of 3mm, and obtain the test strip with the control line and the detection line.

[0062] After diluting the gold nanorod conjugate ...

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Abstract

The invention provides a test strip and a method for detecting prostate tumor antigens. By the aid of the test strip and the method, the shortcomings of complicated operation and long time consuming of existing methods for detecting PSA (prostate specific antigens) and difficulty in meeting POC (point of care) quick diagnosis requirements can be overcome. PSA monoclonal antibodies II are labeled by gold nanorods by the aid of immunochromatography technologies, a nitrocellulose membrane is coated with goat anti-rat IgG and PSA monoclonal antibodies I, and the test strip for detecting the PSA isprepared by the aid of double-antibody sandwich processes. Liquid can flows through a binding pad and the nitrocellulose membrane step by step under capillary actions after PSA sample solution is added onto a sample pad of the test strip, and is bound with biological molecules, which are immobilized on the test strip, under antigen-antibody interaction, and a detection line visible to naked eyescan be generated. The test strip and the method are combined with microarray scanners, so that corresponding detection signal strength values can be obtained, and the PSA can be detected. The test strip and the method have the advantages that the method is simple and speedy, and is low in sample consumption and cost and short in detection time, the minimum concentration of the detectable PSA is 0.1 ng mL<-1>, the detection range is 0.1-100 ng mL<-1>, and clinical detection requirements can be met.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a test strip and a method for detecting prostate tumor antigens. Background technique [0002] During the process of normal cells becoming cancerous, the content of carbohydrate antigens on the surface of tumor cells will increase. After these antigens fall off, they will enter the blood and become tumor markers in serum. Prostate cancer is the most common malignant tumor of the male genitourinary system, accounting for the second highest male mortality rate of malignant tumors. The most commonly used examination is prostate-specific antigen (PSA) screening, which is to measure the level of PSA in serum. As a marker of prostate cancer, PSA has the characteristics of strong sensitivity and specificity and non-invasive examination, and plays an important role in the diagnosis of prostate cancer. PSA is a tissue-specific glycoprotein synthesized mainly by prostatic alveolar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574
CPCG01N33/57434G01N33/577G01N2800/342
Inventor 王振新张婳
Owner 上海格荣生物科技有限公司
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