Method of detecting biopolymer, biochip, method of fixing antibody, and substrate for fixing antibody thereon

Abstract

The present invention relates to a biopolymer detection method based on antigen-antibody interaction, wherein the method shows improved S / N ratio, improved detection sensitivity and reduced detection time. The present invention also relates to the application of these methods on biochips. The present invention also relates to an antibody immobilization method in which antibody molecules are immobilized on an amide group-containing gel, or an amide group-containing gel on an insoluble substance. Use of such gels prevents non-specific adsorption of antibody binding molecules. By embedding these antibody-binding molecules in such a gel, degradation of their antibody-binding activity is prevented. A method for detecting a biopolymer by capturing a target biopolymer to a substrate surface, comprising the following steps: (1) attaching a target biopolymer, together with a probe biopolymer and an antibody or address probe peptide or a peptide bound to them (2) hybridize the target biopolymer to the probe biopolymer; and (3) through the antigen-antibody interaction, through the address probe peptide Or biopolymer or polyclonal antibody molecules to recognize the address linker bound to the substrate. The method of immobilizing antibodies comprises the steps of: (1) applying a gel containing amide groups embedded with antibody binding molecules on a matrix of insoluble substances in two or three dimensions; and (2) attaching the bases of antibody molecules to the antibody binding molecule.

Description

technical field [0001] The present invention describes methods for detecting biopolymers such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and proteins; and biochips using these methods. Furthermore, the present invention relates to methods for immobilizing antibodies, and substrates on which antibodies are immobilized. Background technique [0002] A technique for detecting biopolymers (DNA is exemplified hereinafter) by using a microarray is known, for example, as described in Unexamined Published Japanese Patent Application No. (JP-A) 2000-131237 that kind of technology. These types of DNA microarrays are typically formed and used to detect DNA as follows: [0003] Arrays of DNA probes containing sequences complementary to the target mRNA (or cDNA) are spotted and then immobilized on a glass (or plastic) substrate. This matrix is ​​contacted with a set of fluorescently labeled target mRNAs (or cDNAs) in solution. At this point, the complementary probe hybrid...

AI Technical Summary

Problems solved by technology

Each step in each protocol creates many problems, including data accuracy, reproducibility, repeatability, and sensitivity, which prevents the standardization of experimental data

In addition to the difficulty in target cDNA selection, that is, the difficulty of disease contents sequence selection, these problems have hindered the successful clinical application of DNA microarray technology.

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